Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Oct;24(4):596–599. doi: 10.1128/jcm.24.4.596-599.1986

Simplified enzyme-linked immunosorbent assay for specific antibodies to respiratory syncytial virus.

L Vaur, H Agut, A Garbarg-Chenon, G Prud'Homme de Saint-Maur, J C Nicolas, F Bricout
PMCID: PMC268978  PMID: 3771748

Abstract

A simplified and reliable enzyme-linked immunosorbent assay (ELISA) was applied to the detection of serum antibodies against respiratory syncytial virus (RSV). RSV-infected cells were fixed and dried on 96-well microtiter plates and kept at 4 degrees C. The titers of reference sera were determined by endpoint dilution. A linear relation was found between the titers and the logarithm of absorbance values of sera diluted to 1:1,000 (r = 0.93, P less than 0.001). Measurement of RSV antibodies was done by using a single serum dilution (1:1,000) in conjunction with a standard curve. A strong correlation was found between complement fixation and ELISA results (r = 0.89, P less than 0.001). In addition, the ELISA method exhibited higher titers and a greater sensitivity than did complement fixation, although the applicability of the assay is limited with positive serum samples of low titer.

Full text

PDF
596

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belshe R. B., Van Voris L. P., Mufson M. A., Buynak E. B., McLean A. A., Hilleman M. A. Comparison of enzyme-linked immunosorbent assay and neutralization techniques for measurement of antibody to respiratory syncytial virus: implications for parenteral immunization with live virus vaccine. Infect Immun. 1982 Jul;37(1):160–165. doi: 10.1128/iai.37.1.160-165.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HAMPARIAN V. V., KETLER A., HILLEMAN M. R., REILLY C. M., McCLELLAND L., CORNFELD D., STOKES J., Jr Studies of acute respiratory illnesses caused by respiratory syncytial virus. 1. Laboratory findings in 109 cases. Proc Soc Exp Biol Med. 1961 Apr;106:717–722. doi: 10.3181/00379727-106-26452. [DOI] [PubMed] [Google Scholar]
  3. Hendry R. M., McIntosh K. Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: development and description. J Clin Microbiol. 1982 Aug;16(2):324–328. doi: 10.1128/jcm.16.2.324-328.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hornsleth A., Friis B., Andersen P., Brenøe E. Detection of respiratory syncytial virus in nasopharyngeal secretions by ELISA: comparison with fluorescent antibody technique. J Med Virol. 1982;10(4):273–281. doi: 10.1002/jmv.1890100407. [DOI] [PubMed] [Google Scholar]
  5. Julkunen I. Serological diagnosis of parainfluenza virus infections by enzyme immunoassay with special emphasis on purity of viral antigens. J Med Virol. 1984;14(2):177–187. doi: 10.1002/jmv.1890140212. [DOI] [PubMed] [Google Scholar]
  6. Kahane S., Goldstein V., Sarov I. Detection of IgG antibodies specific for measles virus by enzyme-linked immunosorbent assay (ELISA). Intervirology. 1979;12(1):39–46. doi: 10.1159/000149067. [DOI] [PubMed] [Google Scholar]
  7. Leinikki P. O., Shekarchi I., Tzan N., Madden D. L., Sever J. L. Evaluation of enzyme-linked immunosorbent assay (ELISA) for mumps virus antibodies. Proc Soc Exp Biol Med. 1979 Mar;160(3):363–367. doi: 10.3181/00379727-160-40451. [DOI] [PubMed] [Google Scholar]
  8. Meurman O., Ruuskanen O., Sarkkinen H., Hänninen P., Halonen P. Immunoglobulin class-specific antibody response in respiratory syncytial virus infection measured by enzyme immunoassay. J Med Virol. 1984;14(1):67–72. doi: 10.1002/jmv.1890140110. [DOI] [PubMed] [Google Scholar]
  9. Nandapalan N., Routledge E., Toms G. L. An enzyme-linked immunosorbent assay (ELISA) for IgG and IgA antibodies to respiratory syncytial virus in low dilutions of secretions of human serum and secretions. J Med Virol. 1984;14(3):285–294. doi: 10.1002/jmv.1890140313. [DOI] [PubMed] [Google Scholar]
  10. Pothier P., Nicolas J. C., de Saint Maur G. P., Ghim S., Kazmierczak A., Bricout F. Monoclonal antibodies against respiratory syncytial virus and their use for rapid detection of virus in nasopharyngeal secretions. J Clin Microbiol. 1985 Feb;21(2):286–287. doi: 10.1128/jcm.21.2.286-287.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rice G. P., Casali P., Oldstone M. B. A new solid-phase enzyme-linked immunosorbent assay for specific antibodies to measles virus. J Infect Dis. 1983 Jun;147(6):1055–1059. doi: 10.1093/infdis/147.6.1055. [DOI] [PubMed] [Google Scholar]
  12. Richardson L. S., Yolken R. H., Belshe R. B., Camargo E., Kim H. W., Chanock R. M. Enzyme-linked immunosorbent assay for measurement of serological response to respiratory syncytial virus infection. Infect Immun. 1978 Jun;20(3):660–664. doi: 10.1128/iai.20.3.660-664.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Steinhoff M. C., Hall C. B., Schnabel K. C. Respiratory syncytial virus serology by a simplified enzyme-linked immunosorbent assay. J Clin Microbiol. 1980 Sep;12(3):447–450. doi: 10.1128/jcm.12.3.447-450.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. van Loon A. M., van der Logt J. T., van der Veen J. Enzyme-linked immunosorbent assay for measurement of antibody against cytomegalovirus and rubella virus in a single serum dilution. J Clin Pathol. 1981 Jun;34(6):665–669. doi: 10.1136/jcp.34.6.665. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES