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. 1987 Sep;25(9):1611–1622. doi: 10.1128/jcm.25.9.1611-1622.1987

High-frequency switching in Candida strains isolated from vaginitis patients.

D R Soll 1, C J Langtimm 1, J McDowell 1, J Hicks 1, R Galask 1
PMCID: PMC269293  PMID: 2821060

Abstract

High-frequency switching and strain variability at the site of infection was assessed in 11 patients with acute Candida albicans vaginitis. By cloning cells directly from the site of infection, it was demonstrated that 4 of the 11 isolates contained multiple-switch phenotypes at the site of infection and that 9 of the 11 isolates were in a high-frequency mode of switching (10(-2) to 10(-3)). Isolates could be separated into four general categories of switching repertoires. To demonstrate that multiple phenotypes at the site of a single infection represented the same strain, EcoRI digests of total cell DNA were separated on agarose gels, and Southern hybridization patterns with two cloned midrepeat sequences were compared.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bedell G. W., Soll D. R. Effects of low concentrations of zinc on the growth and dimorphism of Candida albicans: evidence for zinc-resistant and -sensitive pathways for mycelium formation. Infect Immun. 1979 Oct;26(1):348–354. doi: 10.1128/iai.26.1.348-354.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chandler F. W. Pathology of the mycoses in patients with the acquired immunodeficiency syndrome (AIDS). Curr Top Med Mycol. 1985;1:1–23. doi: 10.1007/978-1-4613-9547-8_1. [DOI] [PubMed] [Google Scholar]
  3. Cryer D. R., Eccleshall R., Marmur J. Isolation of yeast DNA. Methods Cell Biol. 1975;12:39–44. doi: 10.1016/s0091-679x(08)60950-4. [DOI] [PubMed] [Google Scholar]
  4. DeGregorio M. W., Lee W. M., Linker C. A., Jacobs R. A., Ries C. A. Fungal infections in patients with acute leukemia. Am J Med. 1982 Oct;73(4):543–548. doi: 10.1016/0002-9343(82)90334-5. [DOI] [PubMed] [Google Scholar]
  5. Knight L., Fletcher J. Growth of Candida albicans in saliva: stimulation by glucose associated with antibiotics, corticosteroids, and diabetes mellitus. J Infect Dis. 1971 Apr;123(4):371–377. doi: 10.1093/infdis/123.4.371. [DOI] [PubMed] [Google Scholar]
  6. Lee K. L., Buckley H. R., Campbell C. C. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans. Sabouraudia. 1975 Jul;13(2):148–153. doi: 10.1080/00362177585190271. [DOI] [PubMed] [Google Scholar]
  7. Masterton G., Henderson J. N., Napier I., Moffett M. Letter: Vaginal candidosis. Br Med J. 1976 Mar 20;1(6011):712–713. doi: 10.1136/bmj.1.6011.712-d. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Myerowitz R. L., Pazin G. J., Allen C. M. Disseminated candidiasis. Changes in incidence, underlying diseases, and pathology. Am J Clin Pathol. 1977 Jul;68(1):29–38. doi: 10.1093/ajcp/68.1.29. [DOI] [PubMed] [Google Scholar]
  9. Parker J. C., Jr, McCloskey J. J., Knauer K. A. Pathobiologic features of human candidiasis. A common deep mycosis of the brain, heart and kidney in the altered host. Am J Clin Pathol. 1976 Jun;65(6):991–1000. doi: 10.1093/ajcp/65.6.991. [DOI] [PubMed] [Google Scholar]
  10. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  11. Slutsky B., Buffo J., Soll D. R. High-frequency switching of colony morphology in Candida albicans. Science. 1985 Nov 8;230(4726):666–669. doi: 10.1126/science.3901258. [DOI] [PubMed] [Google Scholar]
  12. Slutsky B., Staebell M., Anderson J., Risen L., Pfaller M., Soll D. R. "White-opaque transition": a second high-frequency switching system in Candida albicans. J Bacteriol. 1987 Jan;169(1):189–197. doi: 10.1128/jb.169.1.189-197.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sobel J. D. Epidemiology and pathogenesis of recurrent vulvovaginal candidiasis. Am J Obstet Gynecol. 1985 Aug 1;152(7 Pt 2):924–935. doi: 10.1016/s0002-9378(85)80003-x. [DOI] [PubMed] [Google Scholar]
  14. Sobel J. D. Vulvovaginal candidiasis--what we do and do not know. Ann Intern Med. 1984 Sep;101(3):390–392. doi: 10.7326/0003-4819-101-3-390. [DOI] [PubMed] [Google Scholar]
  15. Soll D. R., Bedell G. W., Brummel M. Zinc and regulation of growth and phenotype in the infectious yeast Candida albicans. Infect Immun. 1981 Jun;32(3):1139–1147. doi: 10.1128/iai.32.3.1139-1147.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tapper-Jones L. M., Aldred M. J., Walker D. M., Hayes T. M. Candidal infections and populations of Candida albicans in mouths of diabetics. J Clin Pathol. 1981 Jul;34(7):706–711. doi: 10.1136/jcp.34.7.706. [DOI] [PMC free article] [PubMed] [Google Scholar]

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