Abstract
An enzyme-linked immunosorbent assay was done for the detection of immunoglobulin G and M (IgG and IgM) antibodies to Echinococcus granulosus in surgically proved cases of hydatidosis, especially pulmonary hydatidosis, by use of human hydatid cyst fluid antigen and soluble scolex antigen. This assay was compared with the following standardized techniques: the indirect hemagglutination test, the indirect immunofluorescent antibody test, and Casoni's intradermal test. The enzyme-linked immunosorbent assay, with either of the antigens (human hydatid cyst fluid or soluble scolex antigen), was more sensitive and specific than the other techniques in diagnosing cases of hydatidosis, especially hydatid disease of the lung.
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- Afferni C., Pini C., Misiti-Dorello P., Bernardini L., Conchedda M., Vicari G. Detection of specific IgE antibodies in sera from patients with hydatidosis. Clin Exp Immunol. 1984 Mar;55(3):587–592. [PMC free article] [PubMed] [Google Scholar]
- Bombardieri S., Giordano F., Ingrao F., Ioppolo S., Siracusano A., Vicari G. An evaluation of an agar gel diffusion test with crude and purified antigens in the diagnosis of hydatid disease. Bull World Health Organ. 1974;51(5):525–530. [PMC free article] [PubMed] [Google Scholar]
- Chemtai A. K., Bowry T. R., Ahmad Z. Evaluation of five immunodiagnostic techniques in echinococcosis patients. Bull World Health Organ. 1981;59(5):767–772. [PMC free article] [PubMed] [Google Scholar]
- Farag H., Bout D., Capron A. Specific immunodiagnosis of human hydatidosis by the enzyme linked immunosorbent assay (E.L.I.S.A.). Biomedicine. 1975 Sep 10;23(7):276–278. [PubMed] [Google Scholar]
- Iacona A., Pini C., Vicari G. Enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of hydatid disease. Am J Trop Med Hyg. 1980 Jan;29(1):95–102. doi: 10.4269/ajtmh.1980.29.95. [DOI] [PubMed] [Google Scholar]
- Kagan I. G. A review of serological tests for the diagnosis of hydatid disease. Bull World Health Organ. 1968;39(1):25–37. [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Mahajan R. C., Ganguly N. K., Chitkara N. L., Agarwal S. C. Comparative evaluation of indirect haemagglutination intradermal and complement fixation tests in diagnosis of hydatid diseases. Indian J Med Res. 1973 Mar;61(3):335–339. [PubMed] [Google Scholar]
- Mahajan R. C., Ganguly N. K., Chitkara N. L. Evaluation of immunodiagnostic techniques for hydatid disease. Indian J Med Res. 1976 Mar;64(3):405–409. [PubMed] [Google Scholar]
- McLaren M. L., Lillywhite J. E., Au A. C. Indirect enzyme linked immunosorbent assay (ELISA): practical aspects of standardization and quality control. Med Lab Sci. 1981 Jul;38(3):245–251. [PubMed] [Google Scholar]
- Oriol R., Williams J. F., Pérez Esandi M. V., Oriol C. Purification of lipoprotein antigens of Echinococcus granulosus from sheep hydatid fluid. Am J Trop Med Hyg. 1971 Jul;20(4):569–574. doi: 10.4269/ajtmh.1971.20.569. [DOI] [PubMed] [Google Scholar]
- Pini C., Pastore R., Valesini G. Circulating immune complexes in sera of patients infected with Echinococcus granulosus. Clin Exp Immunol. 1983 Mar;51(3):572–578. [PMC free article] [PubMed] [Google Scholar]
- Todorov T., Dakov I., Kosturkova M., Tenev S., Dimitrov A. Immunoreactivity in pulmonary echinococcosis. A comparative study of immunodiagnostic tests. Bull World Health Organ. 1979;57(5):735–740. [PMC free article] [PubMed] [Google Scholar]
- Todorov T., Raicev I., Tenev S., Kosturkova M., Dakov I., Dimitrov A. Immunoreactivity in pulmonary echinococcosis. 2. Evaluation of antibody response. Bull World Health Organ. 1979;57(5):741–750. [PMC free article] [PubMed] [Google Scholar]
- Todorov T., Stojanov G., Rohov L., Rasev R., Alova N. Antibody persistence after surgical treatment of echinococcosis. Bull World Health Organ. 1976;53(4):407–415. [PMC free article] [PubMed] [Google Scholar]
- Voller A., De Savigny D. Diagnostic serology of tropical parasitic diseases. J Immunol Methods. 1981;46(1):1–29. doi: 10.1016/0022-1759(81)90328-8. [DOI] [PubMed] [Google Scholar]
- Wattal C., Malla N., Khan I., Agarwal S. C. In vitro development & hatching of Ascaris lumbricoides var. hominis eggs. Indian J Med Res. 1986 Mar;83:257–264. [PubMed] [Google Scholar]