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. 1986 Feb;23(2):333–338. doi: 10.1128/jcm.23.2.333-338.1986

Serovar determination of Chlamydia trachomatis isolates by using type-specific monoclonal antibodies.

W J Newhall 5th, P Terho, C E Wilde 3rd, B E Batteiger, R B Jones
PMCID: PMC268637  PMID: 2422202

Abstract

A panel of 15 monoclonal antibodies was prepared that could distinguish among the 15 serovars of Chlamydia trachomatis. Twelve of these antibodies were specific for a single serovar (A, B, C, D, E, F, G, H, I, K, L1, and L2) and three were specific for two serovars (B/Ba, C/J, and C/L3). Ten of the serovar-specific and two of the bispecific antibodies were shown by immunoblotting to recognize epitopes on the major outer membrane protein. These data provide evidence that such epitopes are closely correlated with and may be partly responsible for the antigenic variations detected by microimmunofluorescence that distinguish the currently recognized serovars. When used in a radioimmunoassay, these antibodies correctly identified the serovar of 17 strains that had been serotyped by the microimmunofluorescence test. In addition, we found that the chlamydial antigen derived from 1.0 cm2 of an infected HeLa cell monolayer was sufficient to allow serotyping with these antibodies. Thus, these monoclonal antibodies may provide a rapid and reliable alternative to mouse immunization and microimmunofluorescence for serotyping of clinical isolates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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