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. Author manuscript; available in PMC: 2010 Jun 12.
Published in final edited form as: Vaccine. 2009 May 9;27(29):3811–3820. doi: 10.1016/j.vaccine.2009.04.054

Fig. 3.

Fig. 3

IFN-γ production by T cells following vaccination with Fluzone® plus JVRS-100 versus Fluzone® alone. Groups of CD1 mice (n=10) were vaccinated subcutaneously with 5.0 μg of Fluzone® combined with 20.0 ug of JVRS-100 at day 0 and 14. At day 28, splenocytes were prepared and incubated with Fluzone® or a representative heat-inactivated H1N1 (PR/8/34) virus, H3N2 (HKx31) virus, or influenza B (B/Lee/40) virus. Forty-eight hours after stimulation, the supernatant was collected and analyzed for IFN-γ. The IFN-γ content was significantly higher following Fluzone® plus JVRS-100 vaccination compared to Fluzone alone after stimulation of splenocytes in vitro with Fluzone (P<0.05); HKx31 (P<0.005); and PR/8/34 (P<0.005). The response was also higher, but not statistically significant when splenocytes were restimulated with B/Lee/40. P values were calculated using an unpaired, two-tailed Student’s t-test.