Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Aug;25(8):1376–1379. doi: 10.1128/jcm.25.8.1376-1379.1987

Subclass restriction of human enterovirus antibodies.

E G Torfason, C B Reimer, H L Keyserling
PMCID: PMC269228  PMID: 3040799

Abstract

We studied antibodies to enteroviruses in four groups of serum specimens: those from healthy adults, cord blood specimens, serum specimens known to contain immunoglobulin M (IgM) to coxsackie B (CB) viruses by radioimmunoassay, and serum specimens from children with symptomatic enteroviral infections. Enzyme-linked immunosorbent assays (ELISAs) were developed to detect the IgG class- and subclass (IgG1, IgG2, IgG3, and IgG4)-specific responses to CB3. The CB3 virus ELISA was not type specific. There was very poor correlation between CB3 virus neutralizing titer and IgG anti-CB3 virus ELISA results, indicating that antibodies to heterologous picornaviruses cross-react with CB3 virus in the assay. All serum specimens tested except one were IgG positive for CB3 virus. All 32 cord serum specimens were positive for IgG1 and IgG3. No enterovirus-specific IgG2 or IgG4 was detected in any serum specimen tested. Most serum specimens from the IgM-positive group, healthy adults, and children with enterovirus infections were positive for IgG1 and IgG3. Class and subclass antibody titers remained constant over time. IgG antibodies to enteroviruses appear to be restricted to the IgG1 and IgG3 subclasses. This pattern is similar to results obtained by other investigators evaluating IgG subclass antibodies to protein antigens.

Full text

PDF
1376

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banatvala J. E., Bryant J., Schernthaner G., Borkenstein M., Schober E., Brown D., De Silva L. M., Menser M. A., Silink M. Coxsackie B, mumps, rubella, and cytomegalovirus specific IgM responses in patients with juvenile-onset insulin-dependent diabetes mellitus in Britain, Austria, and Australia. Lancet. 1985 Jun 22;1(8443):1409–1412. doi: 10.1016/s0140-6736(85)91843-4. [DOI] [PubMed] [Google Scholar]
  2. Brown A. M., Dumas M. L., Reimer C. B., Louie R. E., Harmon R. C. Detection of IgG-associated determinants in reduced and alkylated preparations of human IgG3 by monoclonal antibodies. Vox Sang. 1984;47(6):412–420. doi: 10.1111/j.1423-0410.1984.tb03877.x. [DOI] [PubMed] [Google Scholar]
  3. Catty D., Seger R., Drew R., Ströder J., Metze H. IgG-subclass concentrations in cord sera from premature, full term and small-for-dates babies. Eur J Pediatr. 1977 Jun 1;125(2):89–96. doi: 10.1007/BF00489982. [DOI] [PubMed] [Google Scholar]
  4. Dörries R., ter Meulen V. Specificity of IgM antibodies in acute human coxsackievirus B infections, analysed by indirect solid phase enzyme immunoassay and immunoblot technique. J Gen Virol. 1983 Jan;64(Pt 1):159–167. doi: 10.1099/0022-1317-64-1-159. [DOI] [PubMed] [Google Scholar]
  5. Frisk G., Fohlman J., Kobbah M., Ewald U., Tuvemo T., Diderholm H., Friman G. High frequency of Coxsackie-B-virus-specific IgM in children developing type I diabetes during a period of high diabetes morbidity. J Med Virol. 1985 Nov;17(3):219–227. doi: 10.1002/jmv.1890170303. [DOI] [PubMed] [Google Scholar]
  6. Frisk G., Torfason E. G., Diderholm H. Reverse radioimmunoassays of IgM and IgG antibodies to Coxsackie B viruses in patients with acute myopericarditis. J Med Virol. 1984;14(3):191–200. doi: 10.1002/jmv.1890140302. [DOI] [PubMed] [Google Scholar]
  7. King M. L., Shaikh A., Bidwell D., Voller A., Banatvala J. E. Coxsackie-B-virus-specific IgM responses in children with insulin-dependent (juvenile-onset; type I) diabetes mellitus. Lancet. 1983 Jun 25;1(8339):1397–1399. doi: 10.1016/s0140-6736(83)92353-x. [DOI] [PubMed] [Google Scholar]
  8. Linde G. A., Hammarström L., Persson M. A., Smith C. I., Sundqvist V. A., Wahren B. Virus-specific antibody activity of different subclasses of immunoglobulins G and A in cytomegalovirus infections. Infect Immun. 1983 Oct;42(1):237–244. doi: 10.1128/iai.42.1.237-244.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mertens T., Pika U., Eggers H. J. Cross antigenicity among enteroviruses as revealed by immunoblot technique. Virology. 1983 Sep;129(2):431–442. doi: 10.1016/0042-6822(83)90181-2. [DOI] [PubMed] [Google Scholar]
  10. Papadea C., Check I. J., Reimer C. B. Monoclonal antibody-based solid-phase immunoenzymometric assays for quantifying human immunoglobulin G and its subclasses in serum. Clin Chem. 1985 Dec;31(12):1940–1945. [PubMed] [Google Scholar]
  11. Pattison J. R. Tests for coxsackie B virus-specific IgM. J Hyg (Lond) 1983 Jun;90(3):327–332. doi: 10.1017/s0022172400028965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pitcher-Wilmott R. W., Hindocha P., Wood C. B. The placental transfer of IgG subclasses in human pregnancy. Clin Exp Immunol. 1980 Aug;41(2):303–308. [PMC free article] [PubMed] [Google Scholar]
  13. Pugh S. F. Heterotypic reactions in a radioimmunoassay for coxsackie B virus specific IgM. J Clin Pathol. 1984 Apr;37(4):433–439. doi: 10.1136/jcp.37.4.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rautonen N., Pelkonen J., Sipinen S., Käyhty H., Mäkelä O. Isotype concentrations of human antibodies to group A meningococcal polysaccharide. J Immunol. 1986 Oct 15;137(8):2670–2675. [PubMed] [Google Scholar]
  15. Reimer C. B., Phillips D. J., Aloisio C. H., Moore D. D., Galland G. G., Wells T. W., Black C. M., McDougal J. S. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984 Fall;3(3):263–275. doi: 10.1089/hyb.1984.3.263. [DOI] [PubMed] [Google Scholar]
  16. Rubin R. L., Tang F. L., Lucas A. H., Spiegelberg H. L., Tan E. M. IgG subclasses of anti-tetanus toxoid antibodies in adult and newborn normal subjects and in patients with systemic lupus erythematosus, Sjogren's syndrome, and drug-induced autoimmunity. J Immunol. 1986 Oct 15;137(8):2522–2527. [PubMed] [Google Scholar]
  17. Skvaril F., Schilt U. Characterization of the subclasses and light chain types of IgG antibodies to rubella. Clin Exp Immunol. 1984 Mar;55(3):671–676. [PMC free article] [PubMed] [Google Scholar]
  18. Torfason E. G., Frisk G., Diderholm H. Indirect and reverse radioimmunoassays and their apparent specificities in the detection of antibodies to enteroviruses in human sera. J Med Virol. 1984;13(1):13–31. doi: 10.1002/jmv.1890130103. [DOI] [PubMed] [Google Scholar]
  19. Vartdal F., Vandvik B. Multiple sclerosis: subclasses of intrathecally synthesized IgG and measles and varicella zoster virus IgG antibodies. Clin Exp Immunol. 1983 Dec;54(3):641–647. [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES