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. 1986 Dec;24(6):1089–1092. doi: 10.1128/jcm.24.6.1089-1092.1986

Comparison of Rapid NFT system and conventional methods for identification of nonsaccharolytic gram-negative bacteria.

R Martin, F Siavoshi, D L McDougal
PMCID: PMC269104  PMID: 3536999

Abstract

This study examined the Rapid NFT system (Analytab Products, Plainview, N.Y.) to determine its ability to accurately identify 229 clinical isolates of mostly nonsaccharolytic gram-negative rods. Identifications were classified by the following scheme: correct (corresponding to excellent, very good, good, or acceptable identification as listed in the code book); low discrimination (correct identification among a range of listed possibilities, with additional tests necessary for accurate identification); incorrect. Correct identification was considered correct to species and subspecies for all organisms except Alcaligenes faecalis and "Alcaligenes odorans"; "A. faecalis/odorans" was considered a correct response. By using these criteria, 71.6% of the strains were correctly identified, 17.9% were identified with low discrimination, and 10.5% were incorrectly identified. When consideration was made for incorrect identification resulting from taxonomic problems (e.g., Alcaligenes and Moraxella spp.), incorrect identifications fell to 5.2%. The Rapid NFT system was truly rapid and was easy to use and interpret. Its use of carbon substrate assimilation enables it to provide more accurate identification of medically important nonsaccharolytic bacteria than do other commercially available systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelbaum P. C., Leathers D. J. Evaluation of the rapid NFT system for identification of gram-negative, nonfermenting rods. J Clin Microbiol. 1984 Oct;20(4):730–734. doi: 10.1128/jcm.20.4.730-734.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Appelbaum P. C., Stavitz J., Bentz M. S., von Kuster L. C. Four methods for identification of gram-negative nonfermenting rods: organisms more commonly encountered in clinical specimens. J Clin Microbiol. 1980 Aug;12(2):271–278. doi: 10.1128/jcm.12.2.271-278.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barnishan J., Ayers L. W. Rapid identification of nonfermentative gram-negative rods by the Corning N/F system. J Clin Microbiol. 1979 Feb;9(2):239–243. doi: 10.1128/jcm.9.2.239-243.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bottone E., Allerhand J. Association of mucoid encapsulated Moraxella duplex var. nonliquefaciens with chronic bronchitis. Appl Microbiol. 1968 Feb;16(2):315–319. doi: 10.1128/am.16.2.315-319.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chester V., Cleary T. J. Evaluation of the Minitek system for identification of nonfermentative and nonenteric fermentative Gram-negative bacteria. J Clin Microbiol. 1980 Oct;12(4):509–516. doi: 10.1128/jcm.12.4.509-516.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DuPont H. L., Spink W. W. Infections due to gram-negative organisms: an analysis of 860 patients with bacteremia at the University of Minnesota Medical Center, 1958-1966. Medicine (Baltimore) 1969 Jul;48(4):307–332. doi: 10.1097/00005792-196907000-00003. [DOI] [PubMed] [Google Scholar]
  7. Gardner P., Griffin W. B., Swartz M. N., Kunz L. J. Nonfermentative gram-negative bacilli of nosocomial interest. Am J Med. 1970 Jun;48(6):735–749. doi: 10.1016/s0002-9343(70)80009-2. [DOI] [PubMed] [Google Scholar]
  8. Gilardi G. L. Characterization of nonfermentative nonfastidious gram negative bacteria encountered in medical bacteriology. J Appl Bacteriol. 1971 Sep;34(3):623–644. doi: 10.1111/j.1365-2672.1971.tb02326.x. [DOI] [PubMed] [Google Scholar]
  9. Gilardi G. L. Nonfermentative gram-negative bacteria encountered in clinical specimens. Antonie Van Leeuwenhoek. 1973;39(2):229–242. doi: 10.1007/BF02578855. [DOI] [PubMed] [Google Scholar]
  10. Koestenblatt E. K., Larone D. H., Pavletich K. J. Comparison of the Oxi/Ferm and N/F systems for identification of infrequently encountered nonfermentative and oxidase-positive fermentative bacilli. J Clin Microbiol. 1982 Mar;15(3):384–390. doi: 10.1128/jcm.15.3.384-390.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martin R., Riley P. S., Hollis D. G., Weaver R. E., Krichevsky M. I. Characterization of some groups of gram-negative nonfermentative bacteria by the carbon source alkalinization technique. J Clin Microbiol. 1981 Jul;14(1):39–47. doi: 10.1128/jcm.14.1.39-47.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Oberhofer T. R. Comparison of the API 20E and Oxi/Ferm systems in identification of nonfermentative and oxidase-positive fermentative bacteria. J Clin Microbiol. 1979 Feb;9(2):220–226. doi: 10.1128/jcm.9.2.220-226.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Oberhofer T. R., Rowen J. W. Acetamide agar for differentiation of nonfermentative bacteria. Appl Microbiol. 1974 Oct;28(4):720–721. doi: 10.1128/am.28.4.720-721.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oberhofer T. R., Rowen J. W., Cunningham G. F., Higbee J. W. Evaluation of the oxi/ferm tube system with selected Gram-negative bacteria. J Clin Microbiol. 1977 Dec;6(6):559–566. doi: 10.1128/jcm.6.6.559-566.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rarick H. R., Riley P. S., Martin R. Carbon substrate utilization studies of some cultures of Alcaligenes denitrificans, Alcaligenes faecalis, and Alcaligenes odorans isolated from clinical specimens. J Clin Microbiol. 1978 Sep;8(3):313–319. doi: 10.1128/jcm.8.3.313-319.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sneath P. H., Collins V. G. A study in test reproducibility between laboratories: report of a Pseudomonas Working Party. Antonie Van Leeuwenhoek. 1974;40(4):481–527. doi: 10.1007/BF00403815. [DOI] [PubMed] [Google Scholar]
  17. Snell J. J., Lapage S. P. Carbon source utilization tests as an aid to the classification of non-fermenting gram-negative bacteria. J Gen Microbiol. 1973 Jan;74(1):9–20. doi: 10.1099/00221287-74-1-9. [DOI] [PubMed] [Google Scholar]
  18. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]

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