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. 1986 Sep;24(3):330–332. doi: 10.1128/jcm.24.3.330-332.1986

Rapid microprocedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species.

G M Carlone, M L Thomas, H S Rumschlag, F O Sottnek
PMCID: PMC268907  PMID: 3489731

Abstract

A rapid microprocedure for isolating detergent (sodium N-lauroyl sarcosinate)-insoluble major outer membrane proteins from Haemophilus species produced results qualitatively identical to those obtained with a commonly used preparative isolation procedure. Proteins isolated by both procedures were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after staining with Coomassie brilliant blue R-250. The time for outer membrane protein isolation was substantially reduced with the rapid procedure, allowing a larger number of membrane preparations to be obtained rapidly for routine analysis.

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Selected References

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