Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Oct;24(4):665–668. doi: 10.1128/jcm.24.4.665-668.1986

Rapid determination of neutralizing antibodies to Semliki Forest virus in serum by enzyme immunoassay in cell culture with virus-specific monoclonal antibodies.

F H van Tiel, T Harmsen, M Wagenaar, W A Boere, C A Kraaijeveld, H Snippe
PMCID: PMC268997  PMID: 3533979

Abstract

We describe in this study a rapid enzyme immunoassay for the titration of neutralizing antibodies in serum against Semliki Forest virus. For this assay L cells were added to preincubated virus-antiserum mixtures to form monolayers. Six hours after infection by residual, nonneutralized virus, the monolayers were fixed, and the E2 glycoprotein of Semliki Forest virus on the surface of infected cells was quantified with an E2-specific, peroxidase-labeled monoclonal antibody (UM 5.1). The serum antibody titer was defined arbitrarily as the inverse value of that dilution of serum associated with a 25% inhibition of control absorbance values. These titers of both early and later mouse immune sera were similar to those determined in simultaneously performed 50% plaque reduction tests. The results indicate that the enzyme immunoassay (duration, 9 h) is reliable and compares favorably with the conventional plaque reduction test (duration, 25 h) in rapidity, ease of performance, and objectivity.

Full text

PDF
665

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson L. J., Godfrey E., McIntosh K., Hierholzer J. C. Comparison of a monoclonal antibody with a polyclonal serum in an enzyme-linked immunosorbent assay for detecting adenovirus. J Clin Microbiol. 1983 Sep;18(3):463–468. doi: 10.1128/jcm.18.3.463-468.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson L. J., Hierholzer J. C., Bingham P. G., Stone Y. O. Microneutralization test for respiratory syncytial virus based on an enzyme immunoassay. J Clin Microbiol. 1985 Dec;22(6):1050–1052. doi: 10.1128/jcm.22.6.1050-1052.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boere W. A., Benaissa-Trouw B. J., Harmsen M., Kraaijeveld C. A., Snippe H. Neutralizing and non-neutralizing monoclonal antibodies to the E2 glycoprotein of Semliki Forest virus can protect mice from lethal encephalitis. J Gen Virol. 1983 Jun;64(Pt 6):1405–1408. doi: 10.1099/0022-1317-64-6-1405. [DOI] [PubMed] [Google Scholar]
  4. Boere W. A., Harmsen T., Vinjé J., Benaissa-Trouw B. J., Kraaijeveld C. A., Snippe H. Identification of distinct antigenic determinants on Semliki Forest virus by using monoclonal antibodies with different antiviral activities. J Virol. 1984 Nov;52(2):575–582. doi: 10.1128/jvi.52.2.575-582.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bradish C. J., Allner K., Maber H. B. The virulence of original and derived strains of Semliki forest virus for mice, guinea-pigs and rabbits. J Gen Virol. 1971 Aug;12(2):141–160. doi: 10.1099/0022-1317-12-2-141. [DOI] [PubMed] [Google Scholar]
  6. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dimmock N. J. Mechanisms of neutralization of animal viruses. J Gen Virol. 1984 Jun;65(Pt 6):1015–1022. doi: 10.1099/0022-1317-65-6-1015. [DOI] [PubMed] [Google Scholar]
  8. Henderson B. E., Metselaar D., Kirya G. B., Timms G. L. Investigations into yellow fever virus and other arboviruses in the northern regions of Kenya. Bull World Health Organ. 1970;42(5):787–795. [PMC free article] [PubMed] [Google Scholar]
  9. Jensen M. H. Detection of antibodies against hog cholera virus and bovine viral diarrhea virus in porcine serum. A comparative examination using CF, PLA and NPLA assays. Acta Vet Scand. 1981;22(1):85–98. doi: 10.1186/BF03547210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Karpas A., Gillson W., Bevan P. C., Oates J. K. Lytic infection by British AIDS virus and development of rapid cell test for antiviral antibodies. Lancet. 1985 Sep 28;2(8457):695–697. doi: 10.1016/s0140-6736(85)92934-4. [DOI] [PubMed] [Google Scholar]
  11. Kraaijeveld C. A., Harmsen M., Khader Boutahar-Trouw B. Delayed-type hypersensitivity against Semliki Forest virus in mice. Infect Immun. 1979 Feb;23(2):219–223. doi: 10.1128/iai.23.2.219-223.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  13. Phillips D. J., Galland G. G., Reimer C. B., Kendal A. P. Evaluation of a solid-phase immunoassay with fluorescein isothiocyanate-conjugated heterogeneous or monoclonal antibodies for identification of virus isolates, with influenza virus as a model. J Clin Microbiol. 1982 May;15(5):931–937. doi: 10.1128/jcm.15.5.931-937.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shalit I., McKee P. A., Beauchamp H., Waner J. L. Comparison of polyclonal antiserum versus monoclonal antibodies for the rapid diagnosis of influenza A virus infections by immunofluorescence in clinical specimens. J Clin Microbiol. 1985 Nov;22(5):877–879. doi: 10.1128/jcm.22.5.877-879.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Terpstra C., Bloemraad M., Gielkens A. L. The neutralizing peroxidase-linked assay for detection of antibody against swine fever virus. Vet Microbiol. 1984 Apr;9(2):113–120. doi: 10.1016/0378-1135(84)90026-9. [DOI] [PubMed] [Google Scholar]
  16. Van Tiel F. H., Boere W. A., Harmsen T., Benaissa-Trouw B. J., Kraaijeveld C. A., Snippe H. Enzyme immunoassay of interferon with peroxidase-labelled virus-specific monoclonal antibodies. J Gen Virol. 1985 Jun;66(Pt 6):1353–1356. doi: 10.1099/0022-1317-66-6-1353. [DOI] [PubMed] [Google Scholar]
  17. Van Tiel F. H., Harmsen T., Boere W. A., Kraaijeveld C. A., Snippe H. Rapid detection of Semliki Forest virus replication in cell culture with peroxidase-labeled monoclonal antibodies: determination of inhibitory concentrations of monoclonal antibodies and ribavirin. Antiviral Res. 1985;Suppl 1:101–106. doi: 10.1016/s0166-3542(85)80015-2. [DOI] [PubMed] [Google Scholar]
  18. van Tiel F. H., Boere W. A., Harmsen T., Kraaijeveld C. A., Snippe H. Determination of inhibitory concentrations of antiviral agents in cell culture by use of an enzyme immunoassay with virus-specific, peroxidase-labeled monoclonal antibodies. Antimicrob Agents Chemother. 1985 May;27(5):802–805. doi: 10.1128/aac.27.5.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van Tiel F. H., Boere W. A., Vinjé J., Harmsen T., Benaissa-Trouw B. J., Kraaijeveld C. A., Snippe H. Detection of Semliki Forest virus in cell culture by use of an enzyme immunoassay with peroxidase-labeled monoclonal antibodies specific for glycoproteins E1 and E2. J Clin Microbiol. 1984 Sep;20(3):387–390. doi: 10.1128/jcm.20.3.387-390.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES