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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Oct;25(10):1926–1931. doi: 10.1128/jcm.25.10.1926-1931.1987

Toxoplasma gondii antigens recognized by sequential samples of serum obtained from congenitally infected infants.

I Potasman 1, F G Araujo 1, P Thulliez 1, G Desmonts 1, J S Remington 1
PMCID: PMC269369  PMID: 3667913

Abstract

The Sabin-Feldman dye test, the immunoglobulin M (IgM) immunosorbent agglutination assay, and the immunoblot technique were used to study the evolution of the antibody response to Toxoplasma gondii and to examine antigens of the organism recognized by antibodies in the sera of 12 congenitally infected infants and 7 mothers. In the sera of eight infants, a significant rise was noted in the dye test titers, while the serum of only one infant demonstrated a late increase in the IgM immunosorbent agglutination assay titer. In each infant and mother, antigens with approximate masses of 35,000 and 115,000 daltons were strongly recognized by IgG antibodies. An antigen(s) with an approximate mass of 4,000 daltons was recognized by IgM antibodies in the sera of each of the mothers but was recognized by antibodies in the sera of only two of the infants.

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Selected References

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  1. COUVREUR J., DESMONTS G. Congenital and maternal toxoplasmosis. A review of 300 congenital cases. Dev Med Child Neurol. 1962 Oct;4:519–530. [PubMed] [Google Scholar]
  2. Desmonts G., Couvreur J. Congenital toxoplasmosis. A prospective study of 378 pregnancies. N Engl J Med. 1974 May 16;290(20):1110–1116. doi: 10.1056/NEJM197405162902003. [DOI] [PubMed] [Google Scholar]
  3. Desmonts G., Couvreur J. Toxoplasmose congénitale. Etude prospective de l'issue de la grossesse chez 542 femmes atteintes de toxoplasmose acquise en cours de gestation. Ann Pediatr (Paris) 1984 Nov;31(10):805–809. [PubMed] [Google Scholar]
  4. Desmonts G., Naot Y., Remington J. S. Immunoglobulin M-immunosorbent agglutination assay for diagnosis of infectious diseases: diagnosis of acute congenital and acquired Toxoplasma infections. J Clin Microbiol. 1981 Nov;14(5):486–491. doi: 10.1128/jcm.14.5.486-491.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnson A. M., McDonald P. J., Neoh S. H. Molecular weight analysis of soluble antigens from Toxoplasma gondii. J Parasitol. 1983 Jun;69(3):459–464. [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Naot Y., Remington J. S. An enzyme-linked immunosorbent assay for detection of IgM antibodies to Toxoplasma gondii: use for diagnosis of acute acquired toxoplasmosis. J Infect Dis. 1980 Nov;142(5):757–766. doi: 10.1093/infdis/142.5.757. [DOI] [PubMed] [Google Scholar]
  8. Partanen P., Turunen H. J., Paasivuo R. T., Leinikki P. O. Immunoblot analysis of Toxoplasma gondii antigens by human immunoglobulins G, M, and A antibodies at different stages of infection. J Clin Microbiol. 1984 Jul;20(1):133–135. doi: 10.1128/jcm.20.1.133-135.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Potasman I., Araujo F. G., Desmonts G., Remington J. S. Analysis of Toxoplasma gondii antigens recognized by human sera obtained before and after acute infection. J Infect Dis. 1986 Oct;154(4):650–657. doi: 10.1093/infdis/154.4.650. [DOI] [PubMed] [Google Scholar]
  10. Potasman I., Araujo F. G., Remington J. S. Toxoplasma antigens recognized by naturally occurring human antibodies. J Clin Microbiol. 1986 Dec;24(6):1050–1054. doi: 10.1128/jcm.24.6.1050-1054.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Remington J. S., Araujo F. G., Desmonts G. Recognition of different Toxoplasma antigens by IgM and IgG antibodies in mothers and their congenitally infected newborns. J Infect Dis. 1985 Nov;152(5):1020–1024. doi: 10.1093/infdis/152.5.1020. [DOI] [PubMed] [Google Scholar]
  12. Sabin A. B., Feldman H. A. Dyes as Microchemical Indicators of a New Immunity Phenomenon Affecting a Protozoon Parasite (Toxoplasma). Science. 1948 Dec 10;108(2815):660–663. doi: 10.1126/science.108.2815.660. [DOI] [PubMed] [Google Scholar]
  13. Sharma S. D., Mullenax J., Araujo F. G., Erlich H. A., Remington J. S. Western Blot analysis of the antigens of Toxoplasma gondii recognized by human IgM and IgG antibodies. J Immunol. 1983 Aug;131(2):977–983. [PubMed] [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

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