Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Feb;23(2):235–239. doi: 10.1128/jcm.23.2.235-239.1986

Bordetella bronchiseptica phase variation induced by crystal violet.

H Ishikawa, Y Isayama
PMCID: PMC268619  PMID: 3700613

Abstract

A method for effective induction of phase variation in Bordetella bronchiseptica by treatment with crystal violet (CV) is presented. When grown in CV-broth, phase I cells dissociated into three serial phases. Appearance of variant cells was observed simultaneously with the beginning of cell multiplication. The maximum effect of CV was obtained at a concentration of 8 micrograms/ml, when the proportion of variants in the population reached 100%. The main factors which affected phase variation were concentration of CV, culture age, and temperature of treatment. The phase variants obtained were phenotypically stable upon serial passages on Bordet-Gengou agar plates. By this treatment, no reversion of phase descendants to former phases was observed.

Full text

PDF
235

Images in this article

238Image
on p.238

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baskerville M., Baskerville A., Wood M. A study of chronic pneumonia in a guineapig colony with enzootic Bordetella bronchiseptica infection. Lab Anim. 1982 Jul;16(3):290–296. doi: 10.1258/002367782780891787. [DOI] [PubMed] [Google Scholar]
  2. Batey R. G., Smits A. F. The isolation of Bordetella bronchiseptic from an outbreak of canine pneumonia. Aust Vet J. 1976 Apr;52(4):184–186. doi: 10.1111/j.1751-0813.1976.tb09469.x. [DOI] [PubMed] [Google Scholar]
  3. Bemis D. A., Carmichael L. E., Appel M. J. Naturally occurring respiratory disease in a kennel caused by Bordetella bronchiseptica. Cornell Vet. 1977 Apr;67(2):282–293. [PubMed] [Google Scholar]
  4. Bemis D. A., Greisen H. A., Appel M. J. Bacteriological variation among Bordetella bronchiseptica isolates from dogs and other species. J Clin Microbiol. 1977 Apr;5(4):471–480. doi: 10.1128/jcm.5.4.471-480.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bouanchaud D. H., Scavizzi M. R., Chabbert Y. A. Elimination by ethidium bromide of antibiotic resistance in enterobacteria and staphylococci. J Gen Microbiol. 1968 Dec;54(3):417–425. doi: 10.1099/00221287-54-3-417. [DOI] [PubMed] [Google Scholar]
  6. Chang K. C., Zakhein R. M., Cho C. T., Montgomery J. C. Letter: Posttraumatic purulent meningitis due to Bordetella bronchiseptica. J Pediatr. 1975 Apr;86(4):639–640. doi: 10.1016/s0022-3476(75)80178-8. [DOI] [PubMed] [Google Scholar]
  7. DUNNE H. W., KRADEL D. C., DOTY R. B. Bordetella bronchiseptica (Brucella bronchiseptica) in pneumonia in young pigs. J Am Vet Med Assoc. 1961 Oct 15;139:897–899. [PubMed] [Google Scholar]
  8. Eliás B., Krüger M., Rátz F. Epizootiologische Untersuchungen der Rhinitis atrophicans des Schweines. II. Biologische Eigenschaften der von Schweinen isolierten Bordetella bronchiseptica-Stämme. Zentralbl Veterinarmed B. 1982 Sep;29(8):619–635. [PubMed] [Google Scholar]
  9. Filion R., Cloutier S., Vrancken E. R., Bernier G. Infection respiratoire du dindonneau causée par un microbe apparenté au Bordetella bronchiseptica. Can J Comp Med Vet Sci. 1967 May;31(5):129–134. [PMC free article] [PubMed] [Google Scholar]
  10. Fisk S. K., Soave O. A. Bordetella bronchiseptica in laboratory cats from central California. Lab Anim Sci. 1973 Feb;23(1):33–35. [PubMed] [Google Scholar]
  11. GALLAGHER G. L. ISOLATION OF BORDETELLA BRONCHISEPTICA FROM HORSES. Vet Rec. 1965 May 29;77:632–633. [PubMed] [Google Scholar]
  12. Ghosh H. K., Tranter J. Bordetella bronchicanis (bronchiseptica) infection in man: review and a case report. J Clin Pathol. 1979 Jun;32(6):546–548. doi: 10.1136/jcp.32.6.546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Graves I. L. Bordetella bronchiseptica isolated from a fatal case of bronchopnuemonia in an African green monkey. Lab Anim Care. 1968 Jun;18(3):405–406. [PubMed] [Google Scholar]
  14. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jenkins E. M., Anthony V., Vance R. T., Cleveland J., Gbadamosi S. G. Prevalence of Bordetella bronchiseptica infection in swine of southeastern Alabama. Am J Vet Res. 1977 Dec;38(12):2071–2074. [PubMed] [Google Scholar]
