Abstract
The Prompt Inoculation System (3M Co.) was compared with the overnight suspension inoculation procedure used with a broth microdilution anaerobic commercial system (Micro-Media Systems) for differences in MIC. MIC results from both suspension methods using six National Committee for Clinical Laboratory Standards-recommended quality control organisms were identical in 18 instances (75%) and within +/- 1 log2 dilution in 96% of the comparisons. Results with 45 anaerobic clinical isolates also compared satisfactorily; 83% of the results were identical and 97% were within +/- 1 log2 dilution. In addition, the direct (Prompt)-inoculated microdilution trays produced better growth and more valid MIC results; 92% of the clinical isolates produced MIC results versus 79% by the overnight suspension procedure.
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Selected References
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- Baker C. N., Thornsberry C., Hawkinson R. W. Inoculum standardization in antimicrobial susceptibility testing: evaluation of overnight agar cultures and the Rapid Inoculum Standardization System. J Clin Microbiol. 1983 Mar;17(3):450–457. doi: 10.1128/jcm.17.3.450-457.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones R. N., Barry A. L., Cotton J. L., Sutter V. L., Swenson J. M. Collaborative evaluation of the micro-media systems anaerobe susceptibility panel: comparisons with reference methods and test reproducibility. J Clin Microbiol. 1982 Aug;16(2):245–249. doi: 10.1128/jcm.16.2.245-249.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mangels J. I., Lindberg L. H. Evaluation of broth microdilution susceptibility results for anaerobic organisms by use of a rapid direct colony inoculum. J Clin Microbiol. 1985 Feb;21(2):269–272. doi: 10.1128/jcm.21.2.269-272.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murray P. R., Niles A. C. Inoculum preparation for anaerobic susceptibility tests. J Clin Microbiol. 1983 Sep;18(3):733–734. doi: 10.1128/jcm.18.3.733-734.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenblatt J. E., Murray P. R., Sonnenwirth A. C., Joyce J. L. Comparison of anaerobic susceptibility results obtained by different methods. Antimicrob Agents Chemother. 1979 Mar;15(3):351–355. doi: 10.1128/aac.15.3.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swenson J. M., Thornsberry C. Preparing inoculum for susceptibility testing of anaerobes. J Clin Microbiol. 1984 Mar;19(3):321–325. doi: 10.1128/jcm.19.3.321-325.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tally F. P., Cuchural G. J., Jr, Jacobus N. V., Gorbach S. L., Aldridge K., Cleary T., Finegold S. M., Hill G., Iannini P., O'Keefe J. P. Nationwide study of the susceptibility of the Bacteroides fragilis group in the United States. Antimicrob Agents Chemother. 1985 Nov;28(5):675–677. doi: 10.1128/aac.28.5.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wicks J. H., Nelson R. L., Krejcarek G. E. Rapid inoculum standardization system: a novel device for standardization of inocula in antimicrobial susceptibility testing. J Clin Microbiol. 1983 Jun;17(6):1114–1119. doi: 10.1128/jcm.17.6.1114-1119.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]