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. 1986 Mar;23(3):551–555. doi: 10.1128/jcm.23.3.551-555.1986

Clinical and epidemiological features of acute infantile gastroenteritis associated with human rotavirus subgroups 1 and 2.

I Uhnoo, L Svensson
PMCID: PMC268692  PMID: 3007567

Abstract

During a prospective 1-year study rotavirus isolates from 169 children with gastroenteritis were investigated by polyacrylamide gel electrophoresis. A total of 118 (70%) of the strains analyzed contained sufficient viral nucleic acid to give visible electrophoretic patterns; 36% were identified as strains belonging to subgroup 1 (short patterns), and 64% were identified as strains belonging to subgroup 2 (long patterns). The two subgroups cocirculated at equal frequencies during the first 7 months of the year, after which subgroup 1 rotavirus completely disappeared. Subgroup 2 rotavirus occurred throughout the year. No significant differences between the subgroups in relation to age or sex distribution were observed. Fever and temperatures exceeding 39 degrees C were significantly more frequent in children who shed rotavirus subgroup 1. Diarrhea and vomiting occurred at similar rates in both groups of patients, but were more pronounced in children who shed rotavirus subgroup 2. One of three dominant electropherotypic variants of subgroup 2 rotavirus was found to be associated with more intense symptoms, higher rates of hospitalization, and a significantly higher frequency of respiratory symptoms; the clinical picture may indicate that this rotavirus electropherotype has higher virulence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beards G. M., Pilfold J. N., Thouless M. E., Flewett T. H. Rotavirus serotypes by serum neutralisation. J Med Virol. 1980;5(3):231–237. doi: 10.1002/jmv.1890050307. [DOI] [PubMed] [Google Scholar]
  2. Beards G. M. Polymorphism of genomic RNAs within rotavirus serotypes and subgroups. Arch Virol. 1982;74(1):65–70. doi: 10.1007/BF01320783. [DOI] [PubMed] [Google Scholar]
  3. Brandt C. D., Kim H. W., Yolken R. H., Kapikian A. Z., Arrobio J. O., Rodriguez W. J., Wyatt R. G., Chanock R. M., Parrott R. H. Comparative epidemiology of two rotavirus serotypes and other viral agents associated with pediatric gastroenteritis. Am J Epidemiol. 1979 Sep;110(3):243–254. doi: 10.1093/oxfordjournals.aje.a112809. [DOI] [PubMed] [Google Scholar]
  4. Estes M. K., Palmer E. L., Obijeski J. F. Rotaviruses: a review. Curr Top Microbiol Immunol. 1983;105:123–184. doi: 10.1007/978-3-642-69159-1_3. [DOI] [PubMed] [Google Scholar]
  5. Follett E. A., Sanders R. C., Beards G. M., Hundley F., Desselberger U. Molecular epidemiology of human rotaviruses. Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83. J Hyg (Lond) 1984 Apr;92(2):209–222. doi: 10.1017/s0022172400064238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greenberg H., McAuliffe V., Valdesuso J., Wyatt R., Flores J., Kalica A., Hoshino Y., Singh N. Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infect Immun. 1983 Jan;39(1):91–99. doi: 10.1128/iai.39.1.91-99.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herring A. J., Inglis N. F., Ojeh C. K., Snodgrass D. R., Menzies J. D. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol. 1982 Sep;16(3):473–477. doi: 10.1128/jcm.16.3.473-477.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hieber J. P., Shelton S., Nelson J. D., Leon J., Mohs E. Comparison of human rotavirus disease in tropical and temperate settings. Am J Dis Child. 1978 Sep;132(9):853–858. doi: 10.1001/archpedi.1978.02120340029004. [DOI] [PubMed] [Google Scholar]
  9. Kalica A. R., Greenberg H. B., Espejo R. T., Flores J., Wyatt R. G., Kapikian A. Z., Chanock R. M. Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2. Infect Immun. 1981 Sep;33(3):958–961. doi: 10.1128/iai.33.3.958-961.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology. 1981 Jul 30;112(2):385–390. doi: 10.1016/0042-6822(81)90285-3. [DOI] [PubMed] [Google Scholar]
