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. 1986 Nov;24(5):870–872. doi: 10.1128/jcm.24.5.870-872.1986

Detection of cytomegalovirus antibody by enzyme immunoassay and lack of evidence for an effect resulting from strain heterogeneity.

S P Adler, M McVoy
PMCID: PMC269049  PMID: 3021815

Abstract

A total of 259 serum samples from random blood donors were assayed by an enzyme immunoassay for antibody to cytomegalovirus (CMV) with antigens prepared from eight genetically unrelated clinical isolates of CMV and the laboratory-adapted strains AD169 and Towne. A total of 144 serum samples were reactive against all 10 strains, and 115 serum samples lacked immunoglobulin against all 10 strains. The results support the continued use of the AD169 strain of human CMV as the reference antigen for CMV serologic testing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler S. P., Baggett J., Wilson M., Lawrence L., McVoy M. Molecular epidemiology of cytomegalovirus in a nursery: lack of evidence for nosocomial transmission. J Pediatr. 1986 Jan;108(1):117–123. doi: 10.1016/s0022-3476(86)80785-5. [DOI] [PubMed] [Google Scholar]
  2. Adler S. P., Lawrence L. T., Baggett J., Biro V., Sharp D. E. Prevention of transfusion-associated cytomegalovirus infection in very low-birthweight infants using frozen blood and donors seronegative for cytomegalovirus. Transfusion. 1984 Jul-Aug;24(4):333–335. doi: 10.1046/j.1537-2995.1984.24484275576.x. [DOI] [PubMed] [Google Scholar]
  3. Adler S. P., McVoy M., Biro V. G., Britt W. J., Hider P., Marshall D. Detection of cytomegalovirus antibody with latex agglutination. J Clin Microbiol. 1985 Jul;22(1):68–70. doi: 10.1128/jcm.22.1.68-70.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Adler S. P. Molecular epidemiology of cytomegalovirus: evidence for viral transmission to parents from children infected at a day care center. Pediatr Infect Dis. 1986 May-Jun;5(3):315–318. [PubMed] [Google Scholar]
  5. Adler S. P. The molecular epidemiology of cytomegalovirus transmission among children attending a day care center. J Infect Dis. 1985 Oct;152(4):760–768. doi: 10.1093/infdis/152.4.760. [DOI] [PubMed] [Google Scholar]
  6. Adler S. P. Transfusion-associated cytomegalovirus infections. Rev Infect Dis. 1983 Nov-Dec;5(6):977–993. doi: 10.1093/clinids/5.6.977. [DOI] [PubMed] [Google Scholar]
  7. Faix R. G. Cytomegalovirus antigenic heterogeneity can cause false-negative results in indirect hemagglutination and complement fixation antibody assays. J Clin Microbiol. 1985 Nov;22(5):768–771. doi: 10.1128/jcm.22.5.768-771.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fleisher G. R., Starr S. E., Friedman H. M., Plotkin S. A. Vaccination of pediatric nurses with live attenuated cytomegalovirus. Am J Dis Child. 1982 Apr;136(4):294–296. doi: 10.1001/archpedi.1982.03970400012003. [DOI] [PubMed] [Google Scholar]
  9. Plotkin S. A., Smiley M. L., Friedman H. M., Starr S. E., Fleisher G. R., Wlodaver C., Dafoe D. C., Friedman A. D., Grossman R. A., Barker C. F. Towne-vaccine-induced prevention of cytomegalovirus disease after renal transplants. Lancet. 1984 Mar 10;1(8376):528–530. doi: 10.1016/s0140-6736(84)90930-9. [DOI] [PubMed] [Google Scholar]
  10. Waner J. L., Weller T. H., Kevy S. V. Patterns of cytomegaloviral complement-fixing antibody activity: a longitudinal study of blood donors. J Infect Dis. 1973 May;127(5):538–543. doi: 10.1093/infdis/127.5.538. [DOI] [PubMed] [Google Scholar]
  11. Yeager A. S., Grumet F. C., Hafleigh E. B., Arvin A. M., Bradley J. S., Prober C. G. Prevention of transfusion-acquired cytomegalovirus infections in newborn infants. J Pediatr. 1981 Feb;98(2):281–287. doi: 10.1016/s0022-3476(81)80662-2. [DOI] [PubMed] [Google Scholar]
  12. Yeager A. S., Palumbo P. E., Malachowski N., Ariagno R. L., Stevenson D. K. Sequelae of maternally derived cytomegalovirus infections in premature infants. J Pediatr. 1983 Jun;102(6):918–922. doi: 10.1016/s0022-3476(83)80025-0. [DOI] [PubMed] [Google Scholar]
  13. Zablotney S. L., Wentworth B. B., Alexander E. R. Antigenic relatedness of 17 strains of human cytomegalovirus. Am J Epidemiol. 1978 Apr;107(4):336–343. doi: 10.1093/oxfordjournals.aje.a112549. [DOI] [PubMed] [Google Scholar]

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