Abstract
Much nonspecific activity in enzyme immunoassay is due to reactions between anti-bovine antibodies which are commonly present in human serum samples and residual calf serum in viral antigens. Calf serum is used in cultured cells which are used to produce viral antigens. Addition of bovine proteins to serum samples to be tested for viral antibodies reduces this activity.
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Selected References
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- Cowdery J. S., Jr, Steinberg A. D. T cell mediated polyclonal B cell activation induced by cell-bound fetal calf serum. Int Arch Allergy Appl Immunol. 1983;72(2):170–176. doi: 10.1159/000234861. [DOI] [PubMed] [Google Scholar]
- Gray B. M. ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes. J Immunol Methods. 1979;28(1-2):187–192. doi: 10.1016/0022-1759(79)90340-5. [DOI] [PubMed] [Google Scholar]
- Hill H. R., Matsen J. M. Enzyme-linked immunosorbent assay and radioimmunoassay in the serologic diagnosis of infectious diseases. J Infect Dis. 1983 Feb;147(2):258–263. doi: 10.1093/infdis/147.2.258. [DOI] [PubMed] [Google Scholar]
- Murphy M. D., Brunell P. A., Lievens A. W., Shehab Z. M. Effect of early immunization on antibody response to reimmunization with measles vaccine as demonstrated by enzyme-linked immunosorbent assay (ELISA). Pediatrics. 1984 Jul;74(1):90–93. [PubMed] [Google Scholar]
- Shehab Z., Brunell P. A. Enzyme-linked immunosorbent assay for susceptibility to varicella. J Infect Dis. 1983 Sep;148(3):472–476. doi: 10.1093/infdis/148.3.472. [DOI] [PubMed] [Google Scholar]
- Walker W. A. Absorption of protein and protein fragments in the developing intestine: role in immunologic/allergic reactions. Pediatrics. 1985 Jan;75(1 Pt 2):167–171. [PubMed] [Google Scholar]
