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. 1987 Jul;25(7):1239–1243. doi: 10.1128/jcm.25.7.1239-1243.1987

Whole chromosomal DNA probes for rapid identification of Mycobacterium tuberculosis and Mycobacterium avium complex.

M C Roberts, C McMillan, M B Coyle
PMCID: PMC269184  PMID: 3112180

Abstract

Whole chromosomal DNA probes were used to identify clinical isolates of Mycobacterium tuberculosis, Mycobacterium avium complex, and Mycobacterium gordonae. The probe for M. tuberculosis was prepared from Mycobacterium bovis BCG, which has been shown to be closely related to M. tuberculosis. A probe for the M. avium complex was prepared from three strains representing each of the three DNA homology groups in the M. avium complex. The probes were used in dot blot assays to identify clinical isolates of mycobacteria. The dot blot test correctly identified 57 of the 61 (93%) cultures grown on solid media, and 100% of antibiotic-treated broth-grown cells were correctly identified. Identification by dot blot required a maximum of 48 h. When the probes were tested against 63 positive BACTEC (Johnston Laboratories, Inc., Towson, Md.) cultures of clinical specimens, 59% were correctly identified. However, of the 14 BACTEC cultures that had been treated with antibiotics before being lysed, 13 (93%) were correctly identified.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baess I. Deoxyribonucleic acid relatedness among species of slowly-growing mycobacteria. Acta Pathol Microbiol Scand B. 1979 Aug;87(4):221–226. doi: 10.1111/j.1699-0463.1979.tb02430.x. [DOI] [PubMed] [Google Scholar]
  2. Baess I. Deoxyribonucleic acid relationships between different serovars of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum. Acta Pathol Microbiol Immunol Scand B. 1983 Jun;91(3):201–203. doi: 10.1111/j.1699-0463.1983.tb00033.x. [DOI] [PubMed] [Google Scholar]
  3. Berlin O. G., Zakowski P., Bruckner D. A., Clancy M. N., Johnson B. L., Jr Mycobacterium avium: a pathogen of patients with acquired immunodeficiency syndrome. Diagn Microbiol Infect Dis. 1984 Jun;2(3):213–218. doi: 10.1016/0732-8893(84)90033-6. [DOI] [PubMed] [Google Scholar]
  4. Collins D. M., De Lisle G. W. DNA restriction endonuclease analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG. J Gen Microbiol. 1984 Apr;130(4):1019–1021. doi: 10.1099/00221287-130-4-1019. [DOI] [PubMed] [Google Scholar]
  5. Crawford J. T., Cave M. D., Bates J. H. Characterization of plasmids from strains of Mycobacterium avium-intracellulare. Rev Infect Dis. 1981 Sep-Oct;3(5):949–952. doi: 10.1093/clinids/3.5.949. [DOI] [PubMed] [Google Scholar]
  6. Davidson P. T., Khanijo V., Goble M., Moulding T. S. Treatment of disease due to Mycobacterium intracellulare. Rev Infect Dis. 1981 Sep-Oct;3(5):1052–1059. doi: 10.1093/clinids/3.5.1052. [DOI] [PubMed] [Google Scholar]
  7. Good R. C. Opportunistic pathogens in the genus Mycobacterium. Annu Rev Microbiol. 1985;39:347–369. doi: 10.1146/annurev.mi.39.100185.002023. [DOI] [PubMed] [Google Scholar]
  8. Gross W. M., Hawkins J. E. Radiometric selective inhibition tests for differentiation of Mycobacterium tuberculosis, Mycobacterium bovis, and other mycobacteria. J Clin Microbiol. 1985 Apr;21(4):565–568. doi: 10.1128/jcm.21.4.565-568.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Isaac-Renton J. L., Allen E. A., Chao C. W., Grzybowski S., Whittaker E. I., Black W. A. Isolation and geographic distribution of Mycobacterium other than M. tuberculosis in British Columbia, 1972-81. CMAJ. 1985 Sep 15;133(6):573–576. [PMC free article] [PubMed] [Google Scholar]
  10. Kiehn T. E., Edwards F. F., Brannon P., Tsang A. Y., Maio M., Gold J. W., Whimbey E., Wong B., McClatchy J. K., Armstrong D. Infections caused by Mycobacterium avium complex in immunocompromised patients: diagnosis by blood culture and fecal examination, antimicrobial susceptibility tests, and morphological and seroagglutination characteristics. J Clin Microbiol. 1985 Feb;21(2):168–173. doi: 10.1128/jcm.21.2.168-173.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kirihara J. M., Hillier S. L., Coyle M. B. Improved detection times for Mycobacterium avium complex and Mycobacterium tuberculosis with the BACTEC radiometric system. J Clin Microbiol. 1985 Nov;22(5):841–845. doi: 10.1128/jcm.22.5.841-845.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kuritza A. P., Getty C. E., Shaughnessy P., Hesse R., Salyers A. A. DNA probes for identification of clinically important Bacteroides species. J Clin Microbiol. 1986 Feb;23(2):343–349. doi: 10.1128/jcm.23.2.343-349.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Roberts G. D., Goodman N. L., Heifets L., Larsh H. W., Lindner T. H., McClatchy J. K., McGinnis M. R., Siddiqi S. H., Wright P. Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens. J Clin Microbiol. 1983 Sep;18(3):689–696. doi: 10.1128/jcm.18.3.689-696.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roberts M. C., Hillier S. L., Schoenknecht F. D., Holmes K. K. Comparison of gram stain, DNA probe, and culture for the identification of species of Mobiluncus in female genital specimens. J Infect Dis. 1985 Jul;152(1):74–77. doi: 10.1093/infdis/152.1.74. [DOI] [PubMed] [Google Scholar]
  15. Roberts M. C., Hillier S. L., Schoenknecht F. D., Holmes K. K. Nitrocellulose filter blots for species identification of Mobiluncus curtisii and Mobiluncus mulieris. J Clin Microbiol. 1984 Oct;20(4):826–827. doi: 10.1128/jcm.20.4.826-827.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salyers A. A., Lynn S. P., Gardner J. F. Use of randomly cloned DNA fragments for identification of Bacteroides thetaiotaomicron. J Bacteriol. 1983 Apr;154(1):287–293. doi: 10.1128/jb.154.1.287-293.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tsukamura M. A review of the methods of identification and differentiation of mycobacteria. Rev Infect Dis. 1981 Sep-Oct;3(5):841–861. doi: 10.1093/clinids/3.5.841. [DOI] [PubMed] [Google Scholar]
  18. Wayne L. G. The "atypical" mycobacteria: recognition and disease association. Crit Rev Microbiol. 1985;12(3):185–222. doi: 10.3109/10408418509104429. [DOI] [PubMed] [Google Scholar]
  19. du Moulin G. C., Sherman I. H., Hoaglin D. C., Stottmeier K. D. Mycobacterium avium complex, an emerging pathogen in Massachusetts. J Clin Microbiol. 1985 Jul;22(1):9–12. doi: 10.1128/jcm.22.1.9-12.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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