TABLE 1.

Strains

ade6 allele	Mating type	Description or sequencea	Base pairsb	Motifc	Straind
+	h90			NA	WS121
52	h−	G796Ae		NA	WS3
M375	h90	TGAGGACGTGAGf	133–144	NA	WS135
M26	h90	GGATGACGTGAGf	133–144	CRE	WS136
M26	h+	his7-366			WS322
M26	h+	his7-366 php2Δ∷kanMX6g			WS310
M26	h+	his7-366 php3Δ∷kanMX6g			WS324
M26	h+	php5Δ∷kanMX6g			WS295
469	h−	C1468Tf		NA	WS315
469	h−	php2Δ∷kanMX6			WS313
469	h−	php3Δ∷kanMX6			WS317
469	h−	php5Δ∷kanMX6			WS311
3074	h90	GGATGACGTCAGh	133–144	CRE	WS137
4001	h90	Δ(-162-451)∷kanMX6-ura4+	—	NA	WS129
4002	h90	CCAATCAi	129–134	7-7	WS224
4002	h+	his7-366			WS326
4002	h+	his7-366 php2Δ∷kanMX6			WS328
4002	h+	his7-366 php3Δ∷kanMX6			WS320
4002	h+	his7-366 php5Δ∷kanMX6			WS293
4003	h90	A__CATGACATCAT	148–160	6-6/8-1	WS330
4003	h−				WS182
4005	h90	ACGTAA_T	138–145	7-1	WS149
4006	h90	CGTCATAi	149–155	7-15	WS150
4008	h90	TATTACGTAAT	160–168	6-1 (Con)	WS331
4008	h−				WS184
4009	h90	ATAGCGTCATATACT	152–165	7-15 (Con)	WS332
4009	h−				WS186
4010	h90	GATGACATAA	151–159	6-21 (Con)	WS365
4010	h−				WS188
4071	h90	ATGATGTCACi	152–161	7-2 (Con)	WS237
4072	h90	ACCCCGCACGCA	167–177	7-4 (Con)	WS240
4073	h90	ACGGCCCCCA_CAATTi	126–141	7-31 (Con)	WS241
4094	h90	GGATGTAAGTj	130–139	10-1	WS374
4095	h90	GGTCTGGACCj	130–139	10-2	WS376
4096	h90	GATGACATCAj	130–139	8-1	WS378
4099	h90	TGAACCCCGCACTGAj	129–143	7-4 (Con)	WS382
4100	h90	GCCCCCACAj	132–140	7-31 (Con)	WS384
4101	h90	ACCCCGCACGTAAT	167–179	7-4	WS386
4102	h90	ATGGCCCCCA_CTATTi	126–141	7-31	WS394
4103	h90	TGACCCCGCACGTj	130–142	7-4	WS409
4104	h90	TGGCCCCCACTATj	130–142	7-31	WS410

^aBases are numbered starting at the first nucleotide of the ade6 open reading frame. Bases in regular type indicate wild-type sequence. Boldface type indicates base substitutions. Insertions or deletions are underlined. For any given ade6 allele, the relevant sequence is shown only once. Additional genotypic information for strains containing the same allele of ade6 is also indicated in this column.

^bThe nucleotide positions of the ade6 sequence shown in the third column. Numbering indicates wild-type sequence before insertions or deletions.

^cIndicates the motif (Table 2, Table S2a, and Table S3) on which the allele is based. NA, not applicable. Con, consensus sequence (Figure S1).

^dIn addition to the ade6 mutations shown in the third column, all strains also contain ura4-D18 and leu1-32, except for WS3 (ura4⁺ leu1⁺). All homothallic strains (h⁹⁰) also contain the plasmid pWS35 (Figure 1).

^eM. Fox and G. Smith, personal communication.

^fSzankasi et al. (1988).

^gMercier et al. (2006).

^hSteiner and Smith (2005b).

ⁱComplement strand shown.

^jThese strains also contain a closely linked nonsense mutation, A121T.