Abstract
We explored the feasibility of using the passive hemagglutination (PHA) test in combination with the hemagglutination inhibition (HI) test for the single-serum diagnosis of rubella. We found by sedimentation analysis of the serum that early 7S antibody produced within 1 month after the onset of the rash had high HI but much lower PHA titers, whereas the PHA titers of antibody produced later were slightly higher than the HI titers. (19S antibody in the early serum had some PHA activity.) This disparity between the HI and PHA activities of the early 7S antibody could be used for the routine diagnosis of rubella. A comparison of the HI titers of the test serum with the PHA titers of mercaptoethanol-treated serum constitutes a simple method for determining whether the serum sample was taken shortly or remotely after the infection.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cremer N. E., Hagens S. J., Cossen C. K. Specificity of the passive hemagglutination test for antibody to rubella virus and the passive hemagglutination response after vaccination. J Clin Microbiol. 1981 Jan;13(1):226–227. doi: 10.1128/jcm.13.1.226-227.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drucker J., Smallwood L., Lorenz D., Tabor E., Coursaget P., Maupas P., Barker L. F., Gerety R. J. Serologic response in human hepatitis A: detection of antibody by radioimmunoassay and immune adherence hemagglutination. J Med Virol. 1979;4(1):51–58. doi: 10.1002/jmv.1890040107. [DOI] [PubMed] [Google Scholar]
- EISEN H. N., SISKIND G. W. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. Biochemistry. 1964 Jul;3:996–1008. doi: 10.1021/bi00895a027. [DOI] [PubMed] [Google Scholar]
- Griffiths G. M., Berek C., Kaartinen M., Milstein C. Somatic mutation and the maturation of immune response to 2-phenyl oxazolone. Nature. 1984 Nov 15;312(5991):271–275. doi: 10.1038/312271a0. [DOI] [PubMed] [Google Scholar]
- Inouye S., Hasegawa A., Matsuno S., Katow S. Changes in antibody avidity after virus infections: detection by an immunosorbent assay in which a mild protein-denaturing agent is employed. J Clin Microbiol. 1984 Sep;20(3):525–529. doi: 10.1128/jcm.20.3.525-529.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Inouye S., Kono R., Takeuchi Y. Oligomeric immunoglobulin A antibody response to rubella virus infection. J Clin Microbiol. 1978 Jul;8(1):1–6. doi: 10.1128/jcm.8.1.1-6.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Inouye S., Matsuno S., Kono R. Difference in antibody reactivity between complement fixation and immune adherence hemagglutination tests with virus antigens. J Clin Microbiol. 1981 Sep;14(3):241–246. doi: 10.1128/jcm.14.3.241-246.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Inouye S. Micro-modification of kaolin treatment of serum for the rubella haemagglutination-inhibition test. J Med Microbiol. 1976 Nov;9(4):501–502. doi: 10.1099/00222615-9-4-501. [DOI] [PubMed] [Google Scholar]
- Meurman O. H. Antibody responses in patients with rubella infection determined by passive hemagglutination, hemagglutination inhibition, complement fixation, and solid-phase radioimmunoassay tests. Infect Immun. 1978 Feb;19(2):369–372. doi: 10.1128/iai.19.2.369-372.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rakela J., Stevenson D., Edwards V. M., Gordon I., Mosley J. W. Antibodies to hepatitis A virus: patterns by two procedures. J Clin Microbiol. 1977 Jan;5(1):110–111. doi: 10.1128/jcm.5.1.110-111.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Siskind G. W., Benacerraf B. Cell selection by antigen in the immune response. Adv Immunol. 1969;10:1–50. doi: 10.1016/s0065-2776(08)60414-9. [DOI] [PubMed] [Google Scholar]
- Vesikari T., Vaheri A. Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies. Br Med J. 1968 Jan 27;1(5586):221–223. doi: 10.1136/bmj.1.5586.221. [DOI] [PMC free article] [PubMed] [Google Scholar]