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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Aug;24(2):173–176. doi: 10.1128/jcm.24.2.173-176.1986

Comparative evaluation of identification systems for testing methicillin-resistant strains of Staphylococcus aureus.

S M Smith, C Berezny
PMCID: PMC268869  PMID: 3638308

Abstract

Several commercial systems are available to distinguish between Staphylococcus aureus and the coagulase-negative species of the Micrococcaceae family. Four latex agglutination systems (Accu-Staph, SeroSTAT, Staphaurex, and Staphylatex) and two hemagglutination systems (Hemastaph and Staphyloslide) were compared for their performance in the rapid identification of 232 isolates of staphylococci, including 114 of methicillin-resistant S. aureus. Accu-Staph, Staphaurex, and Staphyloslide correctly identified 100% of the methicillin-resistant S. aureus isolates; Hemastaph and Staphylatex, 99.1%; and SeroSTAT, 94.7%. Most reactions were easy to interpret, although 15% of the SeroSTAT reactions were weak. Autoagglutination occurred only with isolates of coagulase-negative staphylococci. False-positive reactions were rare and occurred only with systems which did not detect autoagglutination. Five of these six systems appear to be adequate for the rapid identification of S. aureus, including methicillin-resistant isolates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge K. E., Kogos C., Sanders C. V., Marier R. L. Comparison of rapid identification assays for Staphylococcus aureus. J Clin Microbiol. 1984 May;19(5):703–704. doi: 10.1128/jcm.19.5.703-704.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker J. S., Bormann M. A., Boudreau D. H. Evaluation of various rapid agglutination methods for the identification of Staphylococcus aureus. J Clin Microbiol. 1985 May;21(5):726–729. doi: 10.1128/jcm.21.5.726-729.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doern G. V. Evaluation of a commercial latex agglutination test for identification of Staphylococcus aureus. J Clin Microbiol. 1982 Mar;15(3):416–418. doi: 10.1128/jcm.15.3.416-418.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Doern G. V., Robbie L. I. Direct identification of Staphylococcus aureus in blood culture fluid with a commercial latex agglutination test. J Clin Microbiol. 1982 Dec;16(6):1048–1051. doi: 10.1128/jcm.16.6.1048-1051.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Essers L., Radebold K. Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test. J Clin Microbiol. 1980 Nov;12(5):641–643. doi: 10.1128/jcm.12.5.641-643.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flandrois J. P., Carret G. Study of the staphylococcal affinity to fibrinogen by passive hemagglutination: a tool for the Staphylococcus aureus identification. Zentralbl Bakteriol Mikrobiol Hyg A. 1981 Dec;251(2):171–176. [PubMed] [Google Scholar]
  7. Jungkind D. L., Torhan N. J., Corman K. E., Bondi J. M. Comparison of two commercially available test methods with conventional coagulase tests for identification of Staphylococcus aureus. J Clin Microbiol. 1984 Feb;19(2):191–193. doi: 10.1128/jcm.19.2.191-193.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Maxim P. E., Mathews H. L., Mengoli H. F. Single-tube mixed agglutination test for the detection of staphylococcal protein A. J Clin Microbiol. 1976 Nov;4(5):418–422. doi: 10.1128/jcm.4.5.418-422.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Menzies R. E. Comparison of coagulase, deoxyribonuclease (DNase), and heat-stable nuclease tests for identification of Staphylococcus aureus. J Clin Pathol. 1977 Jul;30(7):606–608. doi: 10.1136/jcp.30.7.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Myrick B. A., Ellner P. D. Evaluation of the latex slide agglutination test for identification of Staphylococcus aureus. J Clin Microbiol. 1982 Feb;15(2):275–277. doi: 10.1128/jcm.15.2.275-277.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pennell D. R., Rott-Petri J. A., Kurzynski T. A. Evaluation of three commercial agglutination tests for the identification of Staphylococcus aureus. J Clin Microbiol. 1984 Oct;20(4):614–617. doi: 10.1128/jcm.20.4.614-617.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith S. M. In vitro comparison of A-56619, A-56620, amifloxacin, ciprofloxacin, enoxacin, norfloxacin, and ofloxacin against methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1986 Feb;29(2):325–326. doi: 10.1128/aac.29.2.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wegrzynowicz Z., Heczko P. B., Jeljaszewicz J., Neugebauer M., Pulverer G. Pseudocoagulase activity of staphylococci. J Clin Microbiol. 1979 Jan;9(1):15–19. doi: 10.1128/jcm.9.1.15-19.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Winblad S., Ericson C. Sensitized sheep red cells as a reactant for Staphylococcus aureus protein A. Methodology and epidemiology with special reference to weakly reacting methicillin-resistant strains. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Feb;81(1):150–156. [PubMed] [Google Scholar]
  15. Zarzour J. Y., Belle E. A. Evaluation of three test procedures for identification of Staphylococcus aureus from clinical sources. J Clin Microbiol. 1978 Feb;7(2):133–136. doi: 10.1128/jcm.7.2.133-136.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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