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. 1987 Nov;25(11):2227–2229. doi: 10.1128/jcm.25.11.2227-2229.1987

Rapid lysis technique for mycobacterial species.

S S Hurley 1, G A Splitter 1, R A Welch 1
PMCID: PMC269451  PMID: 2447121

Abstract

The lysis of mycobacterial cells typically has been difficult and time-consuming. We report a method for the physical rupture of Mycobacterium paratuberculosis and several other members of the family Mycobacteriaceae using a Mini-Beadbeater cell disrupter (Biospec Products; Bartlesville, Okla.) and zirconium beads, a process which yields DNA and RNA of high molecular weight and in greater quantity than that obtained by rupture in a Ribi pressure cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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