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. 1986 Mar;23(3):643–646. doi: 10.1128/jcm.23.3.643-646.1986

Major outer membrane protein of Legionella pneumophila carries a species-specific epitope.

F S Nolte, C A Conlin
PMCID: PMC268715  PMID: 2420824

Abstract

A monoclonal antibody (LP3IIG2) directed against a species-specific epitope of Legionella pneumophila is available from Genetic Systems Corp., Seattle, Wash., for use as a diagnostic reagent. Outer membrane protein-rich fractions were prepared from L. pneumophila serogroups 1 to 8 by treatment of cell envelopes with 2% Triton X-100. Immunoblots of sodium dodecyl sulfate-polyacrylamide gels demonstrated that each membrane fraction contained two bands that reacted with LP3IIG2. The monoclonal antibody bound preferentially to a 26,000-molecular-weight band that appears to result from modification of the 29,000-molecular-weight major outer membrane protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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