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. 1986 Aug;24(2):272–274. doi: 10.1128/jcm.24.2.272-274.1986

Identification of Yersinia enterocolitica O15 lipopolysaccharide as a rough antigen.

Y Kawaoka, G Wauters, K Otsuki, M Tsubokura
PMCID: PMC268888  PMID: 2427540

Abstract

Lipopolysaccharide (LPS) of a reference strain of Yersinia enterocolitica serovar O15, Ye614, was examined for its chemical structure and antigenicity. The O15 LPS was partially hydrolyzed with diluted acetic acid and fractionated on a Sephadex G-50 column. Only one peak was detected at fractions where the R-core oligosaccharide of a Y. enterocolitica rough strain was eluted. The chemical compositions of this oligosaccharide and the O15 LPS were similar to those of the R-core oligosaccharide and the rough LPS, respectively. The O15 LPS was antigenically identical to the rough LPS in immunodiffusion tests. These results indicate that the O15 LPS of Y. enterocolitica is rough LPS and that antigenic factor O15 is a rough antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldová E., Skorkovský B., Kapinus J., Pejhovská M., Soukupová G. On the ecology of Yersinia enterocolitica O 3. Yersinia in synanthropic animals. Zentralbl Bakteriol A. 1980 Mar;246(3):344–352. [PubMed] [Google Scholar]
  2. Aldová E., Sobotková J., Brezinová A., Cerná J., Janecková M., Pegrímková J., Pokorná V. Yersinia enterocolitica in water and foods. Zentralbl Bakteriol Mikrobiol Hyg B. 1981 Sep;173(6):464–470. [PubMed] [Google Scholar]
  3. DAVIES D. A., MORGAN W. T., RECORD B. R. Studies in immunochemistry. 15. The specific polysaccharide of the dominant 'O' somatic antigen of Shigella dysenteriae. Biochem J. 1955 Jun;60(2):290–303. doi: 10.1042/bj0600290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Galanos C., Lüderitz O., Westphal O. A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969 Jun;9(2):245–249. doi: 10.1111/j.1432-1033.1969.tb00601.x. [DOI] [PubMed] [Google Scholar]
  5. Kanazawa Y., Ikemura K. Isolation of Yersinia enterocolitica and Yersinia pseudotuberculosis from human specimens and their drug-resistance in the Niigata District of Japan. Contrib Microbiol Immunol. 1979;5:106–114. [PubMed] [Google Scholar]
  6. Kapperud G. Yersinia enterocolitica and Yersinia like microbes isolated from mammals and water in Norway and Denmark. Acta Pathol Microbiol Scand B. 1977 Apr;85(2):129–135. doi: 10.1111/j.1699-0463.1977.tb01686.x. [DOI] [PubMed] [Google Scholar]
  7. Kawaoka Y., Naiki M., Yanagawa R. Isolation of the antigen-active components from leptospiral serovar-specific lipopolysaccharide antigen by mild acid hydrolysis. Nihon Juigaku Zasshi. 1982 Jun;44(3):473–478. doi: 10.1292/jvms1939.44.473. [DOI] [PubMed] [Google Scholar]
  8. Kawaoka Y., Naiki M., Yanagawa R. Radioimmunoassay system using a serovar-specific lipopolysaccharide antigen of Leptospira. J Clin Microbiol. 1979 Sep;10(3):313–316. doi: 10.1128/jcm.10.3.313-316.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kawaoka Y., Otsuki K., Tsubokura M. Growth temperature-dependent variation in the bacteriophage-inactivating capacity and antigenicity of Yersinia enterocolitica lipopolysaccharide. J Gen Microbiol. 1983 Sep;129(9):2739–2747. doi: 10.1099/00221287-129-9-2739. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Schiemann D. A. Enrichment methods for recovery of Yersinia enterocolitica from foods and raw milk. Contrib Microbiol Immunol. 1979;5:212–227. [PubMed] [Google Scholar]
  12. Tsubokura M., Otsuki K., Shimohira I., Yamamoto H. Production of indirect hemolysin by Yersinia enterocolitica and its properties. Infect Immun. 1979 Sep;25(3):939–942. doi: 10.1128/iai.25.3.939-942.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WEISSBACH A., HURWITZ J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J Biol Chem. 1959 Apr;234(4):705–709. [PubMed] [Google Scholar]
  14. Wauters G., Le Minor L., Chalon A. M., Lassen J. Supplément au schéma antigénique de "Yersinia enterocolitica". Ann Inst Pasteur (Paris) 1972 May;122(5):951–956. [PubMed] [Google Scholar]
  15. Weber G., Stanek G., Massiczek N., Klenner M. F. Yersinia enterocolitica im Trinkwasser. Zentralbl Bakteriol Mikrobiol Hyg B. 1981;173(3-4):209–216. [PubMed] [Google Scholar]

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