  17. Kohn D. F., Haines D. E. Bordetella bronchiseptica infection in the lesser bushbaby (Galago senegalensis). Lab Anim Sci. 1977 Apr;27(2):279–280. [PubMed] [Google Scholar]
  18. Mather E. C., Addison B., Owens D., Bierschwal C. J., Martin C. E. Bordetella bronchiseptica associated with infertility in a mare. J Am Vet Med Assoc. 1973 Jul 1;163(1):76–77. [PubMed] [Google Scholar]
  19. McKenzie R. A., Wood A. D., Blackall P. J. Pneumonia associated with Bordetella bronchiseptica in captive koalas. Aust Vet J. 1979 Sep;55(9):427–430. doi: 10.1111/j.1751-0813.1979.tb05597.x. [DOI] [PubMed] [Google Scholar]
  20. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Oldenburg J., Köhler B., Fuchs H. W., Horsch F. Vorkommen und Bedeutung von Bordetella bronchiseptica beim Kaninchen. Monatsh Veterinarmed. 1972 Oct 1;27(19):738–743. [PubMed] [Google Scholar]
  22. Peppler M. S., Schrumpf M. E. Phenotypic variation and modulation in Bordetella bronchiseptica. Infect Immun. 1984 Jun;44(3):681–687. doi: 10.1128/iai.44.3.681-687.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rimler R. B., Simmons D. G. Differentiation among bacteria isolated from turkeys with coryza (rhinotracheitis). Avian Dis. 1983 Apr-Jun;27(2):491–500. [PubMed] [Google Scholar]
  24. Rubin S. J., Rosenblum E. D. Effects of ethidium bromide on growth and on loss of the penicillinase plasmid of Staphylococcus aureus. J Bacteriol. 1971 Dec;108(3):1200–1204. doi: 10.1128/jb.108.3.1200-1204.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. SAKAZAKI R., NAMIOKA S., OSADA A., YAMADA C. A problem on the pathogenic role of Citrobacter of enteric bacteria. Jpn J Exp Med. 1960 Feb;30:13–21. [PubMed] [Google Scholar]
  26. Seibold H. R., Perrin E. A., Jr, Garner A. C. Pneumonia associated with Bordetella bronchiseptica in Callicebus species primates. Lab Anim Care. 1970 Jun;20(3):456–461. [PubMed] [Google Scholar]
  27. Shimizu T., Ishikawa H. Some characteristics of a urease-negative, temperature- sensitive strain of Bordetella bronchiseptica as a live, attenuated vaccine. Infect Immun. 1982 Apr;36(1):198–201. doi: 10.1128/iai.36.1.198-201.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Snyder S. B., Fisk S. K., Fox J. G., Soave O. A. Respiratory tract disease associated with Bordetella bronchiseptica infection in cats. J Am Vet Med Assoc. 1973 Aug 1;163(3):293–294. [PubMed] [Google Scholar]
  29. Sonstein S. A., Baldwin J. N. Loss of the penicillinase plasmid after treatment of Staphylococcus aureus with sodium dodecyl sulfate. J Bacteriol. 1972 Jan;109(1):262–265. doi: 10.1128/jb.109.1.262-265.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Switzer W. P., Maré C. J., Hubbard E. D. Incidence of Bordetella bronchiseptica in wildlife and man in iowa. Am J Vet Res. 1966 Jul;27(119):1134–1136. [PubMed] [Google Scholar]
  31. Tomoeda M., Inuzuka M., Kubo N., Nakamura S. Effective elimination of drug resistance and sex factors in Escherichia coli by sodium dodecyl sulfate. J Bacteriol. 1968 Mar;95(3):1078–1089. doi: 10.1128/jb.95.3.1078-1089.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. WATANABE T., FUKASAWA T. Episome-mediated transfer of drug resistance in Enterobacteriaceae. II. Elimination of resistance factors with acridine dyes. J Bacteriol. 1961 May;81:679–683. doi: 10.1128/jb.81.5.679-683.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wagner J. E., Owens D. R., Kusewitt D. F., Corley E. A. Otitis media of guinea pigs. Lab Anim Sci. 1976 Dec;26(6 Pt 1):902–907. [PubMed] [Google Scholar]
  34. Yoda H., Nakayama K., Yusa T., Sato S., Fukuda S. [Bacteriolgical survey on Bordetella bronchiseptica in various animal species (author's transl)]. Jikken Dobutsu. 1976 Jan;25(1):7–11. [PubMed] [Google Scholar]
  35. Yokomizo Y., Shimizu T. Adherence of Bordetella bronchiseptica to swine nasal epithelial cells and its possible role in virulence. Res Vet Sci. 1979 Jul;27(1):15–21. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES