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. 1987 Nov;25(11):2150–2153. doi: 10.1128/jcm.25.11.2150-2153.1987

Detection of Salmonella spp. in clinical specimens by capture enzyme-linked immunosorbent assay.

G F Araj 1, T D Chugh 1
PMCID: PMC269430  PMID: 3693545

Abstract

A capture enzyme-linked immunosorbent assay (ELISA; Kirkegaard and Perry Laboratories, Gaithersburg, Md.) was used to detect Salmonella spp. in clinical and artificially inoculated specimens. In patients with bacteremia caused by Salmonella spp., 48% (12 of 25) and 82% (13 of 16) of serum and urine specimens, respectively, were positive for Salmonella spp., as determined by ELISA. All serum and urine specimens collected from healthy individuals (25 specimens) or patients whose blood cultures grew gram-negative bacteria other than Salmonella spp. (18 specimens) were negative for Salmonella spp., as determined by ELISA. For blood culture bottles in which Salmonella spp. (16 specimens) was grown the ELISA was positive (100%), while it was negative for all the 65 blood culture bottles in which gram-negative bacteria other than Salmonella spp. (42 specimens) or gram-positive bacteria (23 specimens) were grown. All samples of urine (16 specimens), stool (8 specimens), serum (16 specimens), culture media (12 specimens), and blood culture bottles (reported sterile after 2 weeks of incubation; 16 specimens) that were artificially inoculated with 10(3) to 10(7) CFU of four species of Salmonella per ml were positive by ELISA. Similar specimens inoculated with or containing various species other than Salmonella were negative by this test. Thus, ELISA offers a promising opportunity for the rapid detection of Salmonella spp. in clinical microbiology laboratories.

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Selected References

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  1. Abraham G., Teklu B., Gedebu M., Selassie G. H., Azene G. Diagnostic value of the Widal test. Trop Geogr Med. 1981 Dec;33(4):329–333. [PubMed] [Google Scholar]
  2. Barrett T. J., Blake P. A., Brown S. L., Hoffman K., Llort J. M., Feeley J. C. Enzyme-linked immunosorbent assay for detection of human antibodies to Salmonella typhi Vi antigen. J Clin Microbiol. 1983 Apr;17(4):625–627. doi: 10.1128/jcm.17.4.625-627.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barrett T. J., Snyder J. D., Blake P. A., Feeley J. C. Enzyme-linked immunosorbent assay for detection of Salmonella typhi Vi antigen in urine from typhoid patients. J Clin Microbiol. 1982 Feb;15(2):235–237. doi: 10.1128/jcm.15.2.235-237.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beasley W. J., Joseph S. W., Weiss E. Improved serodiagnosis of Salmonella enteric fevers by an enzyme-linked immunosorbent assay. J Clin Microbiol. 1981 Jan;13(1):106–114. doi: 10.1128/jcm.13.1.106-114.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doshi N., Taylor A. G. Comparison of the Vi indirect fluorescent antibody test with the Widal agglutination method in the serodiagnosis of typhoid fever. J Clin Pathol. 1984 Jul;37(7):805–808. doi: 10.1136/jcp.37.7.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edelman R., Levine M. M. Summary of an international workshop on typhoid fever. Rev Infect Dis. 1986 May-Jun;8(3):329–349. doi: 10.1093/clinids/8.3.329. [DOI] [PubMed] [Google Scholar]
  7. Krysinski E. P., Heimsch R. C. Use of enzyme-labeled antibodies to detect Salmonella in foods. Appl Environ Microbiol. 1977 Apr;33(4):947–954. doi: 10.1128/aem.33.4.947-954.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Levine M. M., Grados O., Gilman R. H., Woodward W. E., Solis-Plaza R., Waldman W. Diagnostic value of the Widal test in areas endemic for typhoid fever. Am J Trop Med Hyg. 1978 Jul;27(4):795–800. doi: 10.4269/ajtmh.1978.27.795. [DOI] [PubMed] [Google Scholar]
  9. Nardiello S., Pizzella T., Russo M., Galanti B. Serodiagnosis of typhoid fever by enzyme-linked immunosorbent assay determination of anti-Salmonella typhi lipopolysaccharide antibodies. J Clin Microbiol. 1984 Oct;20(4):718–721. doi: 10.1128/jcm.20.4.718-721.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rigby C. E. Enzyme-linked immunosorbent assay for detection of Salmonella lipopolysaccharide in poultry specimens. Appl Environ Microbiol. 1984 Jun;47(6):1327–1330. doi: 10.1128/aem.47.6.1327-1330.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Robison B. J., Pretzman C. I., Mattingly J. A. Enzyme immunoassay in which a myeloma protein is used for detection of salmonellae. Appl Environ Microbiol. 1983 Jun;45(6):1816–1821. doi: 10.1128/aem.45.6.1816-1821.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rockhill R. C., Rumans L. W., Lesmana M., Dennis D. T. Detection of Salmonella typhi D, Vi, and d antigens, by slide coagglutination, in urine from patients with typhoid fever. J Clin Microbiol. 1980 Mar;11(3):213–216. doi: 10.1128/jcm.11.3.213-216.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sivadasan K., Kurien B., John T. J. Rapid diagnosis of typhoid fever by antigen detection. Lancet. 1984 Jan 21;1(8369):134–135. doi: 10.1016/s0140-6736(84)90064-3. [DOI] [PubMed] [Google Scholar]
  14. Sundararaj T., Ilango B., Subramanian S. A study on the usefulness of counter immuno-electrophoresis for the detection of Salmonella typhi antigen in the sera of suspected cases of enteric fever. Trans R Soc Trop Med Hyg. 1983;77(2):194–197. doi: 10.1016/0035-9203(83)90067-6. [DOI] [PubMed] [Google Scholar]
  15. Taylor D. N., Harris J. R., Barrett T. J., Hargrett N. T., Prentzel I., Valdivieso C., Palomino C., Levine M. M., Blake P. A. Detection of urinary Vi antigen as a diagnostic test for typhoid fever. J Clin Microbiol. 1983 Oct;18(4):872–876. doi: 10.1128/jcm.18.4.872-876.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tsang R. S., Chau P. Y., Lam S. K., La Brooy J. T., Rowley D. Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. I. Measurement of serum antibodies by radioimmunoassay. Clin Exp Immunol. 1981 Dec;46(3):508–514. [PMC free article] [PubMed] [Google Scholar]
  17. Wicks A. C., Holmes G. S., Davidson L. Endemic typhoid fever. A diagnostic pitfall. Q J Med. 1971 Jul;40(159):341–354. [PubMed] [Google Scholar]
  18. Yolken R. H. Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects. Rev Infect Dis. 1982 Jan-Feb;4(1):35–68. doi: 10.1093/clinids/4.1.35. [DOI] [PubMed] [Google Scholar]
  19. Zuerlein T. J., Smith P. W. The diagnostic utility of the febrile agglutinin tests. JAMA. 1985 Sep 6;254(9):1211–1214. [PubMed] [Google Scholar]

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