Table 2.

Ba ENR inhibition and antibacterial activity for modification of ring B.

Compound	ortho	meta	para	IC50 (μM) or %inhibition at 1μM	MIC[b] (μg/mL)
Triclosan	Cl	---	Cl	0.6 ± 0.0	3.1
55	---	---	---	0.5 ± 0.1	32
3	---	---	OH	2.6%	>64
20	---	---	NH2	7.1 ± 1.2	12
18	---	---		1.6%	111
19		---	---	0%	111
22	---	---		>12[a]	4.9
4	---	---	NO2	7.2%	3.3
5	NO2	---	---	7.7 ± 0.7	13.3
6	Cl	---	NO2	0.3 ± 0.0	<0.1 – 3.1
7	---	---	Ph	>6.25	1.9
32	---	---		15.5%	>145
33	---		---	9.9%	2.3

^[a]Saturation with inhibitor was not obtained over the concentration range tested. The percent inhibition of BaENR showed a linear response to increasing inhibitor concentrations.

^[b]MIC values are against ΔANR B. anthracis.