Abstract
A counterimmunoelectrophoresis (CIE) test was applied to serotype 35 isolates of type D Pasteurella multocida recovered from 32 cases of atrophic rhinitis (in swine) and 3 cases of snuffles (in rabbits). The CIE test was compared with the indirect hemagglutination (IHA) and acriflavine (AF) tests. Results of the CIE test correlated 100% with those of the IHA test whereas results of the AF test correlated 91.43% with those of the IHA test. The CIE test was rapid and simpler to perform compared with the IHA test and more sensitive than the AF test. Cross-reactions were not encountered with capsular antigens of P. multocida types A, B, and E in the CIE test. The CIE test was not found to be suitable for typing type A P. multocida strains.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brogden K. A., Packer R. A. Comparison of Pasteurella multocida serotyping systems. Am J Vet Res. 1979 Sep;40(9):1332–1335. [PubMed] [Google Scholar]
- CARTER G. R. Studies on Pasteurella multocida. I. A hemagglutination test for the identification of serological types. Am J Vet Res. 1955 Jul;16(60):481–484. [PubMed] [Google Scholar]
- Carter G. R., Chengappa M. M. Identification of types B and E Pasteurella multocida by counterimmunoelectrophoresis. Vet Rec. 1981 Feb 14;108(7):145–146. doi: 10.1136/vr.108.7.145. [DOI] [PubMed] [Google Scholar]
- Carter G. R., Rundell S. W. Identification of type A strains of P multocida using staphylococcal hyaluronidase. Vet Rec. 1975 Apr 12;96(15):343–343. doi: 10.1136/vr.96.15.343. [DOI] [PubMed] [Google Scholar]
- Carter G. R., Subronto P. Identification of type D strains of Pasteurella multocida with acriflavine. Am J Vet Res. 1973 Feb;34(2):293–294. [PubMed] [Google Scholar]
- Chengappa M. M., Carter G. R., Bailie W. E. Comparison of indirect hemagglutination and rapid plate agglutination tests with counterimmunoelectrophoresis for typing Pasteurella haemolytica. J Clin Microbiol. 1984 Oct;20(4):660–663. doi: 10.1128/jcm.20.4.660-663.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ingram D. L., Anderson P., Smith D. H. Countercurrent immunoelectrophoresis in the diagnosis of systemic diseases caused by Hemophilus infleunzae type b. J Pediatr. 1972 Dec;81(6):1156–1159. doi: 10.1016/s0022-3476(72)80252-x. [DOI] [PubMed] [Google Scholar]
- Rimler R. B. Coagglutination test for identification of Pasteurella multocida associated with hemorrhagic septicemia. J Clin Microbiol. 1978 Aug;8(2):214–218. doi: 10.1128/jcm.8.2.214-218.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sawada T., Rimler R. B., Rhoades K. R. Indirect hemagglutination test that uses glutaraldehyde-fixed sheep erythrocytes sensitized with extract antigens for detection of Pasteurella antibody. J Clin Microbiol. 1982 May;15(5):752–756. doi: 10.1128/jcm.15.5.752-756.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shackelford P. G., Campbell J., Feigin R. D. Countercurrent immunoelectrophoresis in the evaluation of childhood infections. J Pediatr. 1974 Oct;85(4):478–481. doi: 10.1016/s0022-3476(74)80448-8. [DOI] [PubMed] [Google Scholar]