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. 1986 May;23(5):822–825. doi: 10.1128/jcm.23.5.822-825.1986

Determination of mycobacterial antigens in sputum by enzyme immunoassay.

M A Yáñez, M P Coppola, D A Russo, E Delaha, S D Chaparas, H Yeager Jr
PMCID: PMC268729  PMID: 3086369

Abstract

An enzyme-linked immunosorbent assay (ELISA) was examined for its usefulness in detecting mycobacterial antigens in sputum. A double-antibody sandwich procedure was set up by using a commercially available hyperimmune serum directed against Mycobacterium bovis, BCG. The ELISA was able to detect 10 ng of protein per ml of BCG sonic extract. The system also clearly distinguished Mycobacterium tuberculosis organisms from Mycobacterium avium and Mycobacterium kansasii organisms. A total of 68 unknown sputum specimens submitted to the clinical laboratories for examination for tuberculosis were tested by ELISA. Of the 20 specimens that were smear positive and culture positive, 12 (60%) were positive by ELISA; 6 of the 11 (55%) smear-positive culture-negative samples were positive by ELISA; 1 of 2 (50%) of the smear-negative culture-positive samples was positive by ELISA; and only 3 of 35 (9%) of the smear-negative culture-negative samples were positive by ELISA. This approach offers promise as an aid in the presumptive differentiation of nontuberculous mycobacteria from the M. tuberculosis complex.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benjamin R. G., Daniel T. M. Serodiagnosis of tuberculosis using the enzyme-linked immunoabsorbent assay (ELISA) of antibody to Mycobacterium tuberculosis antigen 5. Am Rev Respir Dis. 1982 Dec;126(6):1013–1016. doi: 10.1164/arrd.1982.126.6.1013. [DOI] [PubMed] [Google Scholar]
  2. Brostoff J., Lenzini L., Rottoli P., Rottoli L. Immune complexes in the spectrum of tuberculosis. Tubercle. 1981 Sep;62(3):169–173. doi: 10.1016/0041-3879(81)90002-7. [DOI] [PubMed] [Google Scholar]
  3. Carr R. I., Chakraborty A. K., Brunda M. J., Davidson P. T., Damle P. B., Hardtke M. A., Gilbride K. J., Minden P. Immune complexes and antibodies to BCG in sera from patients with mycobacterial infections. Clin Exp Immunol. 1980 Mar;39(3):562–569. [PMC free article] [PubMed] [Google Scholar]
  4. Chaparas S. D., Hedrick S. R. Comparison of strains of BCG. I. Antigenic analysis and tuberculin reactivity. Infect Immun. 1973 May;7(5):777–780. doi: 10.1128/iai.7.5.777-780.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grange J. M. The humoral immune response in tuberculosis: its nature, biological role and diagnostic usefulness. Adv Tuberc Res. 1984;21:1–78. [PubMed] [Google Scholar]
  6. Harboe M., Mshana R. N., Closs O., Kronvall G., Axelsen N. H. Cross-reactions between mycobacteria. II. Crossed immunoelectrophoretic analysis of soluble antigens of BCG and comparison with other mycobacteria. Scand J Immunol. 1979;9(2):115–124. doi: 10.1111/j.1365-3083.1979.tb02713.x. [DOI] [PubMed] [Google Scholar]
  7. Kalish S. B., Radin R. C., Phair J. P., Levitz D., Zeiss C. R., Metzger E. Use of an enzyme-linked immunosorbent assay technique in the differential diagnosis of active pulmonary tuberculosis in humans. J Infect Dis. 1983 Mar;147(3):523–530. doi: 10.1093/infdis/147.3.523. [DOI] [PubMed] [Google Scholar]
  8. Kim T. C., Blackman R. S., Heatwole K. M., Kim T., Rochester D. F. Acid-fast bacilli in sputum smears of patients with pulmonary tuberculosis. Prevalence and significance of negative smears pretreatment and positive smears post-treatment. Am Rev Respir Dis. 1984 Feb;129(2):264–268. [PubMed] [Google Scholar]
  9. Krambovitis E., McIllmurray M. B., Lock P. E., Hendrickse W., Holzel H. Rapid diagnosis of tuberculous meningitis by latex particle agglutination. Lancet. 1984 Dec 1;2(8414):1229–1231. doi: 10.1016/s0140-6736(84)92792-2. [DOI] [PubMed] [Google Scholar]
  10. Sada E., Ruiz-Palacios G. M., López-Vidal Y., Ponce de León S. Detection of mycobacterial antigens in cerebrospinal fluid of patients with tuberculous meningitis by enzyme-linked immunosorbent assay. Lancet. 1983 Sep 17;2(8351):651–652. doi: 10.1016/s0140-6736(83)92532-1. [DOI] [PubMed] [Google Scholar]
  11. Siddiqi S. H., Hwangbo C. C., Silcox V., Good R. C., Snider D. E., Jr, Middlebrook G. Rapid radiometric methods to detect and differentiate Mycobacterium tuberculosis/M. bovis from other mycobacterial species. Am Rev Respir Dis. 1984 Oct;130(4):634–640. doi: 10.1164/arrd.1984.130.4.634. [DOI] [PubMed] [Google Scholar]
  12. Straus E., Wu N., Quraishi M. A., Levine S. Clinical applications of the radioimmunoassay of secretory tuberculoprotein. Proc Natl Acad Sci U S A. 1981 May;78(5):3214–3217. doi: 10.1073/pnas.78.5.3214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Straus E., Wu N. Radioimmunoassay of tuberculoprotein derived from Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4301–4304. doi: 10.1073/pnas.77.7.4301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Voller A., Bidwell D. E., Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull World Health Organ. 1976;53(1):55–65. [PMC free article] [PubMed] [Google Scholar]
  15. Yolken R. H. Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects. Rev Infect Dis. 1982 Jan-Feb;4(1):35–68. doi: 10.1093/clinids/4.1.35. [DOI] [PubMed] [Google Scholar]
  16. Zeiss C. R., Kalish S. B., Erlich K. S., Levitz D., Metzger E., Radin R., Phair J. P. IgG antibody to purified protein derivative by enzyme-linked immunosorbent assay in the diagnosis of pulmonary tuberculosis. Am Rev Respir Dis. 1984 Nov;130(5):845–848. doi: 10.1164/arrd.1984.130.5.845. [DOI] [PubMed] [Google Scholar]

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