Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Oct;24(4):654–656. doi: 10.1128/jcm.24.4.654-656.1986

Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus.

A J Shepherd, R Swanepoel, P A Leman, S P Shepherd
PMCID: PMC268994  PMID: 3095367

Abstract

The fluorescence focus assay and the plaque assay in CER cells were compared with mouse inoculation for the isolation and titration of Crimean-Congo hemorrhagic fever virus. The fluorescence focus assay and the plaque assay were of similar sensitivity, but both produced 10- to 100-fold lower titers than did mouse inoculation. For specimens from 26 Crimean-Congo hemorrhagic fever patients in South Africa, virus was isolated from 20 by mouse inoculation and from only 11 by cell culturing. Although cell cultures were less sensitive for the isolation of virus from clinical specimens, they produced diagnostic results much more rapidly.

Full text

PDF
654

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benda R., Plaisner V., Hronovský V. Experiences with the adaptation of crimean hemorrhagic fever virus to the CV-1 monkey cell line. Acta Virol. 1975 Jul;19(4):340–348. [PubMed] [Google Scholar]
  2. Buckley S. M. Cross plaque neutralization tests with cloned crimean hemorrhagic fever-congo (CHF-C) and Hazara viruses. Proc Soc Exp Biol Med. 1974 Jun;146(2):594–600. doi: 10.3181/00379727-146-38154. [DOI] [PubMed] [Google Scholar]
  3. Burney M. I., Ghafoor A., Saleen M., Webb P. A., Casals J. Nosocomial outbreak of viral hemorrhagic fever caused by Crimean Hemorrhagic fever-Congo virus in Pakistan, January 1976. Am J Trop Med Hyg. 1980 Sep;29(5):941–947. doi: 10.4269/ajtmh.1980.29.941. [DOI] [PubMed] [Google Scholar]
  4. Causey O. R., Kemp G. E., Madbouly M. H., David-West T. S. Congo virus from domestic livestock, African hedgehog, and arthropods in Nigeria. Am J Trop Med Hyg. 1970 Sep;19(5):846–850. doi: 10.4269/ajtmh.1970.19.846. [DOI] [PubMed] [Google Scholar]
  5. Clerx J. P., Casals J., Bishop D. H. Structural characteristics of nairoviruses (genus Nairovirus, Bunyaviridae). J Gen Virol. 1981 Jul;55(Pt 1):165–178. doi: 10.1099/0022-1317-55-1-165. [DOI] [PubMed] [Google Scholar]
  6. Donets M. A., Rezapkin G. V., Ivanov A. P., Tkachenko E. A. Immunosorbent assays for diagnosis of Crimean-Congo hemorrhagic fever (CCHF). Am J Trop Med Hyg. 1982 Jan;31(1):156–162. doi: 10.4269/ajtmh.1982.31.156. [DOI] [PubMed] [Google Scholar]
  7. Hoogstraal H. The epidemiology of tick-borne Crimean-Congo hemorrhagic fever in Asia, Europe, and Africa. J Med Entomol. 1979 May 22;15(4):307–417. doi: 10.1093/jmedent/15.4.307. [DOI] [PubMed] [Google Scholar]
  8. McCormick J. B., Sasso D. R., Palmer E. L., Kiley M. P. Morphological identification of the agent of Korean haemorrhagic fever (Hantaan virus)as a member of the Bunyaviridae. Lancet. 1982 Apr 3;1(8275):765–768. doi: 10.1016/s0140-6736(82)91812-8. [DOI] [PubMed] [Google Scholar]
  9. McCown J. M., Brandt W. E., Bancroft W. H., Russell P. K. Dimethyl sulfoxide enhancement of phlebotomus fever virus plaque formation. Am J Trop Med Hyg. 1979 Jul;28(4):733–739. [PubMed] [Google Scholar]
  10. Sartorelli A. C., Fischer D. S., Downs W. G. Use of sarcoma 180/TG to prepare hyperimmune ascitic fluid in the mouse. J Immunol. 1966 Apr;96(4):676–682. [PubMed] [Google Scholar]
  11. Shepherd A. J., Swanepoel R., Shepherd S. P., Leman P. A., Blackburn N. K., Hallett A. F. A nosocomial outbreak of Crimean-Congo haemorrhagic fever at Tygerberg Hospital. Part V. Virological and serological observations. S Afr Med J. 1985 Nov 9;68(10):733–736. [PubMed] [Google Scholar]
  12. Smith A. L., Tignor G. H., Mifune K., Motohashi T. Isolation and assay of rabies serogroup viruses in CER cells. Intervirology. 1977;8(2):92–99. doi: 10.1159/000148883. [DOI] [PubMed] [Google Scholar]
  13. Swanepoel R., Struthers J. K., McGillivray G. M. Reversed passive hemagglutination and inhibition with Rift Valley fever and Crimean-Congo hemorrhagic fever viruses. Am J Trop Med Hyg. 1983 May;32(3):610–617. doi: 10.4269/ajtmh.1983.32.610. [DOI] [PubMed] [Google Scholar]
  14. Swanepoel R., Struthers J. K., Shepherd A. J., McGillivray G. M., Nel M. J., Jupp P. G. Crimean-congo hemorrhagic fever in South Africa. Am J Trop Med Hyg. 1983 Nov;32(6):1407–1415. doi: 10.4269/ajtmh.1983.32.1407. [DOI] [PubMed] [Google Scholar]
  15. Tantawi H. H., Al-Moslih M. I., Al-Janabi N. Y., Al-Bana A. S., Mahmud M. I., Jurji F., Yonan M. S., Al-Ani F., Al-Tikriti S. K. Crimean-Congo haemorrhagic fever virus in Iraq: isolation, identification and electron microscopy. Acta Virol. 1980 Dec;24(6):464–467. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES