Abstract
The antigens from immune complexes of sera from patients with mycobacterial diseases were released by sodium dodecyl sulfate. The antigenic activity of the released proteins was tested by agar gel diffusion and immunoelectrophoresis. This simple method provided direct evidence for the presence of mycobacterial antigens in the immune complexes of sera from patients with leprosy and tuberculosis.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chakrabarty A. K., Maire M., Saha K., Lambert P. H. Identification of components of IC purified from human sera. II. Demonstration of mycobacterial antigens in immune complexes isolated from sera of lepromatous patients. Clin Exp Immunol. 1983 Feb;51(2):225–231. [PMC free article] [PubMed] [Google Scholar]
- Cho S. N., Yanagihara D. L., Hunter S. W., Gelber R. H., Brennan P. J. Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy. Infect Immun. 1983 Sep;41(3):1077–1083. doi: 10.1128/iai.41.3.1077-1083.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GIVOL D., FUCHS S., SELA M. Isolation of antibodies to antigens of low molecular weight. Biochim Biophys Acta. 1962 Sep 10;63:222–224. doi: 10.1016/0006-3002(62)90362-1. [DOI] [PubMed] [Google Scholar]
- Harboe N., Ingild A. Immunization, isolation of immunoglobulins, estimation of antibody titre. Scand J Immunol Suppl. 1973;1:161–164. doi: 10.1111/j.1365-3083.1973.tb03798.x. [DOI] [PubMed] [Google Scholar]
- Höffken K., Bosse F., Steih U., Schmidt C. G. Dissociation and isolation of antigen and antibody from immune complexes. J Immunol Methods. 1982 Aug 27;53(1):51–59. doi: 10.1016/0022-1759(82)90239-3. [DOI] [PubMed] [Google Scholar]
- Lenzini L., Rottoli P., Rottoli L. The spectrum of human tuberculosis. Clin Exp Immunol. 1977 Feb;27(2):230–237. [PMC free article] [PubMed] [Google Scholar]
- Maidment B. W., Jr, Papsidero L. D., Chu T. M. Isoelectric focusing--a new approach to the study of immune complexes. J Immunol Methods. 1980;35(3-4):297–306. doi: 10.1016/0022-1759(80)90256-2. [DOI] [PubMed] [Google Scholar]
- Maidment B. W., Jr, Papsidero L. D., Nemoto T., Chu T. M. Recovery of immunologically reactive antibodies and antigens from breast cancer immune complexes by preparative isoelectric focusing. Cancer Res. 1981 Mar;41(3):795–800. [PubMed] [Google Scholar]
- Mehra V., Brennan P. J., Rada E., Convit J., Bloom B. R. Lymphocyte suppression in leprosy induced by unique M. leprae glycolipid. Nature. 1984 Mar 8;308(5955):194–196. doi: 10.1038/308194a0. [DOI] [PubMed] [Google Scholar]
- Ramanathan V. D., Parkash O., Ramu G., Parker D., Curtis J., Sengupta U., Turk J. L. Isolation and analysis of circulating immune complexes in leprosy. Clin Immunol Immunopathol. 1984 Sep;32(3):261–268. doi: 10.1016/0090-1229(84)90270-8. [DOI] [PubMed] [Google Scholar]
- Ridley D. S., Jopling W. H. Classification of leprosy according to immunity. A five-group system. Int J Lepr Other Mycobact Dis. 1966 Jul-Sep;34(3):255–273. [PubMed] [Google Scholar]
- Sinha S., Sengupta U., Ramu G., Ivanyi J. Serological survey of leprosy and control subjects by a monoclonal antibody-based immunoassay. Int J Lepr Other Mycobact Dis. 1985 Mar;53(1):33–38. [PubMed] [Google Scholar]
- Slobin L. I., Sela M. Use of urea in the purification of antibodies. Biochim Biophys Acta. 1965 Oct 18;107(3):593–596. doi: 10.1016/0304-4165(65)90202-3. [DOI] [PubMed] [Google Scholar]
- Theofilopoulos A. N., Dixon F. J. The biology and detection of immune complexes. Adv Immunol. 1979;28:89–220. doi: 10.1016/s0065-2776(08)60800-7. [DOI] [PubMed] [Google Scholar]
- Wadee A. A., Mendelsohn D., Rabson A. R. Characterization of a suppressor cell-activating factor (SCAF) released by adherent cells treated with M. tuberculosis. J Immunol. 1983 May;130(5):2266–2270. [PubMed] [Google Scholar]