Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Sep;24(3):490–492. doi: 10.1128/jcm.24.3.490-492.1986

Failure of rapid agglutination methods to detect oxacillin-resistant Staphylococcus aureus.

P J Ruane, M A Morgan, D M Citron, M E Mulligan
PMCID: PMC268947  PMID: 3489732

Abstract

Although a latex agglutination test (StaphAurex) and a hemagglutination test (Staphyloslide) correctly identified all strains of Staphylococcus aureus that were susceptible or had intermediate susceptibility to oxacillin, 17 of 73 (23%) and 18 of 73 (25%) strains of oxacillin-resistant S. aureus were not identified by StaphAurex and Staphyloslide, respectively. All strains not detected were resistant to trimethoprim-sulfamethoxazole and rifampin.

Full text

PDF
490

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge K. E., Kogos C., Sanders C. V., Marier R. L. Comparison of rapid identification assays for Staphylococcus aureus. J Clin Microbiol. 1984 May;19(5):703–704. doi: 10.1128/jcm.19.5.703-704.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker C. N., Thornsberry C., Hawkinson R. W. Inoculum standardization in antimicrobial susceptibility testing: evaluation of overnight agar cultures and the Rapid Inoculum Standardization System. J Clin Microbiol. 1983 Mar;17(3):450–457. doi: 10.1128/jcm.17.3.450-457.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baker J. S., Bormann M. A., Boudreau D. H. Evaluation of various rapid agglutination methods for the identification of Staphylococcus aureus. J Clin Microbiol. 1985 May;21(5):726–729. doi: 10.1128/jcm.21.5.726-729.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  5. Berke A., Tilton R. C. Evaluation of rapid coagulase methods for the identification of Staphylococcus aureus. J Clin Microbiol. 1986 May;23(5):916–919. doi: 10.1128/jcm.23.5.916-919.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Essers L., Radebold K. Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test. J Clin Microbiol. 1980 Nov;12(5):641–643. doi: 10.1128/jcm.12.5.641-643.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McDougal L. K., Thornsberry C. The role of beta-lactamase in staphylococcal resistance to penicillinase-resistant penicillins and cephalosporins. J Clin Microbiol. 1986 May;23(5):832–839. doi: 10.1128/jcm.23.5.832-839.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pennell D. R., Rott-Petri J. A., Kurzynski T. A. Evaluation of three commercial agglutination tests for the identification of Staphylococcus aureus. J Clin Microbiol. 1984 Oct;20(4):614–617. doi: 10.1128/jcm.20.4.614-617.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Thornsberry C., McDougal L. K. Successful use of broth microdilution in susceptibility tests for methicillin-resistant (heteroresistant) staphylococci. J Clin Microbiol. 1983 Nov;18(5):1084–1091. doi: 10.1128/jcm.18.5.1084-1091.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Walsh T. J., Hansen S. L., Tatem B. A., Auger F., Standiford H. C. Activity of novobiocin against methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother. 1985 Apr;15(4):435–440. doi: 10.1093/jac/15.4.435. [DOI] [PubMed] [Google Scholar]
  11. Woolfrey B. F., Lally R. T., Ederer M. N. An evaluation of three rapid coagglutination tests: Sero-STAT, Accu-Staph, and Staphyloslide, for differentiating Staphylococcus aureus from other species of staphylococci. Am J Clin Pathol. 1984 Mar;81(3):345–348. doi: 10.1093/ajcp/81.3.345. [DOI] [PubMed] [Google Scholar]
  12. Zarzour J. Y., Belle E. A. Evaluation of three test procedures for identification of Staphylococcus aureus from clinical sources. J Clin Microbiol. 1978 Feb;7(2):133–136. doi: 10.1128/jcm.7.2.133-136.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES