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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Traffic. 2008 Nov 18;10(2):201–217. doi: 10.1111/j.1600-0854.2008.00856.x

Figure 9. STAM2 recruitment to the early secretory pathway is dependent on Sar1 activity, but not on Sec31.

Figure 9

A) Lysates from HeLa cells transfected with any of three different Sec31A siRNAs or else control siRNA were immunoblotted for Sec31A 72 h post-transfection. Equal protein loading was monitored using PLCγ. B) HeLa cells were co-stained with antibodies against STAM2 and Sec31A after transfection with control or Sec31A siRNAs; there is no change in STAM2 distribution upon Sec31A depletion. C) Control HeLa cells were stained for STAM2 or Sec31A (left panels). Cells transfected with HA-Sar1 [H79G] (HA-epitope staining in insets) show changes in distribution patterns for both proteins, with STAM2 localizing to large punctate structures and Sec31A labeling becoming more condensed (middle panels). Cells expressing HA-Sar1 [T39N] (HA-epitope staining in insets) show decreased staining intensity of puncta and dispersal of immunoreactivity for both STAM2 and Sec31A (right panels).