  11. Kapikian A. Z., Cline W. L., Greenberg H. B., Wyatt R. G., Kalica A. R., Banks C. E., James H. D., Jr, Flores J., Chanock R. M. Antigenic characterization of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA): evidence for distinctness of IAHA and neutralization antigens. Infect Immun. 1981 Aug;33(2):415–425. doi: 10.1128/iai.33.2.415-425.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Lindenschmidt E. G., Siemens H. J., Beck S., Schwartz T., Grüttner R. G., Laufs R. Vergleichende Untersuchungen über das Rotavirussyndrom nach Infektion mit Humanrotavirus der Subgruppe 1 oder 2. Monatsschr Kinderheilkd. 1984 Sep;132(9):654–660. [PubMed] [Google Scholar]
  14. Matsuno S., Hasegawa A., Mukoyama A., Inouye S. A candidate for a new serotype of human rotavirus. J Virol. 1985 May;54(2):623–624. doi: 10.1128/jvi.54.2.623-624.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rodger S. M., Bishop R. F., Birch C., McLean B., Holmes I. H. Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. J Clin Microbiol. 1981 Feb;13(2):272–278. doi: 10.1128/jcm.13.2.272-278.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rodriguez W. J., Kim H. W., Arrobio J. O., Brandt C. D., Chanock R. M., Kapikian A. Z., Wyatt R. G., Parrott R. H. Clinical features of acute gastroenteritis associated with human reovirus-like agent in infants and young children. J Pediatr. 1977 Aug;91(2):188–193. doi: 10.1016/S0022-3476(77)80810-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schnagl R. D., Rodger S. M., Holmes I. H. Variation in human rotavirus electropherotypes occurring between rotavirus gastroenteritis epidemics in central Australia. Infect Immun. 1981 Jul;33(1):17–21. doi: 10.1128/iai.33.1.17-21.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Spencer E., Avendaño F., Araya M. Characteristics and analysis of electropherotypes of human rotavirus isolated in Chile. J Infect Dis. 1983 Jul;148(1):41–48. doi: 10.1093/infdis/148.1.41. [DOI] [PubMed] [Google Scholar]
  19. Thouless M. E., Beards G. M., Flewett T. H. Serotyping and subgrouping of rotavirus strains by the ELISA test. Arch Virol. 1982;73(3-4):219–230. doi: 10.1007/BF01318076. [DOI] [PubMed] [Google Scholar]
  20. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Bridger J. C., Snodgrass D. R., Herring J. A. Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Arch Virol. 1977;53(4):287–294. doi: 10.1007/BF01315627. [DOI] [PubMed] [Google Scholar]
  21. Vesikari T., Isolauri E., Delem A., D'Hondt E., André F. E., Zissis G. Immunogenicity and safety of live oral attenuated bovine rotavirus vaccine strain RIT 4237 in adults and young children. Lancet. 1983 Oct 8;2(8354):807–811. doi: 10.1016/s0140-6736(83)90734-1. [DOI] [PubMed] [Google Scholar]
  22. White L., Perez I., Perez M., Urbina G., Greenberg H., Kapikian A., Flores J. Relative frequency of rotavirus subgroups 1 and 2 in Venezuelan children with gastroenteritis as assayed with monoclonal antibodies. J Clin Microbiol. 1984 Apr;19(4):516–520. doi: 10.1128/jcm.19.4.516-520.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wyatt R. G., James H. D., Jr, Pittman A. L., Hoshino Y., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. J Clin Microbiol. 1983 Aug;18(2):310–317. doi: 10.1128/jcm.18.2.310-317.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yolken R. H., Wyatt R. G., Zissis G., Brandt C. D., Rodriguez W. J., Kim H. W., Parrott R. H., Urrutia J. J., Mata L., Greenberg H. B. Epidemiology of human rotavirus Types 1 and 2 as studied by enzyme-linked immunosorbent assay. N Engl J Med. 1978 Nov 23;299(21):1156–1161. doi: 10.1056/NEJM197811232992103. [DOI] [PubMed] [Google Scholar]

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