Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Aug;24(2):228–232. doi: 10.1128/jcm.24.2.228-232.1986

Purification and characterization of an Aeromonas hydrophila hemolysin.

T Asao, S Kozaki, K Kato, Y Kinoshita, K Otsu, T Uemura, G Sakaguchi
PMCID: PMC268880  PMID: 3745420

Abstract

A hemolysin produced by Aeromonas hydrophila CA-11, isolated from an environmental source, was purified by sulfopropyl-Sephadex C-25 chromatography at pH 5.0. This hemolysin caused fluid accumulation in infant mouse intestines and rabbit intestinal loops and killed Vero cells, as did the hemolysin produced by strain AH-1, isolated from a diarrheal case. In polyacrylamide gel electrophoreses at pHs 4.0 and 9.4 and in thin-layer isoelectric focusing, CA-11 hemolysin migrated as a single band to a position different from that of AH-1 hemolysin. Immunodiffusion tests indicated that CA-11 hemolysin was immunologically related to AH-1 hemolysin but possessed unique antigenic determinants. Neutralization tests with antihemolysin sera also demonstrated immunological cross-reactivity between AH-1 and CA-11 hemolysins. These results apparently indicate that the hemolysins produced by the two strains of A. hydrophila are immunologically and physicochemically different from each other.

Full text

PDF
228

Images in this article

230Image
on p.230
231Image
on p.231

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Annapurna E., Sanyal S. C. Enterotoxicity of Aeromonas hydrophila. J Med Microbiol. 1977 Aug;10(3):317–323. doi: 10.1099/00222615-10-3-317. [DOI] [PubMed] [Google Scholar]
  2. Asao T., Kinoshita Y., Kozaki S., Uemura T., Sakaguchi G. Purification and some properties of Aeromonas hydrophila hemolysin. Infect Immun. 1984 Oct;46(1):122–127. doi: 10.1128/iai.46.1.122-127.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burke V., Gracey M., Robinson J., Peck D., Beaman J., Bundell C. The microbiology of childhood gastroenteritis: Aeromonas species and other infective agents. J Infect Dis. 1983 Jul;148(1):68–74. doi: 10.1093/infdis/148.1.68. [DOI] [PubMed] [Google Scholar]
  4. Burke V., Robinson J., Gracey M., Peterson D., Meyer N., Haley V. Isolation of Aeromonas spp. from an unchlorinated domestic water supply. Appl Environ Microbiol. 1984 Aug;48(2):367–370. doi: 10.1128/aem.48.2.367-370.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cumberbatch N., Gurwith M. J., Langston C., Sack R. B., Brunton J. L. Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrheal disease. Infect Immun. 1979 Mar;23(3):829–837. doi: 10.1128/iai.23.3.829-837.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  7. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  8. Donta S. T., Haddow A. D. Cytotoxic activity of Aeromonas hydrophila. Infect Immun. 1978 Sep;21(3):989–993. doi: 10.1128/iai.21.3.989-993.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hostacká A., Ciznár I., Korych B., Karolcek J. Toxic factors of Aeromonas hydrophila and Plesiomonas shigelloides. Zentralbl Bakteriol Mikrobiol Hyg A. 1982 Sep;252(4):525–534. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Ljungh A., Wadström T. Aeromonas toxins. Pharmacol Ther. 1981;15(3):339–354. doi: 10.1016/0163-7258(81)90049-8. [DOI] [PubMed] [Google Scholar]
  12. Pitarangsi C., Echeverria P., Whitmire R., Tirapat C., Formal S., Dammin G. J., Tingtalapong M. Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides: prevalence among individuals with and without diarrhea in Thailand. Infect Immun. 1982 Feb;35(2):666–673. doi: 10.1128/iai.35.2.666-673.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
  14. Riddle L. M., Graham T. E., Amborski R. L. Medium for the accumulation of extracellular hemolysin and protease by Aeromonas hydrophila. Infect Immun. 1981 Sep;33(3):728–733. doi: 10.1128/iai.33.3.728-733.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Turnbull P. C., Lee J. V., Miliotis M. D., Van de Walle S., Koornhof H. J., Jeffery L., Bryant T. N. Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species. J Clin Microbiol. 1984 Feb;19(2):175–180. doi: 10.1128/jcm.19.2.175-180.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  17. Wretlind B., Hedén L., Wadström T. Formation of extracellular haemolysin by Aeromonas hydrophila in relation to protease and staphylolytic enzyme. J Gen Microbiol. 1973 Sep;78(1):57–65. doi: 10.1099/00221287-78-1-57. [DOI] [PubMed] [Google Scholar]
  18. Wretlind B., Möllby R., Wadström T. Separation of two hemolysins from Aeromonas hydrophila by isoelectric focusing. Infect Immun. 1971 Oct;4(4):503–505. doi: 10.1128/iai.4.4.503-505.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES