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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Biochemistry. 2008 Jan 18;47(9):2968–2977. doi: 10.1021/bi701923h

Fig. 7. Fluorescence spectroscopy of apoE3 and apoE4 single Trp mutants.

Fig. 7

Solutions of the proteins (100 µg/ml) were excited at 295 nm to obtain the emission fluorescence from W264. (A) Fluorescence spectra for apoE3 W@264 (a) and apoE4 W@264 (b). (B) Stern-Volmer plot comparing quenching by KI of W264 fluorescence from apoE3 (○) and apoE4 (●). The data for N-acetyltryptophanamide are also shown for comparison (△). The inset shows a modified Stern-Volmer plot. The results from 2–4 independent experiments, each done in triplicate, are shown.