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. 1987 Nov;25(11):2049–2053. doi: 10.1128/jcm.25.11.2049-2053.1987

Evaluation of MUREX SUDS Toxo test.

N P Moyer 1, J D Hudson 1, W J Hausler Jr 1
PMCID: PMC269409  PMID: 3320079

Abstract

The SUDS Toxo test (MUREX Corp., Norcross, Ga.) was compared with the indirect hemagglutination test (IHA) and the indirect fluorescent-antibody test (IFA) by examining 404 serum specimens, including 64 (15.8%) specimens with IFA titers of greater than or equal to 1:2. When SUDS was compared with IHA, sensitivity (96.4%), specificity (97.9%), and negative predictive value (99.4%) indicated that there were similar reactivities between the two tests. When an IFA titer of greater than or equal to 1:16 was considered significant and IHA and SUDS were compared with IFA, IHA was slightly less sensitive but had a higher positive predictive value than did SUDS; however, there was no statistical difference between the tests. When SUDS was compared with IFA, in which a titer of greater than or equal to 1:16 was considered significant, the high negative predictive value (100%), excellent sensitivity (100%) and specificity (98.3%), and ease of performance made SUDS an attractive alternative to IHA for screening single serum specimens for toxoplasmosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balsari A., Poli G., Molina V., Dovis M., Petruzzelli E., Boniolo A., Rolleri E. ELISA for toxoplasma antibody detection: a comparison with other serodiagnostic tests. J Clin Pathol. 1980 Jul;33(7):640–643. doi: 10.1136/jcp.33.7.640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHORDI A., WALLS K. W., KAGAN I. G. STUDIES ON THE SPECIFICITY OF THE INDIRECT HEMAGGLUTINATION TEST FOR TOXOPLASMOSIS. J Immunol. 1964 Dec;93:1024–1033. [PubMed] [Google Scholar]
  3. Desmonts G., Couvreur J. Congenital toxoplasmosis. A prospective study of 378 pregnancies. N Engl J Med. 1974 May 16;290(20):1110–1116. doi: 10.1056/NEJM197405162902003. [DOI] [PubMed] [Google Scholar]
  4. FELDMAN H. A., MILLER L. T. Serological study of toxoplasmosis prevalence. Am J Hyg. 1956 Nov;64(3):320–335. doi: 10.1093/oxfordjournals.aje.a119845. [DOI] [PubMed] [Google Scholar]
  5. Hyde B., Barnett E. V., Remington J. S. Method for differentiation of nonspecific from specfic toxoplasma IgM fluorescent antibodies in patients with rheumatoid factor. Proc Soc Exp Biol Med. 1975 Apr;148(4):1184–1188. doi: 10.3181/00379727-148-38713. [DOI] [PubMed] [Google Scholar]
  6. JACOBS L., LUNDE M. N. A hemagglutination test for toxoplasmosis. J Parasitol. 1957 Jun;43(3):308–314. [PubMed] [Google Scholar]
  7. Konishi E., Takahashi J. Reproducible enzyme-linked immunosorbent assay with a magnetic processing system for diagnosis of toxoplasmosis. J Clin Microbiol. 1983 Feb;17(2):225–231. doi: 10.1128/jcm.17.2.225-231.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lin T. M., Halbert S. P., O'Connor G. R. Standardized quantitative enzyme-linked immunoassay for antibodies to Toxoplasma gondii. J Clin Microbiol. 1980 Jun;11(6):675–681. doi: 10.1128/jcm.11.6.675-681.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Naot Y., Barnett E. V., Remington J. S. Method for avoiding false-positive results occurring in immunoglobulin M enzyme-linked immunosorbent assays due to presence of both rheumatoid factor and antinuclear antibodies. J Clin Microbiol. 1981 Jul;14(1):73–78. doi: 10.1128/jcm.14.1.73-78.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Naot Y., Remington J. S. An enzyme-linked immunosorbent assay for detection of IgM antibodies to Toxoplasma gondii: use for diagnosis of acute acquired toxoplasmosis. J Infect Dis. 1980 Nov;142(5):757–766. doi: 10.1093/infdis/142.5.757. [DOI] [PubMed] [Google Scholar]
  11. Sabin A. B., Feldman H. A. Dyes as Microchemical Indicators of a New Immunity Phenomenon Affecting a Protozoon Parasite (Toxoplasma). Science. 1948 Dec 10;108(2815):660–663. doi: 10.1126/science.108.2815.660. [DOI] [PubMed] [Google Scholar]
  12. Turunen H. J., Leinikki P. O., Saari K. M. Demonstration of intraocular synthesis of immunoglobulin G toxoplasma antibodies for specific diagnosis of toxoplasmic chorioretinitis by enzyme immunoassay. J Clin Microbiol. 1983 Jun;17(6):988–992. doi: 10.1128/jcm.17.6.988-992.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Violand S. A., Mitchell T. G., Kleeman K. T. Comparison of an enzyme-linked immunoassay and a quantitative indirect fluorescent-antibody test with the conventional indirect fluorescent-antibody test for detecting antibodies to Toxoplasma gondii. J Clin Microbiol. 1982 Aug;16(2):341–344. doi: 10.1128/jcm.16.2.341-344.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Voller A., Bidwell D. E., Bartlett A., Fleck D. G., Perkins M., Oladehin B. A microplate enzyme-immunoassay for toxoplasma antibody. J Clin Pathol. 1976 Feb;29(2):150–153. doi: 10.1136/jcp.29.2.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Walls K. W., Bullock S. L., English D. K. Use of the enzyme-linked immunosorbent assay (ELISA) and its microadaptation for the serodiagnosis of toxoplasmosis. J Clin Microbiol. 1977 Mar;5(3):273–277. doi: 10.1128/jcm.5.3.273-277.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Walton B. C., Benchoff B. M., Brooks W. H. Comparison of the indirect fluorescent antibody test and methylene blue dye test for detection of antibodies to Toxoplasma gondii. Am J Trop Med Hyg. 1966 Mar;15(2):149–152. doi: 10.4269/ajtmh.1966.15.149. [DOI] [PubMed] [Google Scholar]
  17. Wielaard F., van Gruijthuijsen H., Duermeyer W., Joss A. W., Skinner L., Williams H., van Elven E. H. Diagnosis of acute toxoplasmosis by an enzyme immunoassay for specific immunoglobulin m antibodies. J Clin Microbiol. 1983 Jun;17(6):981–987. doi: 10.1128/jcm.17.6.981-987.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Woodward B. C. Evaluation of an enzyme-linked immunosorbent assay specific for human immunoglobulin G as a Screening test for detecting anti-toxoplasma antibodies. J Clin Microbiol. 1982 Aug;16(2):367–372. doi: 10.1128/jcm.16.2.367-372.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van Loon A. M., van der Logt J. T., Heessen F. W., van der Veen J. Enzyme-linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin M and A antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double-sandwich enzyme-linked immunosorbent assay. J Clin Microbiol. 1983 Jun;17(6):997–1004. doi: 10.1128/jcm.17.6.997-1004.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. van Loon A. M., van der Veen J. Enzyme-linked immunosorbent assay for quantitation of toxoplasma antibodies in human sera. J Clin Pathol. 1980 Jul;33(7):635–639. doi: 10.1136/jcp.33.7.635. [DOI] [PMC free article] [PubMed] [Google Scholar]

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