Abstract
Although the Gram stain is usually used to screen sputum specimens prior to culture, wet mount observation with Nomarski optics has been suggested as a useful alternative. We compared the two methods and found that more specimens were rejected by the Gram stain technique without eliminating any that yielded important clinical information.
Full text
PDF

Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Geckler R. W., Gremillion D. H., McAllister C. K., Ellenbogen C. Microscopic and bacteriological comparison of paired sputa and transtracheal aspirates. J Clin Microbiol. 1977 Oct;6(4):396–399. doi: 10.1128/jcm.6.4.396-399.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heineman H. S., Chawla J. K., Lopton W. M. Misinformation from sputum cultures without microscopic examination. J Clin Microbiol. 1977 Nov;6(5):518–527. doi: 10.1128/jcm.6.5.518-527.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heineman H. S., Radano R. R. Acceptability and cost savings of selective sputum microbiology in a community teaching hospital. J Clin Microbiol. 1979 Oct;10(4):567–573. doi: 10.1128/jcm.10.4.567-573.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murray P. R., Washington J. A. Microscopic and baceriologic analysis of expectorated sputum. Mayo Clin Proc. 1975 Jun;50(6):339–344. [PubMed] [Google Scholar]
- Van Scoy R. E. Bacterial sputum cultures. A clinician's viewpoint. Mayo Clin Proc. 1977 Jan;52(1):39–41. [PubMed] [Google Scholar]
- Welch D. F., Kelly M. T. Sputum screening by Nomarski interference contrast microscopy. J Clin Microbiol. 1979 Apr;9(4):520–524. doi: 10.1128/jcm.9.4.520-524.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wong L. K., Barry A. L., Horgan S. M. Comparison of six different criteria for judging the acceptability of sputum specimens. J Clin Microbiol. 1982 Oct;16(4):627–631. doi: 10.1128/jcm.16.4.627-631.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
