Abstract
The susceptibility of the C6/36 clone of Aedes albopictus monolayer cell cultures was determined with 46 prototype viruses passed through three subcultures. Viral growth was confirmed by titration of the passage material in other susceptible host systems. Nineteen viruses demonstrated good growth in C6/36 cells: coxsackievirus group A type 10 and group B types 2, 3, 4, and 5; enterovirus 69; mumps virus; poliovirus types 1 to 3; reovirus types 1 to 3; vaccinia virus; dengue virus type 2; eastern equine encephalomyelitis virus; La Crosse virus; Rocio virus; and St. Louis encephalitis virus. Ten viruses did not adapt to growth in the C6/36 cultures. Seventeen other virus strains displayed only limited growth which was primarily restricted to the initial C6/36 passage or was detected by hemagglutinin reactions without observable cell degeneration. Of the 46 viruses, 33 (72%) were capable of initiating infection with a demonstrable cytopathic effect in the initial C6/36 passage. Hemagglutination or complement fixation titers or both were obtained with dengue virus type 2, eastern equine encephalomyelitis virus, La Crosse virus, mumps virus, reovirus types 1 to 3, and Rocio, St. Louis encephalitis, and vaccinia viruses.
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- Ajello C. A. Evaluation of Aedes albopictus tissue culture for use in association with arbovirus isolation. J Med Virol. 1979;3(4):301–306. doi: 10.1002/jmv.1890030408. [DOI] [PubMed] [Google Scholar]
- CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
- Chappell W. A., Calisher C. H., Toole R. F., Maness K. C., Sasso D. R., Henderson B. E. Comparison of three methods used to isolate dengue virus type 2. Appl Microbiol. 1971 Dec;22(6):1100–1103. doi: 10.1128/am.22.6.1100-1103.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gubler D. J., Kuno G., Sather G. E., Velez M., Oliver A. Mosquito cell cultures and specific monoclonal antibodies in surveillance for dengue viruses. Am J Trop Med Hyg. 1984 Jan;33(1):158–165. doi: 10.4269/ajtmh.1984.33.158. [DOI] [PubMed] [Google Scholar]
- Hierholzer J. C., Suggs M. T., Hall E. C. Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation. Appl Microbiol. 1969 Nov;18(5):824–833. doi: 10.1128/am.18.5.824-833.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Igarashi A., Buei K., Ueba N., Yoshida M., Ito S., Nakamura H., Sasao F., Fukai K. Isolation of viruses from female Culex tritaeniorhynchus in Aedes albopictus cell cultures. Am J Trop Med Hyg. 1981 Mar;30(2):449–460. doi: 10.4269/ajtmh.1981.30.449. [DOI] [PubMed] [Google Scholar]
- Igarashi A. Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J Gen Virol. 1978 Sep;40(3):531–544. doi: 10.1099/0022-1317-40-3-531. [DOI] [PubMed] [Google Scholar]
- Kuno G. Cultivation of mosquito cell lines in serum-free media and their effects on dengue virus replication. In Vitro. 1983 Sep;19(9):707–713. doi: 10.1007/BF02628962. [DOI] [PubMed] [Google Scholar]
- Kuno G., Gubler D. J., Vélez M., Oliver A. Comparative sensitivity of three mosquito cell lines for isolation of dengue viruses. Bull World Health Organ. 1985;63(2):279–286. [PMC free article] [PubMed] [Google Scholar]
- Race M. W., Williams M. C., Agostini C. F. Dengue in the Caribbean: virus isolation in a mosquito (Aedes pseudoscutellaris) cell line. Trans R Soc Trop Med Hyg. 1979;73(1):18–22. doi: 10.1016/0035-9203(79)90122-6. [DOI] [PubMed] [Google Scholar]
- Singh K. R., Paul S. D. Isolation of Dengue viruses in Aedes albopictus cell cultures. Bull World Health Organ. 1969;40(6):982–983. [PMC free article] [PubMed] [Google Scholar]
- Tesh R. B. A method for the isolation and identification of dengue viruses, using mosquito cell cultures. Am J Trop Med Hyg. 1979 Nov;28(6):1053–1059. doi: 10.4269/ajtmh.1979.28.1053. [DOI] [PubMed] [Google Scholar]