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. 1986 Jun;23(6):1152–1154. doi: 10.1128/jcm.23.6.1152-1154.1986

Surveillance of mice for antibodies to murine cytomegalovirus.

C A Anderson, J C Murphy, J G Fox
PMCID: PMC268815  PMID: 3011855

Abstract

The sera of 256 mice from nine commercial sources were screened for antibodies to murine cytomegalovirus (MCMV) because a surveillance of this virus has not been reported in the literature for over a decade. Although no evidence of antibodies to MCMV were detected by complement fixation or nuclear anticomplement immunofluorescence, 54.7% of these sera did have antibodies that were detected by enzyme-linked immunosorbent assay. These data emphasize the need for proper containment of laboratory mice to prevent the potential outbreak of acute MCMV infection. Including MCMV antibody surveillance by enzyme-linked immunosorbent assay in routine health monitoring of mice and imparting these findings in an analysis of the role of MCMV on interpretation of experimental results is advised.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. A., Murphy J. C., Fox J. G. Evaluation of murine cytomegalovirus antibody detection by serological techniques. J Clin Microbiol. 1983 Oct;18(4):753–758. doi: 10.1128/jcm.18.4.753-758.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRODSKY I., ROWE W. P. Chronic subclinical infection with mouse salivary gland virus. Proc Soc Exp Biol Med. 1958 Dec;99(3):654–655. doi: 10.3181/00379727-99-24451. [DOI] [PubMed] [Google Scholar]
  3. Booss J., Wheelock E. F. Progressive inhibition of T-cell function preceding clinical signs of cytomegalovirus infection in mice. J Infect Dis. 1977 Mar;135(3):478–481. doi: 10.1093/infdis/135.3.478. [DOI] [PubMed] [Google Scholar]
  4. Brautigam A. R., Dutko F. J., Olding L. B., Oldstone M. B. Pathogenesis of murine cytomegalovirus infection: the macrophage as a permissive cell for cytomegalovirus infection, replication and latency. J Gen Virol. 1979 Aug;44(2):349–359. doi: 10.1099/0022-1317-44-2-349. [DOI] [PubMed] [Google Scholar]
  5. Brody A. R., Craighead J. E. Pathogenesis of pulmonary cytomegalovirus infection in immunosuppressed mice. J Infect Dis. 1974 Jun;129(6):677–689. doi: 10.1093/infdis/129.6.677. [DOI] [PubMed] [Google Scholar]
  6. COOK J. E. Salivary-gland virus disease of guinea pigs. J Natl Cancer Inst. 1958 May;20(5):905–909. [PubMed] [Google Scholar]
  7. Collins M. J., Jr, Parker J. C. Murine virus contaminants of leukemia viruses and transplantable tumors. J Natl Cancer Inst. 1972 Oct;49(4):1139–1143. [PubMed] [Google Scholar]
  8. Gardner M. B., Officer J. E., Parker J., Estes J. D., Rongey R. W. Induction of disseminated virulent cytomegalovirus infection by immunosuppression of naturally chronically infected wild mice. Infect Immun. 1974 Oct;10(4):966–969. doi: 10.1128/iai.10.4.966-969.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grundy J. E., Mackenzie J. S., Stanley N. F. Influence of H-2 and non-H-2 genes on resistance to murine cytomegalovirus infection. Infect Immun. 1981 Apr;32(1):277–286. doi: 10.1128/iai.32.1.277-286.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HARTLEY J. W., ROWE W. P., HUEBNER R. J. Serial propagation of the guinea pig salivary gland virus in tissue culture. Proc Soc Exp Biol Med. 1957 Nov;96(2):281–285. doi: 10.3181/00379727-96-23455. [DOI] [PubMed] [Google Scholar]
  11. Jordan M. C. Interstitial pneumonia and subclinical infection after intranasal inoculation of murine cytomegalovirus. Infect Immun. 1978 Jul;21(1):275–280. doi: 10.1128/iai.21.1.275-280.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jordan M. C., Takagi J. L., Stevens J. G. Activation of latent murine cytomegalovirus in vivo and in vitro: a pathogenetic role for acute infection. J Infect Dis. 1982 May;145(5):699–705. doi: 10.1093/infdis/145.2.699. [DOI] [PubMed] [Google Scholar]
  13. MANNINI A., MEDEARIS D. N., Jr Mouse salivary gland virus infections. Am J Hyg. 1961 May;73:329–343. doi: 10.1093/oxfordjournals.aje.a120192. [DOI] [PubMed] [Google Scholar]
  14. Medearis D. N., Jr, Prokay S. L. Effect of immunization of mothers on cytomegalovirus infection in suckling mice. Proc Soc Exp Biol Med. 1978 Apr;157(4):523–527. doi: 10.3181/00379727-157-40089. [DOI] [PubMed] [Google Scholar]
  15. Peters R. L., Collins M. J., O'Beirne A. J., Howton P. A., Hourihan S. L., Thomas S. F. Enzyme-linked immunosorbent assay for detection of antibodies to murine hepatitis virus. J Clin Microbiol. 1979 Oct;10(4):595–597. doi: 10.1128/jcm.10.4.595-597.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shanley J. D., Pesanti E. L., Nugent K. M. The pathogenesis of pneumonitis due to murine cytomegalovirus. J Infect Dis. 1982 Sep;146(3):388–396. doi: 10.1093/infdis/146.3.388. [DOI] [PubMed] [Google Scholar]

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