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. 1987 Jul;25(7):1169–1171. doi: 10.1128/jcm.25.7.1169-1171.1987

Usefulness of an enzyme-linked immunosorbent assay for detection of Giardia antigen in feces.

T E Nash, D A Herrington, M M Levine
PMCID: PMC269169  PMID: 3611309

Abstract

The usefulness of a recently developed enzyme-linked immunosorbent assay which detects Giardia lamblia antigen in feces was determined in experimentally infected humans. Giardia antigen was determined in serially collected fecal specimens from humans inoculated with two Giardia isolates, GS/M and Isr. A total of 277 stools from 18 volunteers were tested, 74 from Isr-inoculated volunteers and 203 from GS/M-inoculated volunteers. None of the five Isr-inoculated volunteers became infected, and none of their stools contained Giardia antigen. In contrast, all of the 13 GS/M-inoculated volunteers became infected, and Giardia antigen was present one or more times in the stools of each. Of 203 stools from GS/M-inoculated volunteers, 73 contained Giardia cysts, and 69 of these (94.5%) had detectable antigen. In contrast, 108 of the 203 specimens were positive for Giardia antigen and only 73 had cysts. Most antigen-positive but cyst-negative specimens occurred during treatment, but during patency 71 stools contained antigen and 65 had cysts. The enzyme-linked immunosorbent assay is infections and is easier to perform.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ament M. E., Rubin C. E. Relation of giardiasis to abnormal intestinal structure and function in gastrointestinal immunodeficiency syndromes. Gastroenterology. 1972 Feb;62(2):216–226. [PubMed] [Google Scholar]
  2. Barbour A. G., Nichols C. R., Fukushima T. An outbreak of giardiasis in a group of campers. Am J Trop Med Hyg. 1976 May;25(3):384–389. doi: 10.4269/ajtmh.1976.25.384. [DOI] [PubMed] [Google Scholar]
  3. Beal C. B., Viens P., Grant R. G., Hughes J. M. A new technique for sampling duodenal contents: demonstration of upper small-bowel pathogens. Am J Trop Med Hyg. 1970 Mar;19(2):349–352. doi: 10.4269/ajtmh.1970.19.349. [DOI] [PubMed] [Google Scholar]
  4. Black R. E., Dykes A. C., Sinclair S. P., Wells J. G. Giardiasis in day-care centers: evidence of person-to-person transmission. Pediatrics. 1977 Oct;60(4):486–491. [PubMed] [Google Scholar]
  5. Brodsky R. E., Spencer H. C., Jr, Schultz M. G. Giardiasis in American travelers to the Soviet Union. J Infect Dis. 1974 Sep;130(3):319–323. doi: 10.1093/infdis/130.3.319. [DOI] [PubMed] [Google Scholar]
  6. Craft J. C., Nelson J. D. Diagnosis of giardiasis by counterimmunoelectrophoresis of feces. J Infect Dis. 1982 Apr;145(4):499–504. doi: 10.1093/infdis/145.4.499. [DOI] [PubMed] [Google Scholar]
  7. Green E. L., Miles M. A., Warhurst D. C. Immunodiagnostic detection of Giardia antigen in faeces by a rapid visual enzyme-linked immunosorbent assay. Lancet. 1985 Sep 28;2(8457):691–693. doi: 10.1016/s0140-6736(85)92932-0. [DOI] [PubMed] [Google Scholar]
  8. Kamath K. R., Murugasu R. A comparative study of four methods for detecting Giardia lamblia in children with diarrheal disease and malabsorption. Gastroenterology. 1974 Jan;66(1):16–21. [PubMed] [Google Scholar]
  9. Kean B. H., William D. C., Luminais S. K. Epidemic of amoebiasis and giardiasis in a biased population. Br J Vener Dis. 1979 Oct;55(5):375–378. doi: 10.1136/sti.55.5.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keystone J. S., Krajden S., Warren M. R. Person-to-person transmission of Giardia lamblia in day-care nurseries. Can Med Assoc J. 1978 Aug 12;119(3):241-2, 247-8. [PMC free article] [PubMed] [Google Scholar]
  11. Nash T. E., McCutchan T., Keister D., Dame J. B., Conrad J. D., Gillin F. D. Restriction-endonuclease analysis of DNA from 15 Giardia isolates obtained from humans and animals. J Infect Dis. 1985 Jul;152(1):64–73. doi: 10.1093/infdis/152.1.64. [DOI] [PubMed] [Google Scholar]
  12. Phillips S. C., Mildvan D., William D. C., Gelb A. M., White M. C. Sexual transmission of enteric protozoa and helminths in a venereal-disease-clinic population. N Engl J Med. 1981 Sep 10;305(11):603–606. doi: 10.1056/NEJM198109103051102. [DOI] [PubMed] [Google Scholar]
  13. RENDTORFF R. C. The experimental transmission of human intestinal protozoan parasites. II. Giardia lamblia cysts given in capsules. Am J Hyg. 1954 Mar;59(2):209–220. doi: 10.1093/oxfordjournals.aje.a119634. [DOI] [PubMed] [Google Scholar]
  14. Rosenthal P., Liebman W. M. Comparative study of stool examinations, duodenal aspiration, and pediatric Entero-Test for giardiasis in children. J Pediatr. 1980 Feb;96(2):278–279. doi: 10.1016/s0022-3476(80)80826-2. [DOI] [PubMed] [Google Scholar]
  15. Schmerin M. J., Jones T. C., Klein H. Giardiasis: association with homosexuality. Ann Intern Med. 1978 Jun;88(6):801–803. doi: 10.7326/0003-4819-88-6-801. [DOI] [PubMed] [Google Scholar]
  16. Ungar B. L., Yolken R. H., Nash T. E., Quinn T. C. Enzyme-linked immunosorbent assay for the detection of Giardia lamblia in fecal specimens. J Infect Dis. 1984 Jan;149(1):90–97. doi: 10.1093/infdis/149.1.90. [DOI] [PubMed] [Google Scholar]
  17. Zierdt W. S. A simple device for concentration of parasite eggs, larvae, and protozoa. Am J Clin Pathol. 1978 Jul;70(1):89–93. doi: 10.1093/ajcp/70.1.89. [DOI] [PubMed] [Google Scholar]

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