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. 1987 Oct;25(10):1822–1829. doi: 10.1128/jcm.25.10.1822-1829.1987

Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization.

J R Ticehurst 1, S M Feinstone 1, T Chestnut 1, N C Tassopoulos 1, H Popper 1, R H Purcell 1
PMCID: PMC269349  PMID: 2822759

Abstract

Hepatitis A virus (HAV) RNA was extracted from cell culture, serum, liver, and feces and then detected by molecular hybridization with cloned HAV cDNA. Hybridization was approximately 10-fold more sensitive than immune electron microscopy or radioimmunoassay was and less sensitive than was assays of HAV infectivity in primates or in cell culture. As little as 10(3) 50% infective doses of HAV, or approximately 0.1 pg of viral RNA, was detected by this method. Analysis of fecal specimens from an experimentally infected marmoset and an epidemic of hepatitis A showed that HAV excretion could often be detected later in the illness by hybridization than by radioimmunoassay. This technique should be widely applicable for detection and analysis of HAV RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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