Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Jul;25(7):1320–1322. doi: 10.1128/jcm.25.7.1320-1322.1987

Saliva as a source of feline leukemia virus antigen for diagnosis of disease.

M G Lewis, K A Wright, L J Lafrado, P J Shanker, N E Palumbo, E D Lemoine, R G Olsen
PMCID: PMC269205  PMID: 3038950

Abstract

An enzyme-linked immunosorbent assay (ELISA) for detection of feline leukemia virus (FeLV) p27 in saliva was tested for its accuracy and sensitivity in diagnosing FeLV infections. Saliva and serum samples from 564 clinical cases were tested with a 99.2% specificity. The overall accuracy of the saliva ELISA reactive to the serum ELISA was 97.9%. Experimentally, the ELISA saliva was the least sensitive in diagnosing early FeLV infections. However, the overall accuracy, ease of use, and simplicity of the test support its use as a screening procedure in clinical practice.

Full text

PDF
1320

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Francis D. P., Essex M., Hardy W. D., Jr Excretion of feline leukaemia virus by naturally infected pet cats. Nature. 1977 Sep 15;269(5625):252–254. doi: 10.1038/269252a0. [DOI] [PubMed] [Google Scholar]
  2. Hardy W. D., Jr, Old L. J., Hess P. W., Essex M., Cotter S. Horizontal transmission of feline leukaemia virus. Nature. 1973 Aug 3;244(5414):266–269. doi: 10.1038/244266a0. [DOI] [PubMed] [Google Scholar]
  3. Hawkins E. C., Johnson L., Pedersen N. C., Winston S. Use of tears for diagnosis of feline leukemia virus infection. J Am Vet Med Assoc. 1986 May 1;188(9):1031–1034. [PubMed] [Google Scholar]
  4. Hoover E. A., Mathes L. E., Rojko J. L., Schaller J. P., Olsen R. G. Modifications of the immunofluorescence assay for feline leukemia virus group-specific antigens. Am J Vet Res. 1978 Dec;39(12):1877–1880. [PubMed] [Google Scholar]
  5. Jarrett O., Golder M. C., Stewart M. F. Detection of transient and persistent feline leukaemia virus infections. Vet Rec. 1982 Mar 6;110(10):225–228. doi: 10.1136/vr.110.10.225. [DOI] [PubMed] [Google Scholar]
  6. Lewis M. G., Mathes L. E., Olsen R. G. Protection against feline leukemia by vaccination with a subunit vaccine. Infect Immun. 1981 Dec;34(3):888–894. doi: 10.1128/iai.34.3.888-894.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lutz H., Pedersen N. C., Durbin R., Theilen G. H. Monoclonal antibodies to three epitopic regions of feline leukemia virus p27 and their use in enzyme-linked immunosorbent assay of p27. J Immunol Methods. 1983 Jan 28;56(2):209–220. doi: 10.1016/0022-1759(83)90413-1. [DOI] [PubMed] [Google Scholar]
  8. Lutz H., Pedersen N. C., Theilen G. H. Course of feline leukemia virus infection and its detection by enzyme-linked immunosorbent assay and monoclonal antibodies. Am J Vet Res. 1983 Nov;44(11):2054–2059. [PubMed] [Google Scholar]
  9. Rickard C. G., Post J. E., Noronha F., Barr L. M. A transmissible virus-induced lymphocytic leukemia of the cat. J Natl Cancer Inst. 1969 Jun;42(6):987–1014. [PubMed] [Google Scholar]
  10. Rojko J. L., Hoover E. A., Mathes L. E., Olsen R. G., Schaller J. P. Pathogenesis of experimental feline leukemia virus infection. J Natl Cancer Inst. 1979 Sep;63(3):759–768. doi: 10.1093/jnci/63.3.759. [DOI] [PubMed] [Google Scholar]
  11. Rojko J. L., Olsen R. G. The immunobiology of the feline leukemia virus. Vet Immunol Immunopathol. 1984 May;6(1-2):107–165. doi: 10.1016/0165-2427(84)90050-3. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES