Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Oct;25(10):1985–1988. doi: 10.1128/jcm.25.10.1985-1988.1987

Improved selective culture media for Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus.

A Holm 1, P Rabe 1, S Kalfas 1, S Edwardsson 1
PMCID: PMC269382  PMID: 3667919

Abstract

By modifying the previously described media tryptic soy-serum-bacitracin-vancomycin (TSBV) agar and tryptic soy-serum-bacitracin-vancomycin-fluoride (TSBVF) agar, two improved selective culture media were developed for isolation and enumeration of Actinobacillus actinomycetemcomitans (A medium) and Haemophilus aphrophilus (H medium) in oral specimens. Both media were supplemented with fusidic acid and spiramycin, and carbenicillin was also added to A medium. The growth yields of pure cultures of A. actinomycetemcomitans on A medium and of H. aphrophilus on H medium were comparable with those on the reference media. Compared with blood agar, the selective media inhibited these species about 10-fold or less. In addition, A and H media suppressed the growth of pure cultures of Capnocytophaga spp. and Neisseria spp., commonly found as contaminants on TSBV and TSBVF, 10(5) times or more compared with that on blood agar. In samples from diseased periodontal pockets, the recoveries of A. actinomycetemcomitans on A medium and H. aphrophilus on H medium equaled those on TSBV and TSBVF, respectively. In about 50% of the cultures on the reference media, contaminating bacteria were detected at levels higher than 10(4) CFU/ml of sample. The corresponding value for both A and H media was about 2%.

Full text

PDF
1985

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker P. J., Evans R. T., Slots J., Genco R. J. Susceptibility of human oral anaerobic bacteria to antibiotics suitable for topical use. J Clin Periodontol. 1985 Mar;12(3):201–208. doi: 10.1111/j.1600-051x.1985.tb00917.x. [DOI] [PubMed] [Google Scholar]
  2. Carlsson J., Edlund M. B., Hänström L. Bactericidal and cytotoxic effects of hypothiocyanite-hydrogen peroxide mixtures. Infect Immun. 1984 Jun;44(3):581–586. doi: 10.1128/iai.44.3.581-586.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Christersson L. A., Slots J., Zambon J. J., Genco R. J. Transmission and colonization of Actinobacillus actinomycetemcomitans in localized juvenile periodontitis patients. J Periodontol. 1985 Mar;56(3):127–131. doi: 10.1902/jop.1985.56.3.127. [DOI] [PubMed] [Google Scholar]
  4. Hovig B., Aandahl E. H. A selective method for the isolation of Haemophilus in material from the respiratory tract. Acta Pathol Microbiol Scand. 1969;77(4):676–684. doi: 10.1111/j.1699-0463.1969.tb04510.x. [DOI] [PubMed] [Google Scholar]
  5. Kalfas S., Edwardsson S. Agar media that indicate acid production from sorbitol by oral microorganisms. J Clin Microbiol. 1985 Dec;22(6):959–961. doi: 10.1128/jcm.22.6.959-961.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kilian M., Schiott C. R. Haemophili and related bacteria in the human oral cavity. Arch Oral Biol. 1975 Dec;20(12):791–796. doi: 10.1016/0003-9969(75)90055-2. [DOI] [PubMed] [Google Scholar]
  7. Kraut M. S., Attebery H. R., Finegold S. M., Sutter V. L. Detection of Haemophilus aphrophilus in the human oral flora with a selective medium. J Infect Dis. 1972 Aug;126(2):189–192. doi: 10.1093/infdis/126.2.189. [DOI] [PubMed] [Google Scholar]
  8. Laughon B. E., Syed S. A., Loesche W. J. API ZYM system for identification of Bacteroides spp., Capnocytophaga spp., and spirochetes of oral origin. J Clin Microbiol. 1982 Jan;15(1):97–102. doi: 10.1128/jcm.15.1.97-102.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leadbetter E. R., Holt S. C., Socransky S. S. Capnocytophaga: new genus of gram-negative gliding bacteria. I. General characteristics, taxonomic considerations and significance. Arch Microbiol. 1979 Jul;122(1):9–16. doi: 10.1007/BF00408040. [DOI] [PubMed] [Google Scholar]
  10. Liljemark W. F., Bloomquist C. G., Uhl L. A., Schaffer E. M., Wolff L. F., Pihlstrom B. L., Bandt C. L. Distribution of oral Haemophilus species in dental plaque from a large adult population. Infect Immun. 1984 Dec;46(3):778–786. doi: 10.1128/iai.46.3.778-786.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moore W. E., Holdeman L. V., Smibert R. M., Hash D. E., Burmeister J. A., Ranney R. R. Bacteriology of severe periodontitis in young adult humans. Infect Immun. 1982 Dec;38(3):1137–1148. doi: 10.1128/iai.38.3.1137-1148.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Newman M. G., Socransky S. S. Predominant cultivable microbiota in periodontosis. J Periodontal Res. 1977 Mar;12(2):120–128. doi: 10.1111/j.1600-0765.1977.tb00114.x. [DOI] [PubMed] [Google Scholar]
  13. Slots J., Reynolds H. S., Genco R. J. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun. 1980 Sep;29(3):1013–1020. doi: 10.1128/iai.29.3.1013-1020.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Slots J. Salient Biochemical Characters of Actinobacillus actinomycetemcomitans. Arch Microbiol. 1982 Feb;131(1):60–67. doi: 10.1007/BF00451500. [DOI] [PubMed] [Google Scholar]
  15. Slots J. Selective medium for isolation of Actinobacillus actinomycetemcomitans. J Clin Microbiol. 1982 Apr;15(4):606–609. doi: 10.1128/jcm.15.4.606-609.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Slots J. Subgingival microflora and periodontal disease. J Clin Periodontol. 1979 Oct;6(5):351–382. doi: 10.1111/j.1600-051x.1979.tb01935.x. [DOI] [PubMed] [Google Scholar]
  17. Sutter V. L., Finegold S. M. Haemophilus aphrophilus infections: clinical and bacteriologic studies. Ann N Y Acad Sci. 1970 Oct 30;174(2):468–487. doi: 10.1111/j.1749-6632.1970.tb45574.x. [DOI] [PubMed] [Google Scholar]
  18. Tempro P. J., Slots J. Selective medium for the isolation of Haemophilus aphrophilus from the human periodontium and other oral sites and the low proportion of the organism in the oral flora. J Clin Microbiol. 1986 Apr;23(4):777–782. doi: 10.1128/jcm.23.4.777-782.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zambon J. J. Actinobacillus actinomycetemcomitans in human periodontal disease. J Clin Periodontol. 1985 Jan;12(1):1–20. doi: 10.1111/j.1600-051x.1985.tb01348.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES