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. 1987 Dec;25(12):2281–2284. doi: 10.1128/jcm.25.12.2281-2284.1987

Subtyping of Legionella pneumophila serogroup 1 isolates by monoclonal antibody and plasmid techniques.

W E Maher 1, M F Para 1, J F Plouffe 1
PMCID: PMC269471  PMID: 2828413

Abstract

A group of environmental and clinical Legionella pneumophila serogroup 1 isolates was subtyped by monoclonal antibody dot immunoblotting and plasmid analysis. Monoclonal antibody analysis defined seven subtypes within three major groups. Plasmid analysis (including restriction endonuclease digestion) revealed 10 subtypes. By combining plasmid and monoclonal techniques, all 16 strains were shown to be distinct. Plasmid profiles and monoclonal antibody reactivities of selected strains were stable despite serial passage (greater than 100 times). No plasmid-associated antigen was defined by this panel of monoclonal antibodies. The observed dissociation of plasmid profiles and monoclonal antibody reactivity patterns suggests that accurate epidemiologic typing of L. pneumophila serogroup 1 strains will require use of both techniques.

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Selected References

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  1. Arnow P. M., Weil D., Para M. F. Prevalence and significance of Legionella pneumophila contamination of residential hot-tap water systems. J Infect Dis. 1985 Jul;152(1):145–151. doi: 10.1093/infdis/152.1.145. [DOI] [PubMed] [Google Scholar]
  2. Brown A., Lema M., Ciesielski C. A., Blaser M. J. Combined plasmid and peptide analysis of clinical and environmental Legionella pneumophila strains associated with a small cluster of Legionnaires' disease cases. Infection. 1985 Jul-Aug;13(4):163–166. doi: 10.1007/BF01642803. [DOI] [PubMed] [Google Scholar]
  3. Brown A., Vickers R. M., Elder E. M., Lema M., Garrity G. M. Plasmid and surface antigen markers of endemic and epidemic Legionella pneumophila strains. J Clin Microbiol. 1982 Aug;16(2):230–235. doi: 10.1128/jcm.16.2.230-235.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown S. L., Bibb W. F., McKinney R. M. Use of monoclonal antibodies in an epidemiological marker system: a retrospective study of lung specimens from the 1976 outbreak of Legionnaires disease in Philadelphia by indirect fluorescent-antibody and enzyme-linked immunosorbent assay methods. J Clin Microbiol. 1985 Jan;21(1):15–19. doi: 10.1128/jcm.21.1.15-19.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buesching W. J., Brust R. A., Ayers L. W. Enhanced primary isolation of Legionella pneumophila from clinical specimens by low-pH treatment. J Clin Microbiol. 1983 Jun;17(6):1153–1155. doi: 10.1128/jcm.17.6.1153-1155.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edelstein P. H., Nakahama C., Tobin J. O., Calarco K., Beer K. B., Joly J. R., Selander R. K. Paleoepidemiologic investigation of Legionnaires disease at Wadsworth Veterans Administration Hospital by using three typing methods for comparison of legionellae from clinical and environmental sources. J Clin Microbiol. 1986 Jun;23(6):1121–1126. doi: 10.1128/jcm.23.6.1121-1126.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garbe P. L., Davis B. J., Weisfeld J. S., Markowitz L., Miner P., Garrity F., Barbaree J. M., Reingold A. L. Nosocomial Legionnaires' disease. Epidemiologic demonstration of cooling towers as a source. JAMA. 1985 Jul 26;254(4):521–524. doi: 10.1001/jama.254.4.521. [DOI] [PubMed] [Google Scholar]
  8. Hawkes R., Niday E., Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem. 1982 Jan 1;119(1):142–147. doi: 10.1016/0003-2697(82)90677-7. [DOI] [PubMed] [Google Scholar]
  9. Johnston J. M., Latham R. H., Meier F. A., Green J. A., Boshard R., Mooney B. R., Edelstein P. H. Nosocomial outbreak of Legionnaires' disease: molecular epidemiology and disease control measures. Infect Control. 1987 Feb;8(2):53–58. doi: 10.1017/s0195941700067072. [DOI] [PubMed] [Google Scholar]
