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. 1986 Feb;23(2):395–397. doi: 10.1128/jcm.23.2.395-397.1986

Immunoblot analysis of the serological response in invasive Trichosporon beigelii and Blastoschizomyces capitatus infections.

R C Matthews, J P Burnie, A Fox, M Woods, S Tabaqchali
PMCID: PMC268655  PMID: 3517056

Abstract

The serological response to Trichosporon sp. was examined by the immunoblot technique. Antibodies to a range of antigens (200 to 16 kilodaltons) were detectable in three patients with invasive Trichosporon beigelii or Blastoschizomyces capitatus infections and 10 uninfected controls. High levels of preexisting antibodies may contribute to the rarity of systemic infections.

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Selected References

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  1. Arnold A. G., Gribbin B., De Leval M., Macartney F., Slack M. Trichosporon capitatum causing recurrent fungal endocarditis. Thorax. 1981 Jun;36(6):478–480. doi: 10.1136/thx.36.6.478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baird D. R., Harris M., Menon R., Stoddart R. W. Systemic infection with Trichosporon capitatum in two patients with acute leukaemia. Eur J Clin Microbiol. 1985 Feb;4(1):62–64. doi: 10.1007/BF02148664. [DOI] [PubMed] [Google Scholar]
  3. Brahn E., Leonard P. A. Trichosporon cutaneum endocarditis: a sequela of intravenous drug abuse. Am J Clin Pathol. 1982 Nov;78(5):792–794. doi: 10.1093/ajcp/78.5.792. [DOI] [PubMed] [Google Scholar]
  4. Burnie J. P., Matthews R. C., Fox A., Tabaqchali S. Use of immunoblotting to identify antigenic differences between the yeast and mycelial phases of Candida albicans. J Clin Pathol. 1985 Jun;38(6):701–706. doi: 10.1136/jcp.38.6.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans H. L., Kletzel M., Lawson R. D., Frankel L. S., Hopfer R. L. Systemic mycosis due to Trichosporon cutaneum: a report of two additional cases. Cancer. 1980 Jan 15;45(2):367–371. doi: 10.1002/1097-0142(19800115)45:2<367::aid-cncr2820450229>3.0.co;2-k. [DOI] [PubMed] [Google Scholar]
  6. Gold J. W., Poston W., Mertelsmann R., Lange M., Kiehn T., Edwards F., Bernard E., Christiansen K., Armstrong D. Systemic infection with Trichosporon cutaneum in a patient with acute leukemia: report of a case. Cancer. 1981 Nov 15;48(10):2163–2167. doi: 10.1002/1097-0142(19811115)48:10<2163::aid-cncr2820481008>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  7. Haupt H. M., Merz W. G., Beschorner W. E., Vaughan W. P., Saral R. Colonization and infection with Trichosporon species in the immunosuppressed host. J Infect Dis. 1983 Feb;147(2):199–203. doi: 10.1093/infdis/147.2.199. [DOI] [PubMed] [Google Scholar]
  8. Kiehn T. E., Edwards F. F., Armstrong D. The prevalence of yeasts in clinical specimens from cancer patients. Am J Clin Pathol. 1980 Apr;73(4):518–521. doi: 10.1093/ajcp/73.4.518. [DOI] [PubMed] [Google Scholar]
  9. Kirmani N., Tuazon C. U., Geelhoed G. W. Disseminated Trichosporon infection. Occurrence in an immunosuppressed patient with chronic active hepatitis. Arch Intern Med. 1980 Feb;140(2):277–278. doi: 10.1001/archinte.140.2.277. [DOI] [PubMed] [Google Scholar]
  10. Korinek J. K., Guarda L. A., Bolivar R., Stroehlein J. R. Trichosporon hepatitis. Gastroenterology. 1983 Sep;85(3):732–734. [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Matthews R. C., Burnie J. P., Tabaqchali S. Immunoblot analysis of the serological response in systemic candidosis. Lancet. 1984 Dec 22;2(8417-8418):1415–1418. doi: 10.1016/s0140-6736(84)91618-0. [DOI] [PubMed] [Google Scholar]
  14. McManus E. J., Jones J. M. Detection of a Trichosporon beigelii antigen cross-reactive with Cryptococcus neoformans capsular polysaccharide in serum from a patient with disseminated Trichosporon infection. J Clin Microbiol. 1985 May;21(5):681–685. doi: 10.1128/jcm.21.5.681-685.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'Connor C. G., Ashman L. K. Application of the nitrocellulose transfer technique and alkaline phosphatase conjugated anti-immunoglobulin for determination of the specificity of monoclonal antibodies to protein mixtures. J Immunol Methods. 1982 Oct 29;54(2):267–271. doi: 10.1016/0022-1759(82)90068-0. [DOI] [PubMed] [Google Scholar]
  16. Oelz O., Schaffner A., Frick P., Schaer G. Trichosporon capitatum: thrush-like oral infection, local invasion, fungaemia and metastatic abscess formation in a leukaemic patient. J Infect. 1983 Mar;6(2):183–185. doi: 10.1016/s0163-4453(83)92942-0. [DOI] [PubMed] [Google Scholar]
  17. Rivera R., Cangir A. Trichosporon sepsis and leukemia. Cancer. 1975 Sep;36(3):1106–1110. doi: 10.1002/1097-0142(197509)36:3<1106::aid-cncr2820360340>3.0.co;2-z. [DOI] [PubMed] [Google Scholar]
  18. Saul S. H., Khachatoorian T., Poorsattar A., Myerowitz R. L., Geyer S. J., Pasculle A. W., Ho M. Opportunistic Trichosporon pneumonia. Association with invasive aspergillosis. Arch Pathol Lab Med. 1981 Sep;105(9):456–459. [PubMed] [Google Scholar]
  19. Sheikh H. A., Mahgoub S., Badi K. Postoperative endophthalmitis due to Trichosporon cutaneum. Br J Ophthalmol. 1974 Jun;58(6):591–594. doi: 10.1136/bjo.58.6.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Terashima E., Tsuchiya T., Okuyama K. [Recent causal organisms of bacteremia and a case of septicemia caused by Trichosporon cutaneum (author's transl)]. Kansenshogaku Zasshi. 1978 Apr;52(4):102–104. doi: 10.11150/kansenshogakuzasshi1970.52.102. [DOI] [PubMed] [Google Scholar]
  21. Towbin H., Gordon J. Immunoblotting and dot immunobinding--current status and outlook. J Immunol Methods. 1984 Sep 4;72(2):313–340. doi: 10.1016/0022-1759(84)90001-2. [DOI] [PubMed] [Google Scholar]
  22. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Watson K. C., Kallichurum S. Brain abscess due to Trichosporon cutaneum. J Med Microbiol. 1970 Feb;3(1):191–193. doi: 10.1099/00222615-3-1-191. [DOI] [PubMed] [Google Scholar]
  24. Winston D. J., Balsley G. E., Rhodes J., Linné S. R. Disseminated Trichosporon capitatum infection in an immunosuppressed host. Arch Intern Med. 1977 Sep;137(9):1192–1195. [PubMed] [Google Scholar]
  25. Yung C. W., Hanauer S. B., Fretzin D., Rippon J. W., Shapiro C., Gonzalez M. Disseminated Trichosporon beigelii (cutaneum). Cancer. 1981 Nov 1;48(9):2107–2111. doi: 10.1002/1097-0142(19811101)48:9<2107::aid-cncr2820480932>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]

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