Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Aug;25(8):1401–1405. doi: 10.1128/jcm.25.8.1401-1405.1987

Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining.

L S Zhao, M L Landry, E S Balkovic, G D Hsiung
PMCID: PMC269234  PMID: 3040800

Abstract

Tremendous interest has been generated in the commercial kits now available that incorporate herpes simplex virus isolation in cell culture with immunoperoxidase staining for viral antigen detection. Most studies comparing commercial kits with conventional cell culture techniques have found the kits to be less sensitive. However, different cell cultures were used for the two methods. In this study, mink lung, rabbit kidney, MRC-5, and Vero cells were compared for reisolation of herpes simplex virus from clinical specimens in which viral infectivity titers were concurrently determined. When specimens contained high titers of infectious virus, the cell system used made little difference and all specimens were detected by immunoperoxidase staining at 48 h postinoculation. However, when specimens contained low concentrations of virus, the differences in sensitivity between cell systems became apparent in rapidity of detection and overall isolation rate. Mink lung and rabbit kidney cells were both more sensitive than MRC-5 cells; Vero cells were significantly less sensitive than the other cells tested. The application of immunoperoxidase staining shortened the time to virus detection and lessened, but did not eliminate, the differences between the cell systems. Cytopathic effects alone in the most sensitive cell system equaled or exceeded immunoperoxidase staining applied in less-sensitive cell cultures.

Full text

PDF
1401

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balkovic E. S., Hsiung G. D. Comparison of immunofluorescence with commercial monoclonal antibodies to biochemical and biological techniques for typing clinical herpes simplex virus isolates. J Clin Microbiol. 1985 Nov;22(5):870–872. doi: 10.1128/jcm.22.5.870-872.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Callihan D. R., Menegus M. A. Rapid detection of herpes simplex virus in clinical specimens with human embryonic lung fibroblast and primary rabbit kidney cell cultures. J Clin Microbiol. 1984 Apr;19(4):563–565. doi: 10.1128/jcm.19.4.563-565.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chang R. S., Arnold D., Chang Y. Y., Pan I. H., Jordan G. W. Relative sensitivity of cell culture systems in the detection of herpes simplex viruses. Diagn Microbiol Infect Dis. 1986 Jul;5(2):135–141. doi: 10.1016/0732-8893(86)90115-x. [DOI] [PubMed] [Google Scholar]
  4. Fayram S. L., Aarnaes S. L., Peterson E. M., de la Maza L. M. Evaluation of five cell types for the isolation of herpes simplex virus. Diagn Microbiol Infect Dis. 1986 Jul;5(2):127–133. doi: 10.1016/0732-8893(86)90114-8. [DOI] [PubMed] [Google Scholar]
  5. Fayram S. L., Aarnaes S., de la Maza L. M. Comparison of cultureset to a conventional tissue culture-fluorescent-antibody technique for isolation and identification of herpes simplex virus. J Clin Microbiol. 1983 Jul;18(1):215–216. doi: 10.1128/jcm.18.1.215-216.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fung J. C., Shanley J., Tilton R. C. Comparison of the detection of herpes simplex virus in direct clinical specimens with herpes simplex virus-specific DNA probes and monoclonal antibodies. J Clin Microbiol. 1985 Nov;22(5):748–753. doi: 10.1128/jcm.22.5.748-753.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gleaves C. A., Wilson D. J., Wold A. D., Smith T. F. Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation. J Clin Microbiol. 1985 Jan;21(1):29–32. doi: 10.1128/jcm.21.1.29-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayden F. G., Sorensen A. S., Bateman J. A. Comparison of the Immulok cultureset kit and virus isolation for detection of herpes simplex virus in clinical specimens. J Clin Microbiol. 1983 Jul;18(1):222–224. doi: 10.1128/jcm.18.1.222-224.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  10. Hughes J. H., Mann D. R., Hamparian V. V. Viral isolation versus immune staining of infected cell cultures for the laboratory diagnosis of herpes simplex virus infections. J Clin Microbiol. 1986 Sep;24(3):487–489. doi: 10.1128/jcm.24.3.487-489.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnson F. B., Leavitt R. W., Richards D. F. Comparison of the Scott Selecticult-HSV kit with conventional culture and direct immunoperoxidase staining for detection of herpes simplex virus in cultures of clinical specimens. J Clin Microbiol. 1985 Mar;21(3):438–441. doi: 10.1128/jcm.21.3.438-441.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Landry M. L., Mayo D. R., Hsiung G. D. Comparison of guinea pig embryo cells, rabbit kidney cells, and human embryonic lung fibroblast cell strains for isolation of herpes simplex virus. J Clin Microbiol. 1982 May;15(5):842–847. doi: 10.1128/jcm.15.5.842-847.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mayo D. R., Brennan T., Egbertson S. H., Moore D. F. Rapid herpes simplex virus detection in clinical samples submitted to a state virology laboratory. J Clin Microbiol. 1985 May;21(5):768–771. doi: 10.1128/jcm.21.5.768-771.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moore D. F. Comparison of human fibroblast cells and primary rabbit kidney cells for isolation of herpes simplex virus. J Clin Microbiol. 1984 Apr;19(4):548–549. doi: 10.1128/jcm.19.4.548-549.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Moseley R. C., Corey L., Benjamin D., Winter C., Remington M. L. Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection. J Clin Microbiol. 1981 May;13(5):913–918. doi: 10.1128/jcm.13.5.913-918.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Phillips L. E., Magliolo R. A., Stehlik M. L., Whiteman P. A., Faro S., Rogers T. E. Retrospective evaluation of the isolation and identification of herpes simplex virus with Cultureset and human fibroblasts. J Clin Microbiol. 1985 Aug;22(2):255–258. doi: 10.1128/jcm.22.2.255-258.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rubin S. J., Rogers S. Comparison of Cultureset and primary rabbit kidney cell culture for the detection of herpes simplex virus. J Clin Microbiol. 1984 Jun;19(6):920–922. doi: 10.1128/jcm.19.6.920-922.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Salmon V. C., Michaels B. K., Turner R. B. Comparison of cell culture with an immunoperoxidase kit for rapid diagnosis of herpes simplex virus infections. Diagn Microbiol Infect Dis. 1984 Sep;2(4):343–345. doi: 10.1016/0732-8893(84)90067-1. [DOI] [PubMed] [Google Scholar]
  19. Salmon V. C., Stanberry L. R., Overall J. C., Jr More rapid isolation of herpes simplex virus in a continuous line of mink lung cells than in Vero or human fibroblast cells. Diagn Microbiol Infect Dis. 1984 Sep;2(4):317–324. doi: 10.1016/0732-8893(84)90063-4. [DOI] [PubMed] [Google Scholar]
  20. Salmon V. C., Turner R. B., Speranza M. J., Overall J. C., Jr Rapid detection of herpes simplex virus in clinical specimens by centrifugation and immunoperoxidase staining. J Clin Microbiol. 1986 Apr;23(4):683–686. doi: 10.1128/jcm.23.4.683-686.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sewell D. L., Horn S. A., Dilbeck P. W. Comparison of Cultureset and Bartels Immunodiagnostics with conventional tissue culture for isolation and identification of herpes simplex virus. J Clin Microbiol. 1984 May;19(5):705–706. doi: 10.1128/jcm.19.5.705-706.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES