Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Aug;24(2):298–300. doi: 10.1128/jcm.24.2.298-300.1986

Competitive enzyme-linked immunosorbent assay for cholera-related enterotoxins in Salmonella typhimurium.

H Hariharan, B A Booth, T J Brickman, W C Katt, M Boesman-Finkelstein, R A Finkelstein
PMCID: PMC268894  PMID: 3528215

Abstract

We report a rapid competitive enzyme-linked immunosorbent assay to screen Salmonella typhimurium strains for cholera-related enterotoxin antigens. Polymyxin B extracts of bacterial cells from syncase-glucose broth cultures of 7 of 15 strains gave positive results. The specificity of the test was confirmed with known heat-labile-enterotoxin-positive and -negative Escherichia coli strains which gave significantly different values.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boesman-Finkelstein M., Finkelstein R. A. Protection in rabbits induced by the Texas Star-SR attenuated A-B+ mutant candidate live oral cholera vaccine. Infect Immun. 1982 Apr;36(1):221–226. doi: 10.1128/iai.36.1.221-226.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Caprioli A., D'Agnolo G., Falbo V., Roda L. G., Tomasi M. Isolation of Salmonella wien heat-labile enterotoxin. Microbiologica. 1982 Jan;5(1):1–10. [PubMed] [Google Scholar]
  3. Duebbert I. E., Peterson J. W. Enterotoxin-induced fluid accumulation during experimental salmonellosis and cholera: involvement of prostaglandin synthesis by intestinal cells. Toxicon. 1985;23(1):157–172. doi: 10.1016/0041-0101(85)90118-7. [DOI] [PubMed] [Google Scholar]
  4. Finkelstein R. A., Atthasampunna P., Chulasamaya M., Charunmethee P. Pathogenesis of experimental cholera: biologic ativities of purified procholeragen A. J Immunol. 1966 Mar;96(3):440–449. [PubMed] [Google Scholar]
  5. Finkelstein R. A., Yang Z. Rapid test for identification of heat-labile enterotoxin-producing Escherichia coli colonies. J Clin Microbiol. 1983 Jul;18(1):23–28. doi: 10.1128/jcm.18.1.23-28.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Geary S. J., Marchlewicz B. A., Finkelstein R. A. Comparison of heat-labile enterotoxins from porcine and human strains of Escherichia coli. Infect Immun. 1982 Apr;36(1):215–220. doi: 10.1128/iai.36.1.215-220.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Houston C. W., Koo F. C., Peterson J. W. Characterization of Salmonella toxin released by mitomycin C-treated cells. Infect Immun. 1981 May;32(2):916–926. doi: 10.1128/iai.32.2.916-926.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jiwa S. F. Probing for enterotoxigenicity among the salmonellae: an evaluation of biological assays. J Clin Microbiol. 1981 Nov;14(5):463–472. doi: 10.1128/jcm.14.5.463-472.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaura Y. K., Sharma V. K. Production, partial purification and characterization of Salmonella heat-labile enterotoxin. Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Nov;256(1):87–102. [PubMed] [Google Scholar]
  10. Koupal L. R., Deibel R. H. Assay, characterization, and localization of an enterotoxin produced by Salmonella. Infect Immun. 1975 Jan;11(1):14–22. doi: 10.1128/iai.11.1.14-22.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marchlewicz B. A., Finkelstein R. A. Immunological differences among the cholera/coli family of enterotoxins. Diagn Microbiol Infect Dis. 1983 Jun;1(2):129–138. doi: 10.1016/0732-8893(83)90042-1. [DOI] [PubMed] [Google Scholar]
  12. Mekalanos J. J., Collier R. J., Romig W. R. Simple method for purifying choleragenoid, the natural toxoid of Vibrio cholerae. Infect Immun. 1977 Jun;16(3):789–795. doi: 10.1128/iai.16.3.789-795.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Molina N. C., Peterson J. W. Cholera toxin-like toxin released by Salmonella species in the presence of mitomycin C. Infect Immun. 1980 Oct;30(1):224–230. doi: 10.1128/iai.30.1.224-230.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Peterson J. W., Houston C. W., Koo F. C. Influence of cultural conditions on mitomycin C-mediated bacteriophage induction and release of Salmonella toxin. Infect Immun. 1981 Apr;32(1):232–242. doi: 10.1128/iai.32.1.232-242.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Peterson J. W. Salmonella toxin. Pharmacol Ther. 1980;11(3):719–724. doi: 10.1016/0163-7258(80)90046-7. [DOI] [PubMed] [Google Scholar]
  16. Peterson J. W., Sandefur P. D. Evidence of a role for permeability factors in the pathogenesis of salmonellosis. Am J Clin Nutr. 1979 Jan;32(1):197–209. doi: 10.1093/ajcn/32.1.197. [DOI] [PubMed] [Google Scholar]
  17. Sakazaki R., Tamura K., Nakamura A., Kurata T. Enteropathogenic and enterotoxigenic activities on ligated gut loops in rabbits of Salmonella and some other enterobacteria isolated from human patients with diarrhea. Jpn J Med Sci Biol. 1974 Feb;27(1):45–48. doi: 10.7883/yoken1952.27.45. [DOI] [PubMed] [Google Scholar]
  18. Sandefur P. D., Peterson J. W. Isolation of skin permeability factors from culture filtrates of Salmonella typhimurium. Infect Immun. 1976 Sep;14(3):671–679. doi: 10.1128/iai.14.3.671-679.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sandefur P. D., Peterson J. W. Neutralization of Salmonella toxin-induced elongation of Chinese hamster ovary cells by cholera antitoxin. Infect Immun. 1977 Mar;15(3):988–992. doi: 10.1128/iai.15.3.988-992.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sedlock D. M., Deibel R. H. Detection of Salmonella enterotoxin using rabbit ileal loops. Can J Microbiol. 1978 Mar;24(3):268–273. doi: 10.1139/m78-046. [DOI] [PubMed] [Google Scholar]
  21. Stephen J., Wallis T. S., Starkey W. G., Candy D. C., Osborne M. P., Haddon S. Salmonellosis: in retrospect and prospect. Ciba Found Symp. 1985;112:175–192. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES