Abstract
A study designed to screen for the presence of methicillin-resistant Staphylococcus aureus from primary plates was conducted from 1 January to 1 September 1985 in a small community hospital. The screening method used a plate of lipovitellin salt mannitol agar and a 4-microgram oxacillin disk incubated at 30 degrees C. Growth of yellow colonies, typical of S. aureus, around the disk without a zone of inhibition was called presumptive methicillin-resistant S. aureus. All susceptibilities were confirmed by using the National Committee for Clinical Laboratory Standards macrodilution technique. Of 224 cultures containing S. aureus, 118 (53%) were positive for methicillin-resistant S. aureus isolates. Of these 118, 111 (94%) were correctly identified from the primary plates as methicillin-resistant S. aureus. Of the 224 isolates, 14 could not be categorized from the primary plates as methicillin-resistant S. aureus due to the small amounts of S. aureus recovered.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Archer G. L., Mayhall C. G. Comparison of epidemiological markers used in the investigation of an outbreak of methicillin-resistant Staphylococcus aureus infections. J Clin Microbiol. 1983 Aug;18(2):395–399. doi: 10.1128/jcm.18.2.395-399.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BARBER M. Methicillin-resistant staphylococci. J Clin Pathol. 1961 Jul;14:385–393. doi: 10.1136/jcp.14.4.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Churcher G. M. A screening test for the detection of methicillin-resistant staphylococci. J Clin Pathol. 1968 Mar;21(2):213–217. doi: 10.1136/jcp.21.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gunn B. A., Dunkelberg W. E., Jr, Creitz J. R. Clinical evaluation of 2 -LSM medium for primary isolation and identification of staphylococci. Am J Clin Pathol. 1972 Feb;57(2):236–240. doi: 10.1093/ajcp/57.2.236. [DOI] [PubMed] [Google Scholar]
- Haley R. W., Hightower A. W., Khabbaz R. F., Thornsberry C., Martone W. J., Allen J. R., Hughes J. M. The emergence of methicillin-resistant Staphylococcus aureus infections in United States hospitals. Possible role of the house staff-patient transfer circuit. Ann Intern Med. 1982 Sep;97(3):297–308. doi: 10.7326/0003-4819-97-3-297. [DOI] [PubMed] [Google Scholar]
- Hansen S. L., Freedy P. K. Variation in the abilities of automated, commercial, and reference methods to detect methicillin-resistant (heteroresistant) Staphylococcus aureus. J Clin Microbiol. 1984 Sep;20(3):494–499. doi: 10.1128/jcm.20.3.494-499.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartman B. J., Tomasz A. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus. J Bacteriol. 1984 May;158(2):513–516. doi: 10.1128/jb.158.2.513-516.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lally R. T., Ederer M. N., Woolfrey B. F. Evaluation of mannitol salt agar with oxacillin as a screening medium for methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 1985 Oct;22(4):501–504. doi: 10.1128/jcm.22.4.501-504.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lyon B. R., May J. W., Marshall J. H., Skurray R. A. Plasmid-mediated antibiotic resistance in methicillin-resistant Staphylococcus aureus. Med J Aust. 1982 May 29;1(11):468–469. doi: 10.5694/j.1326-5377.1982.tb132418.x. [DOI] [PubMed] [Google Scholar]
- McDougal L. K., Thornsberry C. New recommendations for disk diffusion antimicrobial susceptibility tests for methicillin-resistant (heteroresistant) staphylococci. J Clin Microbiol. 1984 Apr;19(4):482–488. doi: 10.1128/jcm.19.4.482-488.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sabath L. D. Mechanisms of resistance to beta-lactam antibiotics in strains of Staphylococcus aureus. Ann Intern Med. 1982 Sep;97(3):339–344. doi: 10.7326/0003-4819-97-3-339. [DOI] [PubMed] [Google Scholar]
- Saravolatz L. D., Pohlod D. J., Arking L. M. Community-acquired methicillin-resistant Staphylococcus aureus infections: a new source for nosocomial outbreaks. Ann Intern Med. 1982 Sep;97(3):325–329. doi: 10.7326/0003-4819-97-3-325. [DOI] [PubMed] [Google Scholar]
- Schaefler S., Jones D., Perry W., Baradet T., Mayr E., Rampersad C. Methicillin-resistant Staphylococcus aureus strains in New York City hospitals: inter-hospital spread of resistant strains of type 88. J Clin Microbiol. 1984 Sep;20(3):536–538. doi: 10.1128/jcm.20.3.536-538.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thornsberry C., Caruthers J. Q., Baker C. N. Effect of temperature on the in vitro susceptibility of Staphylococcus aureus to penicillinase-resistant penicillins. Antimicrob Agents Chemother. 1973 Sep;4(3):263–269. doi: 10.1128/aac.4.3.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thornsberry C., McDougal L. K. Successful use of broth microdilution in susceptibility tests for methicillin-resistant (heteroresistant) staphylococci. J Clin Microbiol. 1983 Nov;18(5):1084–1091. doi: 10.1128/jcm.18.5.1084-1091.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Welch W. D., Southern P. M. Unusual susceptibility of methicillin-resistant Staphylococcus aureus to erythromycin, clindamycin, gentamicin, and tetracycline at 30 degrees C but not at 35 degrees C. J Clin Microbiol. 1984 Jun;19(6):831–833. doi: 10.1128/jcm.19.6.831-833.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]