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. 1986 Jul;24(1):47–51. doi: 10.1128/jcm.24.1.47-51.1986

Evaluation of avian-human reassortant influenza A/Washington/897/80 x A/Pintail/119/79 virus in monkeys and adult volunteers.

M L Clements, M H Snyder, A J Buckler-White, E L Tierney, W T London, B R Murphy
PMCID: PMC268829  PMID: 3722365

Abstract

A reassortant influenza A virus was produced by mating an avian influenza A/Pintail/Alberta/119/79 (H4N6) virus with wild-type human influenza A/Washington/897/80 (H3N2) virus. The avian-human influenza A reassortant virus contained the genes coding for the hemagglutinin and neuraminidase surface antigens of the human influenza wild-type virus and the six other RNA segments (internal genes) of the avian influenza A virus donor. In the lower respiratory tract of squirrel monkeys, this avian-human influenza reassortant virus, like its avian influenza A parent virus, was restricted approximately 100-fold in replication compared with the wild-type human influenza A virus. Despite this restriction of replication, infection of monkeys with the avian-human influenza A reassortant virus induced resistance to wild-type human influenza A virus challenge. In comparison with the wild-type human influenza A virus, the avian-human influenza A reassortant was also fully attenuated when 10(5.5) to 10(7.5) 50% tissue culture infective doses were administered to susceptible adult volunteers. Attenuation was indicated by a more than 300-fold reduction in virus shedding and lack of reactogenicity. The reassortant virus did not spread to susceptible contacts and could not be isolated from the blood or stools of infected adults. The 50% human infectious dose was 10(6.2) 50% tissue culture infective dose, indicating that this reassortant virus is only slightly less infectious for adults than a similarly derived avian-human influenza A/Washington/80 X A/Mallard/78 reassortant virus. These findings suggest that the avian influenza A/Pintail/79 virus may be a satisfactory donor of attenuating genes for production of live, attenuated avian-human influenza A reassortant virus vaccines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aymard-Henry M., Coleman M. T., Dowdle W. R., Laver W. G., Schild G. C., Webster R. G. Influenzavirus neuraminidase and neuraminidase-inhibition test procedures. Bull World Health Organ. 1973;48(2):199–202. [PMC free article] [PubMed] [Google Scholar]
  2. Buckler-White A. J., Naeve C. W., Murphy B. R. Characterization of a gene coding for M proteins which is involved in host range restriction of an avian influenza A virus in monkeys. J Virol. 1986 Feb;57(2):697–700. doi: 10.1128/jvi.57.2.697-700.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clements M. L., Betts R. F., Maassab H. F., Murphy B. R. Dose response of influenza A/Washington/897/80 (H3N2) cold-adapted reassortant virus in adult volunteers. J Infect Dis. 1984 May;149(5):814–815. doi: 10.1093/infdis/149.5.814. [DOI] [PubMed] [Google Scholar]
  4. Murphy B. R., Buckler-White A. J., London W. T., Harper J., Tierney E. L., Miller N. T., Reck L. J., Chanock R. M., Hinshaw V. S. Avian-human reassortant influenza A viruses derived by mating avian and human influenza A viruses. J Infect Dis. 1984 Dec;150(6):841–850. doi: 10.1093/infdis/150.6.841. [DOI] [PubMed] [Google Scholar]
  5. Murphy B. R., Chalhub E. G., Nusinoff S. R., Kasel J., Chanock R. M. Temperature-sensitive mutants of influenza virus. 3. Further characterization of the ts-1(E) influenza A recombinant (H3N2) virus in man. J Infect Dis. 1973 Oct;128(4):479–487. doi: 10.1093/infdis/128.4.479. [DOI] [PubMed] [Google Scholar]
  6. Murphy B. R., Clements M. L., Tierney E. L., Black R. E., Stienberg J., Chanock R. M. Dose response of influenza A/Washington/897/80 (H3N2) avian-human reassortant virus in adult volunteers. J Infect Dis. 1985 Jul;152(1):225–229. doi: 10.1093/infdis/152.1.225. [DOI] [PubMed] [Google Scholar]
  7. Murphy B. R., Hinshaw V. S., Sly D. L., London W. T., Hosier N. T., Wood F. T., Webster R. G., Chanock R. M. Virulence of avian influenza A viruses for squirrel monkeys. Infect Immun. 1982 Sep;37(3):1119–1126. doi: 10.1128/iai.37.3.1119-1126.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Murphy B. R., Phelan M. A., Nelson D. L., Yarchoan R., Tierney E. L., Alling D. W., Chanock R. M. Hemagglutinin-specific enzyme-linked immunosorbent assay for antibodies to influenza A and B viruses. J Clin Microbiol. 1981 Mar;13(3):554–560. doi: 10.1128/jcm.13.3.554-560.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murphy B. R., Sly D. L., Hosier N. T., London W. T., Chanock R. M. Evaluation of three strains of influenza A virus in humans and in owl, cebus, and squirrel monkeys. Infect Immun. 1980 Jun;28(3):688–691. doi: 10.1128/iai.28.3.688-691.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Murphy B. R., Sly D. L., Tierney E. L., Hosier N. T., Massicot J. G., London W. T., Chanock R. M., Webster R. G., Hinshaw V. S. Reassortant virus derived from avian and human influenza A viruses is attenuated and immunogenic in monkeys. Science. 1982 Dec 24;218(4579):1330–1332. doi: 10.1126/science.6183749. [DOI] [PubMed] [Google Scholar]
  11. Richman D. D., Murphy B. R., Cline W. L., Alling D. W. Determination of influenzavirus neuraminidase inhibition titres. Bull World Health Organ. 1975;52(2):233–234. [PMC free article] [PubMed] [Google Scholar]
  12. Scholtissek C., von Hoyningen-Huene V. Genetic relatedness of the gene which codes for the nonstructural (NS) protein of different influenza A strains. Virology. 1980 Apr 15;102(1):13–20. doi: 10.1016/0042-6822(80)90065-3. [DOI] [PubMed] [Google Scholar]
  13. Snyder M. H., Clements M. L., Betts R. F., Dolin R., Buckler-White A. J., Tierney E. L., Murphy B. R. Evaluation of live avian-human reassortant influenza A H3N2 and H1N1 virus vaccines in seronegative adult volunteers. J Clin Microbiol. 1986 May;23(5):852–857. doi: 10.1128/jcm.23.5.852-857.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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