Abstract
Specific DNA probes (Gen-Probe Corp., San Diego, Calif.) for Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium tuberculosis were compared with conventional methods for the identification of isolates of the Mycobacterium avium complex. A total of 56 isolates of M. avium complex were recovered from 34 respiratory, 13 blood, 6 stool, and 3 urine samples from 23 patients. A total of 33 isolates were tested directly from Middlebrook 7H11 agar plates, and 23 isolates were tested directly from BACTEC radiometric 12B bottles (Johnston Laboratories, Inc., Towson, Md.). Of the 56 M. avium complex isolates, 41 tested positive with the M. avium probe, 4 were positive with the M. intracellulare probe, and 7 were positive with both probes. Four direct tests from BACTEC bottles were initially negative but were subsequently M. avium probe positive when subcultures from Lowenstein-Jensen agar were tested. All 56 strains were negative when tested with the M. tuberculosis probe.
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- Armstrong D., Gold J. W., Dryjanski J., Whimbey E., Polsky B., Hawkins C., Brown A. E., Bernard E., Kiehn T. E. Treatment of infections in patients with the acquired immunodeficiency syndrome. Ann Intern Med. 1985 Nov;103(5):738–743. doi: 10.7326/0003-4819-103-5-738. [DOI] [PubMed] [Google Scholar]
- Baess I. Deoxyribonucleic acid relatedness among species of slowly-growing mycobacteria. Acta Pathol Microbiol Scand B. 1979 Aug;87(4):221–226. doi: 10.1111/j.1699-0463.1979.tb02430.x. [DOI] [PubMed] [Google Scholar]
- Baess I. Deoxyribonucleic acid relationships between different serovars of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum. Acta Pathol Microbiol Immunol Scand B. 1983 Jun;91(3):201–203. doi: 10.1111/j.1699-0463.1983.tb00033.x. [DOI] [PubMed] [Google Scholar]
- Kiehn T. E., Cammarata R. Laboratory diagnosis of mycobacterial infections in patients with acquired immunodeficiency syndrome. J Clin Microbiol. 1986 Nov;24(5):708–711. doi: 10.1128/jcm.24.5.708-711.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiehn T. E., Edwards F. F., Brannon P., Tsang A. Y., Maio M., Gold J. W., Whimbey E., Wong B., McClatchy J. K., Armstrong D. Infections caused by Mycobacterium avium complex in immunocompromised patients: diagnosis by blood culture and fecal examination, antimicrobial susceptibility tests, and morphological and seroagglutination characteristics. J Clin Microbiol. 1985 Feb;21(2):168–173. doi: 10.1128/jcm.21.2.168-173.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lévy-Frébault V., Pangon B., Buré A., Katlama C., Marche C., David H. L. Mycobacterium simiae and Mycobacterium avium-M. intracellulare mixed infection in acquired immune deficiency syndrome. J Clin Microbiol. 1987 Jan;25(1):154–157. doi: 10.1128/jcm.25.1.154-157.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Males B. M., West T. E., Bartholomew W. R. Mycobacterium haemophilum infection in a patient with acquired immune deficiency syndrome. J Clin Microbiol. 1987 Jan;25(1):186–190. doi: 10.1128/jcm.25.1.186-190.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schaefer W. B. Serologic identification and classification of the atypical mycobacteria by their agglutination. Am Rev Respir Dis. 1965 Dec;92(6):85–93. doi: 10.1164/arrd.1965.92.6P2.85. [DOI] [PubMed] [Google Scholar]
- Sherer R., Sable R., Sonnenberg M., Cooper S., Spencer P., Schwimmer S., Kocka F., Muthuswamy P., Kallick C. Disseminated infection with Mycobacterium kansasii in the acquired immunodeficiency syndrome. Ann Intern Med. 1986 Nov;105(5):710–712. doi: 10.7326/0003-4819-105-5-710. [DOI] [PubMed] [Google Scholar]