  10. Joly J. R., McKinney R. M., Tobin J. O., Bibb W. F., Watkins I. D., Ramsay D. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol. 1986 Apr;23(4):768–771. doi: 10.1128/jcm.23.4.768-771.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Joly J. R., Winn W. C. Correlation of subtypes of Legionella pneumophila defined by monoclonal antibodies with epidemiological classification of cases and environmental sources. J Infect Dis. 1984 Nov;150(5):667–671. doi: 10.1093/infdis/150.5.667. [DOI] [PubMed] [Google Scholar]
  12. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maher W. E., Plouffe J. F., Para M. F. Plasmid profiles of clinical and environmental isolates of Legionella pneumophila serogroup 1. J Clin Microbiol. 1983 Dec;18(6):1422–1423. doi: 10.1128/jcm.18.6.1422-1423.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McKinney R. M., Thacker L., Wells D. E., Wong M. C., Jones W. J., Bibb W. F. Monoclonal antibodies to Legionella pneumophila serogroup 1: possible applications in diagnostic tests and epidemiologic studies. Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Jul;255(1):91–95. [PubMed] [Google Scholar]
  15. Meenhorst P. L., Reingold A. L., Groothuis D. G., Gorman G. W., Wilkinson H. W., McKinney R. M., Feeley J. C., Brenner D. J., van Furth R. Water-related nosocomial pneumonia caused by Legionella pneumophila serogroups 1 and 10. J Infect Dis. 1985 Aug;152(2):356–364. doi: 10.1093/infdis/152.2.356. [DOI] [PubMed] [Google Scholar]
  16. Neill M. A., Gorman G. W., Gibert C., Roussel A., Hightower A. W., McKinney R. M., Broome C. V. Nosocomial legionellosis, Paris, France. Evidence for transmission by potable water. Am J Med. 1985 Apr;78(4):581–588. doi: 10.1016/0002-9343(85)90399-7. [DOI] [PubMed] [Google Scholar]
  17. Nolte F. S., Conlin C. A., Roisin A. J., Redmond S. R. Plasmids as epidemiological markers in nosocomial Legionnaires' disease. J Infect Dis. 1984 Feb;149(2):251–256. doi: 10.1093/infdis/149.2.251. [DOI] [PubMed] [Google Scholar]
  18. Para M. F., Plouffe J. F. Production of monoclonal antibodies to Legionella pneumophila serogroups 1 and 6. J Clin Microbiol. 1983 Oct;18(4):895–900. doi: 10.1128/jcm.18.4.895-900.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Plouffe J. F., Para M. F., Maher W. E., Hackman B., Webster L. Subtypes of Legionella pneumophila serogroup 1 associated with different attack rates. Lancet. 1983 Sep 17;2(8351):649–650. doi: 10.1016/s0140-6736(83)92531-x. [DOI] [PubMed] [Google Scholar]
  20. Selander R. K., McKinney R. M., Whittam T. S., Bibb W. F., Brenner D. J., Nolte F. S., Pattison P. E. Genetic structure of populations of Legionella pneumophila. J Bacteriol. 1985 Sep;163(3):1021–1037. doi: 10.1128/jb.163.3.1021-1037.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shlaes D. M., Currie C. A. Endemic gentamicin resistance R factors on a spinal cord injury unit. J Clin Microbiol. 1983 Aug;18(2):236–241. doi: 10.1128/jcm.18.2.236-241.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stout J. E., Yu V. L., Muraca P. Legionnaires' disease acquired within the homes of two patients. Link to the home water supply. JAMA. 1987 Mar 6;257(9):1215–1217. [PubMed] [Google Scholar]
  23. Tompkins L. S., Plorde J. J., Falkow S. Molecular analysis of R-factors from multiresistant nosocomial isolates. J Infect Dis. 1980 May;141(5):625–636. doi: 10.1093/infdis/141.5.625. [DOI] [PubMed] [Google Scholar]
  24. Watkins I. D., Tobin J. O., Dennis P. J., Brown W., Newnham R., Kurtz J. B. Legionella pneumophila serogroup 1 subgrouping by monoclonal antibodies--an epidemiological tool. J Hyg (Lond) 1985 Oct;95(2):211–216. doi: 10.1017/s0022172400062641. [DOI] [PMC free article] [PubMed] [Google Scholar]

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