Abstract
A commercially available agar-slide blood culture bottle (Septi-Chek; Roche Diagnostics, Div. Hoffman-La Roche, Inc., Nutley, N.J.) was compared with the radiometric blood culture system (BACTEC; Johnston Laboratories, Inc., Towson, Md.) in 8,544 paired blood cultures from adult patients. The systems were inoculated with equal volumes (10 ml) of blood. Overall, there was no statistically significant difference between the two systems in the recovery of clinically important microorganisms, but significantly more members of the family Enterobacteriaceae other than Escherichia coli were detected by the agar-slide system (P less than 0.005). The agar-slide system detected more fungi, and the BACTEC detected more anaerobic bacteria; however, small numbers of recovered organisms precluded statistical significance. When microorganisms grew in both systems, their presence was detected one or more days earlier in the BACTEC (P less than 0.001). More contaminants grew in the agar-slide system (P less than 0.001). Both systems performed well, and either system should provide high yield and prompt detection of positive blood cultures in patients with bacteremia and fungemia if used in an optimal way as recommended by the respective manufacturers.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Auckenthaler R., Ilstrup D. M., Washington J. A., 2nd Comparison of recovery of organisms from blood cultures diluted 10% (volume/volume) and 20% (volume/volume). J Clin Microbiol. 1982 May;15(5):860–864. doi: 10.1128/jcm.15.5.860-864.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brooks K., Sodeman T. Rapid detection of bacteremia by a radiometric system. A clinical evaluation. Am J Clin Pathol. 1974 Jun;61(6):859–866. doi: 10.1093/ajcp/61.6.859. [DOI] [PubMed] [Google Scholar]
- Bryan L. E. Comparison of a slide blood culture system with a supplemented peptone broth culture method. J Clin Microbiol. 1981 Oct;14(4):389–392. doi: 10.1128/jcm.14.4.389-392.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henry N. K., Grewell C. M., McLimans C. A., Washington J. A., 2nd Comparison of the Roche Septi-Chek blood culture bottle with a brain heart infusion biphasic medium bottle and with a tryptic soy broth bottle. J Clin Microbiol. 1984 Mar;19(3):315–317. doi: 10.1128/jcm.19.3.315-317.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ilstrup D. M., Washington J. A., 2nd Effects of atmosphere of incubation on recovery of bacteria and yeasts from blood cultures in Tryptic soy broth. Diagn Microbiol Infect Dis. 1983 Sep;1(3):215–219. doi: 10.1016/0732-8893(83)90020-2. [DOI] [PubMed] [Google Scholar]
- Ilstrup D. M., Washington J. A., 2nd The importance of volume of blood cultured in the detection of bacteremia and fungemia. Diagn Microbiol Infect Dis. 1983 Jun;1(2):107–110. doi: 10.1016/0732-8893(83)90039-1. [DOI] [PubMed] [Google Scholar]
- Mirrett S., Reller L. B. Comparison of direct and standard antimicrobial disk susceptibility testing for bacteria isolated from blood. J Clin Microbiol. 1979 Oct;10(4):482–487. doi: 10.1128/jcm.10.4.482-487.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pfaller M. A., Sibley T. K., Westfall L. M., Hoppe-Bauer J. E., Keating M. A., Murray P. R. Clinical laboratory comparison of a slide blood culture system with a conventional broth system. J Clin Microbiol. 1982 Sep;16(3):525–530. doi: 10.1128/jcm.16.3.525-530.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reimer L. G., McDaniel J. D., Mirrett S., Reller L. B., Wang W. L. Controlled evaluation of supplemented peptone and Bactec blood culture broths for the detection of bacteremia and fungemia. J Clin Microbiol. 1985 Apr;21(4):531–534. doi: 10.1128/jcm.21.4.531-534.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reimer L. G., Reller L. B., Mirrett S., Wang W. L., Cox R. L. Controlled evaluation of hypertonic sucrose medium at a 1:5 ratio of blood to broth for detection of bacteremia and fungemia in supplemented peptone broth. J Clin Microbiol. 1983 Jun;17(6):1045–1049. doi: 10.1128/jcm.17.6.1045-1049.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Renner E. D., Gatheridge L. A., Washington J. A., 2nd Evaluation of radiometric system for detecting bacteremia. Appl Microbiol. 1973 Sep;26(3):368–372. doi: 10.1128/am.26.3.368-372.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tenney J. H., Reller L. B., Mirrett S., Wang W. L., Weinstein M. P. Controlled evaluation of the volume of blood cultured in detection of bacteremia and fungemia. J Clin Microbiol. 1982 Apr;15(4):558–561. doi: 10.1128/jcm.15.4.558-561.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tenney J. H., Reller L. B., Mirrett S., Weinstein M. P., Wang W. L. Controlled evaluation of the effect of atmosphere of incubation on detection of bacteremia and fungemia in supplemented peptone broth. J Clin Microbiol. 1982 Sep;16(3):437–442. doi: 10.1128/jcm.16.3.437-442.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thiemke W. A., Wicher K. Laboratory experience with a radiometric method for detecting bacteremia. J Clin Microbiol. 1975 Mar;1(3):302–308. doi: 10.1128/jcm.1.3.302-308.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weinstein M. P., Murphy J. R., Reller L. B., Lichtenstein K. A. The clinical significance of positive blood cultures: a comprehensive analysis of 500 episodes of bacteremia and fungemia in adults. II. Clinical observations, with special reference to factors influencing prognosis. Rev Infect Dis. 1983 Jan-Feb;5(1):54–70. doi: 10.1093/clinids/5.1.54. [DOI] [PubMed] [Google Scholar]
- Weinstein M. P., Reller L. B., Mirrett S., Wang W. L., Alcid D. V. Clinical comparison of an agar slide blood culture bottle with tryptic soy broth and a conventional blood culture bottle with supplemented peptone broth. J Clin Microbiol. 1985 May;21(5):815–818. doi: 10.1128/jcm.21.5.815-818.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weinstein M. P., Reller L. B., Mirrett S., Wang W. L., Alcid D. V. Controlled evaluation of Trypticase soy broth in agar slide and conventional blood culture systems. J Clin Microbiol. 1985 Apr;21(4):626–629. doi: 10.1128/jcm.21.4.626-629.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weinstein M. P., Reller L. B., Murphy J. R., Lichtenstein K. A. The clinical significance of positive blood cultures: a comprehensive analysis of 500 episodes of bacteremia and fungemia in adults. I. Laboratory and epidemiologic observations. Rev Infect Dis. 1983 Jan-Feb;5(1):35–53. doi: 10.1093/clinids/5.1.35. [DOI] [PubMed] [Google Scholar]