Abstract
Continuous cell lines were tested for their potential use in distinguishing pathogenic from nonpathogenic Listeria species. Listeria monocytogenes and Listeria ivanovii strains were lethal for mice, and culture filtrates were cytotoxic for cultured cells and hemolytic for sheep erythrocytes, while Listeria innocua, Listeria grayi, and Listeria murrayi strains were negative in all three tests. The eight cell lines tested were all affected but varied in sensitivity, with the Chinese hamster ovary cell line being the most sensitive. Cytolytic effect was noted within minutes of the addition of undiluted filtrates, with optimum titers obtained by 24 h.
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- Gaillard J. L., Berche P., Sansonetti P. Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infect Immun. 1986 Apr;52(1):50–55. doi: 10.1128/iai.52.1.50-55.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Khan M. A., Seaman T. A., Woodbine M. Listeria monocytogenes-haemolysin: lecithinase. Zentralbl Bakteriol Orig A. 1973 Oct;225(1):66–79. [PubMed] [Google Scholar]
- Kingdon G. C., Sword C. P. Effects of Listeria monocytogenes Hemolysin on Phagocytic Cells and Lysosomes. Infect Immun. 1970 Apr;1(4):356–362. doi: 10.1128/iai.1.4.356-362.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lemeland J. F., Allaire R., Boiron H. Contribution à l'étude de l'hémolysine de "Listeria monocytogenes" (listériolysine) Pathol Biol (Paris) 1974 Nov;22(9):763–770. [PubMed] [Google Scholar]
- MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nieman R. E., Lorber B. Listeriosis in adults: a changing pattern. Report of eight cases and review of the literature, 1968-1978. Rev Infect Dis. 1980 Mar-Apr;2(2):207–227. doi: 10.1093/clinids/2.2.207. [DOI] [PubMed] [Google Scholar]
- Ralovich B., Emödy L., Mérö E. Proceedings: Biological properties of virulent and avirulent Listeria monocytogenes strains. Acta Microbiol Acad Sci Hung. 1972;19(4):323–326. [PubMed] [Google Scholar]
- Raphael R. A., Sattar S. A., Springthorpe V. S. Long-term survival of human rotavirus in raw and treated river water. Can J Microbiol. 1985 Feb;31(2):124–128. doi: 10.1139/m85-024. [DOI] [PubMed] [Google Scholar]
- Seeliger H. P. Proceedings: New outlook on the epidemiology and epizoology of listeriosis. Acta Microbiol Acad Sci Hung. 1972;19(4):273–286. [PubMed] [Google Scholar]
- Siddique I. H. Cytotoxic activity of hemolysin from Listeria monocytogenes onL-M strain of mouse cells. Can J Microbiol. 1969 Aug;15(8):955–957. doi: 10.1139/m69-166. [DOI] [PubMed] [Google Scholar]
- Siddique I. H., Lin I. F., Chung R. A. Purification and characterization of hemolysin produced by Listeria monocytogenes. Am J Vet Res. 1974 Feb;35(2):289–286. [PubMed] [Google Scholar]
- Skalka B., Smola J., Elischerová K. Hemolytic phenomenons under the cultivation of Listeria innocua. Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Feb;253(4):559–565. [PubMed] [Google Scholar]
- Skalka B., Smola J., Elischerová K. Routine test for in vitro differentiation of pathogenic and apathogenic Listeria monocytogenes strains. J Clin Microbiol. 1982 Mar;15(3):503–507. doi: 10.1128/jcm.15.3.503-507.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swierenga S. H., Whitfield J. F., Karasaki S. Loss of proliferative calcium dependence: simple in vitro indicator of tumorigenicity. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6069–6072. doi: 10.1073/pnas.75.12.6069. [DOI] [PMC free article] [PubMed] [Google Scholar]