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. 1987 Jun;25(6):1009–1013. doi: 10.1128/jcm.25.6.1009-1013.1987

Carumonam enhances reactivity of Escherichia coli with mono- and polyclonal antisera to rough Escherichia coli J5.

B P Overbeek, J F Schellekens, W Lippe, B A Dekker, J Verhoef
PMCID: PMC269125  PMID: 2439534

Abstract

Escherichia coli O111 reacts only slightly with antiserum to its rough mutant E. coli J5 in an enzyme-linked immunosorbent assay. When E. coli O111 was grown in the presence of sub-MICs of the monocyclic beta-lactam antibiotic carumonam, however, the enzyme-linked immunosorbent assay titer increased from 1,280 to 81,920. When the bacteria were grown in the presence of carumonam, the titer that was obtained with antiserum against E. coli O111 was not affected. This reaction was abolished after this antiserum was absorbed with E. coli J5 in the case of the carumonam-treated strain, whereas this absorption did not affect the reaction with E. coli O111. Thus, the O-antigenic side chain of E. coli O111 seems to be affected if this strain is cultured in the presence of carumonam. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a relative loss of the O polysaccharide in E. coli O111 when this strain was grown in the presence of carumonam. Also, a much stronger reaction of the antibiotic-affected lipopolysaccharide with a monoclonal antibody against E. coli J5 lipopolysaccharide was shown in immunoblots. The results of this study indicate that there is a synergism between certain antibiotics and monoclonal antibodies, something that could have clinical implications.

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Selected References

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  1. Baumgartner J. D., Glauser M. P., McCutchan J. A., Ziegler E. J., van Melle G., Klauber M. R., Vogt M., Muehlen E., Luethy R., Chiolero R. Prevention of gram-negative shock and death in surgical patients by antibody to endotoxin core glycolipid. Lancet. 1985 Jul 13;2(8446):59–63. doi: 10.1016/s0140-6736(85)90176-x. [DOI] [PubMed] [Google Scholar]
  2. Benson C. A., Walton F. E., Trenholme G. M. Antibacterial activity of Ro 17-2301 and other antimicrobial agents against cefotaxime-resistant aerobic gram-negative bacilli. Antimicrob Agents Chemother. 1986 Jan;29(1):155–157. doi: 10.1128/aac.29.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Braude A. I., Ziegler E. J., Douglas H., McCutchan J. A. Antibody to cell wall glycolipid of Gram-negative bacteria: induction of immunity to bacteremia and endotoxemia. J Infect Dis. 1977 Aug;136 (Suppl):S167–S173. doi: 10.1093/infdis/136.supplement.s167. [DOI] [PubMed] [Google Scholar]
  4. Bremner D. A. In-vitro activity of the monobactam Ro 17-2301 against clinical isolates of Enterobacteriaceae and Pseudomonas aeruginosa. J Antimicrob Chemother. 1985 Oct;16(4):457–461. doi: 10.1093/jac/16.4.457. [DOI] [PubMed] [Google Scholar]
  5. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  6. Fass R. J., Helsel V. L. In vitro activity of carumonam. Antimicrob Agents Chemother. 1985 Dec;28(6):834–836. doi: 10.1128/aac.28.6.834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gigliotti F., Shenep J. L. Failure of monoclonal antibodies to core glycolipid to bind intact smooth strains of Escherichia coli. J Infect Dis. 1985 Jun;151(6):1005–1011. doi: 10.1093/infdis/151.6.1005. [DOI] [PubMed] [Google Scholar]
  8. Greisman S. E., DuBuy J. B., Woodward C. L. Experimental gram-negative bacterial sepsis: reevaluation of the ability of rough mutant antisera to protect mice. Proc Soc Exp Biol Med. 1978 Jul;158(3):482–490. doi: 10.3181/00379727-158-40231. [DOI] [PubMed] [Google Scholar]
  9. Imada A., Kondo M., Okonogi K., Yukishige K., Kuno M. In vitro and in vivo antibacterial activities of carumonam (AMA-1080), a new N-sulfonated monocyclic beta-lactam antibiotic. Antimicrob Agents Chemother. 1985 May;27(5):821–827. doi: 10.1128/aac.27.5.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johns M. A., Bruins S. C., McCabe W. R. Immunization with R mutants of Salmonella minnesota. II. Serological response to lipid A and the lipopolysaccharide of Re mutants. Infect Immun. 1977 Jul;17(1):9–15. doi: 10.1128/iai.17.1.9-15.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  12. Nelles M. J., Niswander C. A. Mouse monoclonal antibodies reactive with J5 lipopolysaccharide exhibit extensive serological cross-reactivity with a variety of gram-negative bacteria. Infect Immun. 1984 Dec;46(3):677–681. doi: 10.1128/iai.46.3.677-681.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Peter G., Chernow M., Keating M. H., Ryff J. C., Zinner S. H. Limited protective effect of rough mutant antisera in murine Escherichia coli bacteremia. Infection. 1982;10(4):228–232. doi: 10.1007/BF01666916. [DOI] [PubMed] [Google Scholar]
  14. Roy C., Segura C., Reig R., Teixell M., Hermida M., Teruel D., Foz A. Activity of carumonam and aztreonam against Klebsiella species. Eur J Clin Microbiol. 1985 Oct;4(5):519–521. doi: 10.1007/BF02014443. [DOI] [PubMed] [Google Scholar]
  15. Shulman M., Wilde C. D., Köhler G. A better cell line for making hybridomas secreting specific antibodies. Nature. 1978 Nov 16;276(5685):269–270. doi: 10.1038/276269a0. [DOI] [PubMed] [Google Scholar]
  16. Siber G. R., Kania S. A., Warren H. S. Cross-reactivity of rabbit antibodies to lipopolysaccharides of Escherichia coli J5 and other gram-negative bacteria. J Infect Dis. 1985 Nov;152(5):954–964. doi: 10.1093/infdis/152.5.954. [DOI] [PubMed] [Google Scholar]
  17. Teng N. N., Kaplan H. S., Hebert J. M., Moore C., Douglas H., Wunderlich A., Braude A. I. Protection against gram-negative bacteremia and endotoxemia with human monoclonal IgM antibodies. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1790–1794. doi: 10.1073/pnas.82.6.1790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Then R. L., Kohl I. Affinity of carumonam for penicillin-binding proteins. Chemotherapy. 1985;31(4):246–254. doi: 10.1159/000238343. [DOI] [PubMed] [Google Scholar]
  19. Washington J. A., 2nd The effects and significance of subminimal inhibitory concentrations of antibiotics. Rev Infect Dis. 1979 Sep-Oct;1(5):781–786. doi: 10.1093/clinids/1.5.781. [DOI] [PubMed] [Google Scholar]
  20. Young L. S., Hoffman K. R., Stevens P. "Core" glycolipid of enterobacteriaceae: immunofluorescent detection of antigen and antibody. Proc Soc Exp Biol Med. 1975 Jun;149(2):389–396. doi: 10.3181/00379727-149-38814. [DOI] [PubMed] [Google Scholar]
  21. Zak O., Kradolfer F. Effects of subminimal inhibitory concentrations of antibiotics in experimental infections. Rev Infect Dis. 1979 Sep-Oct;1(5):862–879. doi: 10.1093/clinids/1.5.862. [DOI] [PubMed] [Google Scholar]
  22. Ziegler E. J., Douglas H., Sherman J. E., Davis C. E., Braude A. I. Treatment of E. coli and klebsiella bacteremia in agranulocytic animals with antiserum to a UDP-gal epimerase-deficient mutant. J Immunol. 1973 Aug;111(2):433–438. [PubMed] [Google Scholar]
  23. Ziegler E. J., McCutchan J. A., Fierer J., Glauser M. P., Sadoff J. C., Douglas H., Braude A. I. Treatment of gram-negative bacteremia and shock with human antiserum to a mutant Escherichia coli. N Engl J Med. 1982 Nov 11;307(20):1225–1230. doi: 10.1056/NEJM198211113072001. [DOI] [PubMed] [Google Scholar]
  24. de Jongh-Leuvenink J., Bouter A. S., Marcelis J. H., Schellekens J., Verhoef J. Cross-reactivity of monoclonal antibodies against lipopolysaccharides of gram-negative bacteria. Eur J Clin Microbiol. 1986 Apr;5(2):148–151. doi: 10.1007/BF02013970. [DOI] [PubMed] [Google Scholar]
  25. de Jongh-Leuvenink J., Vreede R. W., Marcelis J. H., de Vos M., Verhoef J. Detection of antibodies against lipopolysaccharides of Escherichia coli and Salmonella R and S strains by immunoblotting. Infect Immun. 1985 Dec;50(3):716–720. doi: 10.1128/iai.50.3.716-720.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. de StGroth S. F., Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J Immunol Methods. 1980;35(1-2):1–21. doi: 10.1016/0022-1759(80)90146-5. [DOI] [PubMed] [Google Scholar]
  27. van Dijk W. C., Verbrugh H. A., van Erne-van der Tol M. E., Peters R., Verhoef J. Escherichia coli antibodies in opsonisation and protection against infection. J Med Microbiol. 1981 Nov;14(4):381–389. doi: 10.1099/00222615-14-4-381. [DOI] [PubMed] [Google Scholar]

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